Quick note from the Bibel Lab 5: protein purifying

So many grad school flashbacks today! I'm so grateful for my training at CSHL in the Leemor Joshua-Tor lab! Especially grateful to the postdoc who taught me, Elad Elkayam!
Much more on all of this in past posts and videos! bit.ly/proteinpurificationtech & bit.ly/proteincleaning ; UA-cam: • Overview of recombinan... & • Protein chromatography
More updates from the Bibel lab: • Quick "lab notes" from...
more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbiochemist.com

[@LugiusEX]

As I current graduate student, I just want to say that I admire your enthusiasm and passion towards showing us important concepts of biochemistry! :)

[@thebumblingbiochemist]

Thanks!

[@JackieLiu0406]

For my protein dialysis bags, I always tight two dead knots at both ends. :)

[@thebumblingbiochemist]

It's always scary no matter how you do it!

[@CryptoKeeper36]

I feel so spoiled having only ever done purification with an AKTA Pure FPLC.

[@lilgeohaley]

30-50 mM Imidazole seems really low for an 8X-HIS elution. Are you sure that’s not non specific binding coming out and your protein is still bound?

[@thebumblingbiochemist]

We realized there was a typo and it was actually ~150-300!

[@samx90]

Very Good Narration of the workflow.

[@thebumblingbiochemist]

Thanks!

[@randomname572]

How pure was your protein after only nickel purification? We always also do a SEC run to polish and put it in the right buffer aswell.

[@thebumblingbiochemist]

Surprisingly pure! We did dialysis since we can't easily do SEC here. See part 2... ua-cam.com/video/Uma0LmM5MSg/v-deo.html