The actual species depends on where you are in the world, but in general I would say use a firmer leaf, and one that has no trichomes (hair or fuzz) on the underside. For trees, at least, the upper surface will have no stomata, or at best far fewer than the lower surface.
The peel is in fact from that leaf, but the image was not captured on that microscope (note the lack of a phototube). I filmed the peels using my research scope, which comes with Nomarski optics, which usually introduce interesting colors to unstained transparent materials. When you look at the peel without Nomarski optics, there is much less contrast and, at least on the Zeiss scopes students use (e.g., the one in the video), the peels only have as much color as the light source.
Probably, but the quality of the peel can suffer if the leaf surface is too waxy or trichome-dense, in which case you can try to remove the wax or hairs.
There are techniques that use safranin to stain the cuticle, but since this is an imprint of the cuticle, I'm not sure what benefit the safranin would provide.
Possibly; some glues definitely work better than others, so your best bet is to try one and see whether it works for you. All the stomatal images in this video were made of peels viewed under a compound microscope.
Here it's called clear nail polish; in the U.K. they may call it nail varnish. I've tried the clear water-based varnish mentioned in the video (brand Minwax), and actually prefer it to the nail polish.
Probably not without further attention, as the peels are taped directly onto the microscope slide. I suspect they can be made quite long-lasting by sealing the edges, e.g., with nail polish.
Water-based varnish works just as well, if not better. Don't know if you can bring that to a practical exam, either, but I didn't make the video with practical exams in mind.
Best Stomata video I've found. Thank you very much!!! It's crazy how little info there is about how plants (WHICH ARE EVERYWHERE) breathe.
Who else is here because of online school??
same online school is kinda good but kinda bad
I agree it’s so boring
Yeah online school is bad and boring.
me :/
lol me
This is great - thanks for making the video. Extremely helpful
Best video we found for our plant science study today. Thank you.
Thanks for making this video. I extremely need this
Wonderful, clear presentation!!
Thank you very much for this video ... very important and helpful practical informations
wow its awesome u have a pretty cool instruments
Thanks Dave !!!
Nice work man
Really great💉
Aapna acha sa samja ya ha sir very very very good
This is very helpful during quarantine period
Thanks a lot
great vid mannnnn
good teaching thanks
Great! Thank you 😊
Bggyuf f2f f v ggv
Gr8 wow....👍👍
Great 🌹🌹🌹🌹🌹🌹🌹🌹🌹👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌👌
I only searched for tomata.and came stomata😂😂
wow!!!!
how can we count the epidermal cell on it?
The trypophobia is real.
Easy method
Great shareing 😅😅😅😅
Awesome
Nice
Wich type of liquid you can use sir to for removal stomatal part of the leaf
You are making an imprint of the cuticle, you are not removing the stomatal layer (i.e., the cuticle) from the leaf.
❤❤
Thanks for the cool images and info. Medicinally grown cannabis is important. I made a few science based vids if you are interested. Smile on
I need that ruler
Which leaf is best for seeing stomata
The actual species depends on where you are in the world, but in general I would say use a firmer leaf, and one that has no trichomes (hair or fuzz) on the underside. For trees, at least, the upper surface will have no stomata, or at best far fewer than the lower surface.
Tradenscantia leaf
when he had his 2 fingers together I got serious avatar flashbacks
(1:00)
I did this at Uni today but didn't have tape so the peel ripped a bit :( still lots of fun
I agree, this is strangely gratifying!
Not bad, but I don't trust that the slide shown was the one he took off the leaf....'cause where's the green coming from?
The peel is in fact from that leaf, but the image was not captured on that microscope (note the lack of a phototube). I filmed the peels using my research scope, which comes with Nomarski optics, which usually introduce interesting colors to unstained transparent materials. When you look at the peel without Nomarski optics, there is much less contrast and, at least on the Zeiss scopes students use (e.g., the one in the video), the peels only have as much color as the light source.
Can I use this method on neem tree leaf?
Probably, but the quality of the peel can suffer if the leaf surface is too waxy or trichome-dense, in which case you can try to remove the wax or hairs.
@@alanharvey5470 Those are some big words dave
Can I use safranin for this
There are techniques that use safranin to stain the cuticle, but since this is an imprint of the cuticle, I'm not sure what benefit the safranin would provide.
If we use glue will it be sucessful to watch stomata under compound microscope ???????
Possibly; some glues definitely work better than others, so your best bet is to try one and see whether it works for you. All the stomatal images in this video were made of peels viewed under a compound microscope.
what you use on the leaf? im not english and i dont understand from listening
Here it's called clear nail polish; in the U.K. they may call it nail varnish. I've tried the clear water-based varnish mentioned in the video (brand Minwax), and actually prefer it to the nail polish.
nail polish
Don’t do science experiments on me!
Nailpolish colour?
Clear nail polish. Some brands, though, seem to damage sensitive leaves. I've had better luck with the water-based varnish briefly mentioned here.
Lov u bro 💯 time 😘😘🥰🥰🥰
May i know the name of the leaf that u are using sir?
Sure! For the main demonstration I used the leaf of a Shumard oak (Quercus shumardii).
@@alanharvey5470 thank you sir
What magnification is this?
4dx
This guy sounds like JP Sears
online school ya get me
Is this slide permanent?
Probably not without further attention, as the peels are taped directly onto the microscope slide. I suspect they can be made quite long-lasting by sealing the edges, e.g., with nail polish.
omg this is kinda scary
Tripophobia 😖
wow just realised this has 100k views
bruh
yes
Lmao
online school is useless
it kinda cringe
This reminds me of will smith in I am legend
dad
That music is copyrighted
rubbish 😒😒we simply can't carry a nailpolish in practical exam
Water-based varnish works just as well, if not better. Don't know if you can bring that to a practical exam, either, but I didn't make the video with practical exams in mind.
You are stupid
U bloody.in ur comment it tells that ur a big######
@Owen Palmer lmao