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On the website hotshot method is recommended for DNA extraction so does it mean that the hotshot method is good for fresh feathers but not for taxidermy feathers? And can you please share literature of the second method of DNA extraction
Awesome video!
Thanks Nathaniel! Hope you're doing well :)
Can you do PCR with goldfinches as well?
Yes, you could. They belong to the Passeriformes group.
Did u change Mgcl2 concentration
Hi Sangeetha, no we kept to the 7.5mM concentration of MgCl2 in our HOT Firepol Master Mix: bento.bio/product/hot-firepol-blend/
On the website hotshot method is recommended for DNA extraction so does it mean that the hotshot method is good for fresh feathers but not for taxidermy feathers? And can you please share literature of the second method of DNA extraction
Awesome video!
Thanks Nathaniel! Hope you're doing well :)
Can you do PCR with goldfinches as well?
Yes, you could. They belong to the Passeriformes group.
Did u change Mgcl2 concentration
Hi Sangeetha, no we kept to the 7.5mM concentration of MgCl2 in our HOT Firepol Master Mix: bento.bio/product/hot-firepol-blend/