Polyclonal are those when serum contains different ab against single antigen for different epitope and each one is specific for one epitope. I think you might have told unconsciously that one ab recognise different epitope.
You have the general idea right but off when you get into specifics. For a start, there is no such thing as a polyclonal antibody. It would be synonymous to saying someone has multiple birth mothers! Impossible right?! Polyclonal is the mixture of antibodies from multiple cells before clone selection. Yes, the mixture will target different epitopes but on a micro level, each antibody can only be specific to a single epitope.
Polyclonal antibody is actually collection of antibodies produced from different B cell lineages that's why they can interact with different epitopes of same antigen. Am I right?
I read this remark like 2,3 times here (@MegaMie77, @spandita roy), so for clarification, as I understand it: If you inject a specimen A (let's say a mouse) with a specific antigen, this host will maturate lymphatic B-cells into B-plasma cells (through the known pathway macrophages - dentritic cells - lymphatic T-cells - T-helper cells) that produce antibodies against it. These Ab can be extracted as a serum. Normally, an antigen has more than one epitope with which an antibody can interact. Therefore, the extracted serum/mixture would contain different antibodies against the antigen, (as a whole) so-called polyclonal antibodies. Still, each of the antibodies can only interact with one specific epitope expressed on said antigen. Hope that helps/is at least accurate :D
U explain really great I have 10 days for my enterence exam and with your videos i have completed so many things in less time and u explain in such a simple way tht it's really easy to remember
you are really simplifier, and recently detected your Chanel accidentally, and it's now one of my first sources for referring when things go tough in biology . Thanks
Indeed the man is very great, I've been watching his videos since my 1st year, I'm now in 3rd year doing Veterinary Medicine at the University of Zambia 🇿🇲.
You are saying, a polyclonal antibody is the one which can bind with different types of epitopes? 3:55 . I didnt get this part. (Maybe i learned wrong then!)
Different epitopes means different regions of the antibody, those make bond with antigen. For better understanding you must know the molecular structure of the antigens.
actually polyclonal antibodies are mixture of monoclonal antibodies....which bind to specific epitope but since they are mixture...so they bind to various specific epitopes.....
Polyclonal are those when serum contains different ab against single antigen for different epitope and each one is specific for one epitope. I think you might have told unconsciously that one ab recognise different epitope.
You have the general idea right but off when you get into specifics. For a start, there is no such thing as a polyclonal antibody. It would be synonymous to saying someone has multiple birth mothers! Impossible right?! Polyclonal is the mixture of antibodies from multiple cells before clone selection. Yes, the mixture will target different epitopes but on a micro level, each antibody can only be specific to a single epitope.
No, you can have cross reactivity.
Polyclonal antibody is actually collection of antibodies produced from different B cell lineages that's why they can interact with different epitopes of same antigen. Am I right?
Yes
Yes
I read this remark like 2,3 times here (@MegaMie77, @spandita roy), so for clarification, as I understand it:
If you inject a specimen A (let's say a mouse) with a specific antigen, this host will maturate lymphatic B-cells into B-plasma cells (through the known pathway macrophages - dentritic cells - lymphatic T-cells - T-helper cells) that produce antibodies against it. These Ab can be extracted as a serum. Normally, an antigen has more than one epitope with which an antibody can interact. Therefore, the extracted serum/mixture would contain different antibodies against the antigen, (as a whole) so-called polyclonal antibodies. Still, each of the antibodies can only interact with one specific epitope expressed on said antigen.
Hope that helps/is at least accurate :D
Sir u are really the great . If I will meet u then I will do the robbbery of ur mind😎
Ha ha
U explain really great
I have 10 days for my enterence exam and with your videos i have completed so many things in less time and u explain in such a simple way tht it's really easy to remember
Thank you so much for appreciating my efforts
Your insight is now becomes applicable to understand Antigen against covid 19. Thanks for your anticipation years back
Thank you. Glad you liked my lectures
Shabash
you are really simplifier, and recently detected your Chanel accidentally, and it's now one of my first sources for referring when things go tough in biology . Thanks
Thank you so much for appreciating
Indeed the man is very great, I've been watching his videos since my 1st year, I'm now in 3rd year doing Veterinary Medicine at the University of Zambia 🇿🇲.
God bless you , you explain in very simple and clear
Thank you. Glad you liked my lectures
Thank you sir for such a informational vedio .......❤️
You're welcome. Glad to hear that you're getting benefit from my lectures
Please make a video on antibody humanization
Is only one B cell producing different types of antibodies?
Love the way... You explained sir... Hats off Sir jee
Thank you so much for appreciating my efforts
Thank you Sir, this video was helpful
Sir
Again a short lecture about polyclonal and monoclonal Ab in hindi
Coming soon
Thank you!!!!!!!❤
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Ur great sir g
Thank you
u helped me teach better understanding of immunity and vaccines to my students.. thank u
You're welcome. Glad you like it
Nascarwalu phuk no bos tu
Why we can't use a single antibody as monoclonal antibody from the polyclonal antibody mixture?
Thank u so much sir... I got complete idea about it....
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thank you for the explanation
Specificity and affinity these are the unique words used in this video ❤️
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Sir ..is there any book in which this topic is present
Kuby immunology
@@shomusbiologyofficial thank u ...and for SDS page which book ...?
Sir could you please make one vidio about photo bioreactor
Sir is plasma cell and mast cells are one and the same
Sir u made many scientists lecturers 🙏
Thank you
Hindi me samjhaye sir g jayada samjh aayega or like v milega
Like nehi chahiye
So helpful❤️
Thank you
Very informative 😊
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Nice lecture sir. Easy to understand.
nice...explaination.and thank u...
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Thank you
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Excellent work
Thank you
thank you very much
You are saying, a polyclonal antibody is the one which can bind with different types of epitopes? 3:55 . I didnt get this part. (Maybe i learned wrong then!)
Different epitopes means different regions of the antibody, those make bond with antigen. For better understanding you must know the molecular structure of the antigens.
Good
Thank you
Nice
You're welcome
Nice
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❤❤👏
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Thanks bhai
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We wanna meet you.
Where are you from?
Fan from Karnataka 😍
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totally wrong concept
Why?
The audio of this vdo is not good...but the lecture is good 👍
actually polyclonal antibodies are mixture of monoclonal antibodies....which bind to specific epitope but since they are mixture...so they bind to various specific epitopes.....
thank you Sir......Now i'm cleared about mono and polyclonal antybody........thanks
Excellent presentation and explanation of mono/polyclonal antibodies..👍👍👍
thank you sir. i have a doubt. why antibody produced are type of IgG only?
Sir if we use tumor cell for multiplication is there any chance tumor formation
thank you for your easy explanation. big help here
thank you、 sir。how to choose the primary and secondary antibody？
Excellent way of teaching. Even a person from non biology background also can understand this well.
Thanks for your clarification,
your lectures are very useful sir thank u so much
thank you so much.. sir..can u post a video on nano technology and tissue engineering in medicine..
+pankaj k I will try to post videos on that topic. Thank you
sir can you post a video on vaccines and classification
+beena. r it's already there. You can find it 8n my channel