Determination of Unsaponifiable Matter of an Oil or Fat Sample (Part-1)_AOAC 933.08 & IS 548-1

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  • Опубліковано 28 гру 2024

КОМЕНТАРІ • 51

  • @annag3373
    @annag3373 10 місяців тому +1

    Do you know if carrot juices require saponification, too? Do you extract beta-caroten from fruit juices only by centrifugation without saponification?

  • @RadheySingh-bg4ed
    @RadheySingh-bg4ed 9 місяців тому

    Very nice and work helpfull

  • @NamLim-manh
    @NamLim-manh 2 місяці тому

    Could you please provide "how to separate and synthesis stigmasterol from unsaponifiable fraction"

  • @madhusiva1544
    @madhusiva1544 11 днів тому

    We wash extract more than 25 times but still it's not give the colorless reaction to phenolphthalein.what is the solution for this problem?

  • @호우-h4j
    @호우-h4j Рік тому

    what is the purity of the KOH pelltet?

  • @alibhutta2799
    @alibhutta2799 Рік тому

    Pls Guide can I use 0.5M ethanolic KOh instead of 0.5M Aqueous KOh

  • @roshan.Lab-91ok
    @roshan.Lab-91ok Рік тому

    Thanx for upload this vedio

  • @alibhutta2799
    @alibhutta2799 Рік тому

    After 14th wash no clear layer formed at top what will be the reason ?

    • @MicroChemsExperiments
      @MicroChemsExperiments  Рік тому

      Loss of hexan with emulsion during washing the extract.
      Wash it more carefully

  • @beatrizcampelodasilva6536
    @beatrizcampelodasilva6536 2 роки тому

    after the saponification step, my solution turn solid, forming like a gel, when cooled. what may had happen?

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 роки тому +1

      If you face to form gel in the separation funnel then it is called emulsion. To remove emulsion, add ethanol and shake again

  • @purnimapatel3483
    @purnimapatel3483 2 роки тому

    I used petroleum ether for extraction. I got separate layer bt by washing ethanol and distilled water It did not separate what can I do.

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 роки тому

      Ethanol is fully miscible with water. So you will not found separate layer between ethanol and water. During this test, ethanol will be discarded along with the water. Just collect the hexane layer after washing.

  • @alibhutta2799
    @alibhutta2799 Рік тому

    while performing experiment everytime emulsion is formed in which step am doing mistake pls Guide

    • @MicroChemsExperiments
      @MicroChemsExperiments  Рік тому

      Please add extra ethanol to the emulsion and shake. The emulsion will be cleared out

  • @felipebrondani8167
    @felipebrondani8167 Рік тому

    Hi. Can I do with less than 2.5g of oil?

  • @asifashraf6235
    @asifashraf6235 2 роки тому

    Sir i shall be thankful to u kindly send the testing method of fragrace purity

  • @narendarpratapyadav9908
    @narendarpratapyadav9908 2 роки тому

    Hello
    Sir give me some information of NPN check in maize gluten please
    Thanku..

  • @alaminlemon8544
    @alaminlemon8544 4 роки тому

    Please make video 0.1 NAOH solution with Standardization

    • @MicroChemsExperiments
      @MicroChemsExperiments  4 роки тому +1

      Alamin Lemon, we used commercial standard 0.1N NOH solution (ready made) in this test, that's why we didn't standardize our solution. In future, we will make video of a procedure for standardize different solutions including sodium hydroxide.

  • @Wardi_lovers
    @Wardi_lovers 4 роки тому

    Sir please provide us "how to analys sugar percentage in crude"
    I hope you upload the analys video of crude sugar percentage soon.
    Thank you 🙏🏻

  • @pranaysarkar1611
    @pranaysarkar1611 4 роки тому

    Lots of love

  • @Titi-je3ff
    @Titi-je3ff 4 роки тому

    Thanks a lot

  • @harshitakaushik9058
    @harshitakaushik9058 2 роки тому

    According to IS 548, nowhere they have used hexane. Why is this video using hexane?

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 роки тому

      Petroleum ether and hexane are very similar in chemical properties. You can use both

    • @harshitakaushik9058
      @harshitakaushik9058 2 роки тому

      Great, thanks. I'll perform this experiment today and comment here if I have any doubts

    • @MicroChemsExperiments
      @MicroChemsExperiments  2 роки тому

      @@harshitakaushik9058 go for it

  • @manoranjansahoo8586
    @manoranjansahoo8586 Рік тому

    Engine oil test

  • @goda2002
    @goda2002 3 роки тому

    thanks

  • @purnimapatel3483
    @purnimapatel3483 2 роки тому

    Please send me reply as soon as possible, because I m doing this practical now.

  • @samiyainspiration
    @samiyainspiration 3 роки тому

    Thanks a lot

  • @amrishsinghparihaar6529
    @amrishsinghparihaar6529 3 роки тому

    Thanks