I’ve been trying to do a CAS assay but the method adds the CAS directly to the growth media and very few of my isolates can even grow, I’m going to have to try this overlay method. Funny how I stumbled across this channel at the same time I’m doing many of the same assays. Thanks for sharing
Is O-CAS just qualitative or is there a way to look at absorbance or something to get some numbers for siderophore production? BTW I've learned to never try to quickly cool a glass bottle after autoclaving bc same thing happened to one of my buffers where the bottom broke off and I got imidazole buffer all over my PI 😂😭 I'm sticking to good 'ol newtonian cooling on the bench
The O-CAS is just qualitative, but there are quantitative liquid-based CAS assays. That review article I link to has some more info. Sorry to hear about your bottle breakage! I put them in there all the time. Normally it works fine and is really useful for cooling things evenly (so the bottom doesn't solidify) and keeping things from over-cooling and instead having them ready to pour whenever you are!
that is, you first added the nutrient medium to the plate, it solidified, then you seeded the bacteria. and after what time did you add the mixture with CAS?
I’ve been trying to do a CAS assay but the method adds the CAS directly to the growth media and very few of my isolates can even grow, I’m going to have to try this overlay method. Funny how I stumbled across this channel at the same time I’m doing many of the same assays. Thanks for sharing
Good luck!!
You've given me the inspiration I need to get back to studying! Thanks!
You can do it!
Wow Beautiful , from korea
Thank you! Best wishes from the US!
Is O-CAS just qualitative or is there a way to look at absorbance or something to get some numbers for siderophore production?
BTW I've learned to never try to quickly cool a glass bottle after autoclaving bc same thing happened to one of my buffers where the bottom broke off and I got imidazole buffer all over my PI 😂😭 I'm sticking to good 'ol newtonian cooling on the bench
The O-CAS is just qualitative, but there are quantitative liquid-based CAS assays. That review article I link to has some more info. Sorry to hear about your bottle breakage! I put them in there all the time. Normally it works fine and is really useful for cooling things evenly (so the bottom doesn't solidify) and keeping things from over-cooling and instead having them ready to pour whenever you are!
that is, you first added the nutrient medium to the plate, it solidified, then you seeded the bacteria. and after what time did you add the mixture with CAS?
Correct. We added the CAS several days later.
@@thebumblingbiochemist thank you 🙏