Mystery Tumor Part 2: DiffQuick
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- Опубліковано 29 сер 2024
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Sometimes this part is usually covered by the lab tech (me!). I used to work at a hospital where they would call us in during bone marrow aspirations and lumbar punctures. I would work along side an oncologist and make slides for them bedside.
So cool! Thank you for all you do❤️
It's always a good day when you get a sample with plenty of spicules.
My husband will never forget the feeling of the NP hand drilling for his bone marrow draw😂❤ it takes a special person to do that all day long.
Slides was probably the first thing I learnt in the bio part of lab tech school
I work at the check-in desk so it is cool to see some of the lab tech side of my job. Since I don't work in the actual lab. Just the phlebotomy part where we collect the blood. (our hospital is different then most)
You and so many of your followers are full of great information. Thank you!
So glad you are getting value out of joining this community 😊
Nothing to see here
Read more
@@UTKC_Officer_Smilesit might catch some people but the “read more” thing is normally gray and everyone’s text is normally white unless it’s a username like at the beginning of my sentence.
@Adora_therion_12 everything she posts has needles
Half of her videos are complete shit, she contaminated a scalpel in a recent video and labeled it a life hack
former Lab Tech here. brings back so many memories of blood smear diffs... saw more cancers than Basophils though
Im so sorry💔 Praying that the patients are safe, healthy and well❤
@@julesoxana I'm honestly not aware but I do hope the same as well
Hi! Just curious I'm looking into lab tech, I was wondering what made you change your career?
@@reuey2307 the job market where I am which probably doesn't apply to you. As well as some of the bad management decisions made by hospital administrators which made me feel like I was working at a slaughter house rather than a hospital. Honestly I still love the work, I just hate the environment.
Do you dip multiple samples in the same solution wells? Curious about cross-contamination issues. Why not drop the solutions over the slide?
Vet tech student here- these videos are seriously a lifesaver. Some of my materials for class aren’t as clear as I wish they were, so your channel has done wonders to balance that! Thank you!
Same, I hate histology though
“Blow the cells out with as much force as humanly possible 😊”
*Sneezes directly onto slide*
The first of many problems*
Lol
This made me think of osmosis jones
"We have to use some oil to make it look clearer"
The cell after the oil: 😶🌫️
There should be a coverslip on top of the slide - microscopes are set up expecting to have the light.passing through the slide, sample and coverslip.
It also keeps your lenses clean. I doubt that 100x has been cleaned in it's life. We cleaned microscopes weekly in the microscopy department.
A thick sample will also look fuzzy - it's like a bokeh photo, the depth of focus is very narrow at high magnification.
@@blackhellebore89 Thanks. Exaclty, I hate it when ppl dont take care of shared lab equipment...
Never forget using the least magnification at first. I made the mistake a couple of times in college and I had to get new samples.
Why can't you just back out the magnification and try again?
@@desslou Because I was just learning how to use them (they didn't teach us in school) and disturbing the sample, most likely, you'll skew the results.
Proper microscope usage dictates that you always start at the lowest magnification and NEVER leave your oil immersion lenses in the home position after walking away. This is how we end up with scratched lenses that cost thousands of dollars.
And clean up the oil from the lenses after each use, lest it seep in and make them unusable over time or make a mess all over the scope. My coworkers love to leave oil all over.
because if your stage is in the wrong position you just scraped your sample with the high power lens also
I just graduated as a cytologist! And this will be my job as long as I pass my board exam soon! Love the field and love this video!
I know you passed so congratulations!
We call it immersion oil ! My microbiology teacher taught me!
We usually use cedarwood oil for OIO so I only say cedarwood 🤣
All I could think was “note the gram positive cocci in strains and that it probably contamination” 😂
Omg u sound like Lisa Nguyen! Also this looks so cool 🤩 keep up the good work!
Medschool told us to do it with specific timing like "dip it in the blue dye, keep it still for 30 second, then dilute"
Do professionals really do that? Or is it just standard procedure?
Im a vet tech and at different clinics Ive been trained on different methods but usually they say to hold it in each solution for about 15 seconds or dip in and hold for 2 secs at least 10 times. All the methods I’ve been shown seem to pick up the stain well so we do it but usually not for 30 secs and I dont think it being still or dipped really matters they’re all just methods to make sure its in the solution long enough
Not really a standard, but it gives the stain more time to get into the specimen on that slide.
Wouldn't bits of the specimen contaminate the bottles by dipping them in?
@@ShamelessFNGRLexactly this - you wouldn’t use the same bottle for the next slide, it’s bad practice.
Biomedical scientist here, thousands of different staining techniques exist with varied needs. I'd always recommend adhering to time recommendations, should work out better
I find it interesting that you dip the sample into the staining solution, we were always taught to place a large drop on one side an absorbent swab on the other, pulling the dye across with osmosis, I can see how that method would be faster, though how do you prevent contamination of the staining solution?
I was wondering about contamination also.
That's a cool technique
I've done it like this before, those containers don't necessarily have to be the original ones.
@@adriasalarich that makes sense but what about contamination between the slides your dipping, or would you typically stick to slides relating to the same test,sample and patient.
@@The_Cloth_Surgeon as far as I know you'd use the same containers for slides from the same patient/tissue. I've also seen in other comments that cross contamination isn't really an issue since the cells that might remain in the containers won't really get fixed on the slide, and they'll be easily washed away between every step.
Thanks for the tutorial on how to contaminate your dyes 😊
TYSM these refreshers really help!!!
You’re welcome!
So that's how it's done! Thank you!
You’re very welcome! 😊
I’ve looked at cells in my microscope during science class the other day
Now this is what real content is!!! 👏
In highschool when we were learning how o make slides they sid not cover this, and now i know why i could never figure out why it was so hard to see all the things they wanted us to see. This is so fascinating to see.
It also helps to put on a cover slide, which is acually calculated into the optics of biological microscope objectives. If its not there or the incorect thickness or refractive index, the image will not be flat, clear and fully in focus over the full field of view.
Also, adjusting the illumination system is vital to proper contrast and illumination. Look up critical illumination and koeler illumination.
GOD DAMN I GOT EAR BLASTED BY THE NEXT VIDEO BECAUSE YOURE SO EFFING QUIET
I actually did this in my microbiology lab,it was so fun and cool to see the results
I've learned more about how to do this than all my years in science class.
This is incredible, im in awe!
Thanks, I’ve been trying to do this all day, this perfectly explained everything.
i miss my microbiology class :')
I love microscopy, I got into it for Psilocybin mushroom research.
You know, I’ve never seen anybody use a technique quite like that before. Something nice to think about.
Glad you found it interesting! 😃
My favorite is a bunch of slides with the name only. Like OK, but which freaking tumor?!
The mitochondria is the powerhouse of the cell
Dexter would disagree... never label the slides!! 😂
I have a degree in Biochemistry and LOVE watching these
Instructions unclear... I now have a needle inside my hand and several plastic syringe shards
That's so different from how I was taught in Micro! So cool to see this done
Thanks for this!
As a phycisist the last bit was interesting to see how optics is used
It's so crazy because when I took microbiology in college, I asked my professor if I could look at my blood under the microscope and she said no. little did I know in bio 224 everyone has to prick their finger to look at their blood under the microscope. and I have sickle cell disease and it was so cool. Being able to finally see my own blood for myself.
in microbiology the staining method was a lot more strict xd
The way you colored the slide made me anxious
Omg. Science is so fking cool!
What chemicals are used in the staining? And how does the distilled water not wash the cells off of the slide?
@@Vetventures ohhhh okay, thank you! I tried making a blood smear at home one time with some methylene blue but I didn't understand the process and had only stained some cheek cells with a slide cover before, so it was super interesting to learn another side of making slides!
@@vitalricecakes8274 hellu,
So for your blood slide you need two slides, a damp paper towel, a dry paper towel and patience.
It's certainly not easy to get a good one but here's how I do it.
1. You are looking for a result with no frayed ends. So it shouldn't look like it was painted with a brush. My teacher said "look for a tongue or a flag" (round or angled end). Little fray can be ignored. (Trust me you won't get a clean edge)
2. Take 1-3 little drops of blood (we use capillaries for application), dependent on the consistency, and place it on one end of the slide in the middle with some space to the edge
3. Take your second slide. Don't hesitate much, don't think too hard about it. The longer you wait the more it will dry. Aim for 45° angle. But do play with the angle of you feel like it's not taking the blood far enough. Again this depends on the consistency.
4. In a swinging motion bring the slide to the drop, then in the opposite direction, dragging the blood with you. If you are doing it correctly the blood will spread vertically on the edge of the second slide.
You can imagine it like a boat swing. So start from the top, gently swing in and with the exact same motion you came in, swing back to the top.
It is important that you do not go over the edge of the slide with the blood. Stop at the right time.
Try to make one smooth motion, you don't want to have a barcode on your slide do you?
Ideally use EDTA blood, but if you use blood from your finger: wipe the first drop away and do not use it. It is full of water and cells you don't want to clutter your vision. The second drop is the one you want to look at.
Why the wet towel? To dap the second slide in to clean it in between tries. Small dried blood could make some "scratches" into your smear.
The dry one is so you dry it afterwards.
You can hold the second slide how you want but I find thin/thin better then thin/long.
IMPORTANT: before you stain your slide, let it dry Completely!
We placed paper towels on ours and let it sid for 15 minutes in practice (after it was dry of course) just to make sure.
I will edit this post to write you my times and step by step guide for the coloring process.
For looking under a microscope: search the monolayer plain.
This is where every cell lies on its own and can be seen and evaluated. In the red blood cells you need to see (in healthy blood) the concave shape, so a "white" centre. If this disappears, you are getting into the "to squished" plain.
Also you will need to look through it with 100x Objektiv and oil.
Or at least 60x
Of course you can see the cells in smaller views but not the pretty details.
If you are interested see if you can find a "Pappenheim" coloring process.
Edit: Sadly I don't have a step by step for mythelenblue, but if I had to estimate.... Two minutes should do it, then gently dip it in one glass of distilled water, and then in a second one
@@sleeples7852wow, more great advice. Thank you! My son is doing homeschooling this year, so all the science is on me. lol. I appreciate all the amazing information. 🫶🏻
@@sleeples7852👏🏽👏🏽👏🏽❤️❤️❤️
@@sleeples7852what a reservoir of information. Thank you
My mom does this! And she also does the FNA
“As humanely as possible”
I broke my right salivary gland and was is in hospital for operation, it went very well
Isnt the stain contaminated after dipping like that?
the cells are dead and stuck to the slide once it dries. as long as they are "fixed" it should be okay
And the oil thing. Idk why she said anything about it. If ur microscope uses oil, you know about it!
@@Wtfincit's called oil immersion lens of the microscope( the eyepiece usually has a scanner,10x,40x and 100x lens;oil immersion lens is 100x), to get a clear image at 100x magnification you use oil. My med school uses cedar wood oil.
@@Wtfinc because this is an educational video for anyone and everyone? i didnt know about using oil because i dont do this stuff but still find it interesting to watch lol
The sample is usually attached ti the slide w thick substances like ear wax we’ll run the slide over a flame for a bit before staining. But it does contaminate the solution after awhile so you have to replace the stain every month or so
This is all well and good, but it still doesn't change the fact that the mitochondria is the powerhouse of the cell.
Bro can see plankton right there.
💀
Literally only followed the story to figure out what the blob was till I realized I think this just a demonstration 😭😭😭
Gram staining! Nice
Wow, this looks so much better than the way they were teaching me in college
I usually rinse in between dipping so you don't contaminate your stain.
Instructions unclear, hypodermic needle stuck in wall
That's exactly what we did when we did our graduation, masters is boring though
I will suggest this channel to my gf when she will be in medical University
BIOLOGY CLASS TIME 🗣️🗣️🗣️🗣️
Rinse the back!! You forgot to mention to rinse the BACK!! You can still rinse your sample off if not careful!
U taught me better than my bio teachers
At last a proper microscope video
Look at the bacteria in the cells! 😮
Damn, light micrsoscopy is so cool! I’m in electron microscopy, and the whole staining and washing process is so tedious!
I love that you arent using gloves
Histotech here, I have a lot of respect for cytotechs because I find bodily fluids much harder to work with than tissue.
We need a part three
My high school bio teacher stabbed a bottle of cell dye so hard it exploded on the both of us. Took a lot of rubbing alcohol and hairspray, plus a few times in the wash, but one of my favorite hoodies is still going strong today. CELL DYE IS NO JOKE!
My mom does this !!! Senior Lab Tech 🎉❤
Thanks 😊 Subscribe & hit the notification button👍 👌 😀
Scientists flourishing here 😂🎉
Reminds me of my microbiology class
Damn, that hand writing... looks like a toddler's hand writing
If I had the stuff for this I'd do this for fun
Microscope service technician here. Always use immersion oil not mineral oil
She forgot to mention to quickly bring the slide back and forth over a flame to make the cells stick to the slide once spreading them. This helps the cells to stay on the slide when preforming dyes or other test on them. Also for infections diseases like bacterial it is done to kill them so they don’t contaminate them, and for them to aerosolize and spread and infect you. Lucky cancer cells are not contagious but still better to heat stick them to the slide so you don’t lose them off the slide, or if you need them to be alive and mobile better to put the stain on them with a dropper or pipette rather than dunking them into the stain.
it sounds like minecraft splashing potions when she dips the glass slide in the solution
love how the yellow blob looks like everything but a yellow blob
Just wait till you meet centaurs
Cool to see this process. I had a stage 3 testicular cancer adventure and we didnt know until biopsying a tumor in my neck!
Yo, where are the gloves and pipettes? Dipping a slide into the stain solution cross contaminates like crazy!
Dif-quik stain is always used this way. At my animal hospital we have one set for "dirty" samples like ear and skin cytology and one for "clean" samples like FNAs and blood smears etc.
There are no bacterial cultures or anything going on in this process so contamination is not that much of an issue. Any cells floating around in the stain will not stick to the slide and will be rinsed away in the last step. Stains are changed regularly so they don't get too dirty.
@@joeyk9441at your vet clinic** let's not get ahead of ourselves here, y'all are barely doctors
The way u stain is so much easier and much less time consuming that what we learned at uni 😭
Finally! I was wondering why those were colored like that, I had a hunch they weren't actually shaded like.
Bold of you to assume I have a microscope
So THATS why cancer images are pink/purple! I didnt know
The narration is like hey here is a quick and easy tumor reveal process. Comment below for your fav hack lol
Cross contamination in all of the stain jars
I love videos like this, but why dont you use pipettes? Seeing the slide being dipped into the dyes just seems unclean and contaminates the dyes
Edit: typo
That microscope looks… professional
I have done this in my first semester as a med student in physiology lab and we saw our rbcs and wbcs . Lol i was even asked about the emersion oil used for 100x lens she is talking about in viva
Reminds me of the IHCs I had to carry out for my masters dissertation project. Love how you explained everything so clearly. Wish I had seen your video earlier as no one explained to me how to use the microscope, had to figure that out for myself😅
Damn bro oil really is the most useful thing ever
I’m not this kind of learner. I need you to write down the steps 😂😂😂
Is this why HELA cells ended up literally everywhere accidently?
I did this in biology this year
“…spread the cells apart…” hooo mama, one of those Friday nights I suppose. A hootenanny of legs, yessir. A jamboree with a stick and a drum and then you beat it. A dance of fireflies, sunset on the Vatican, hem-hawing the hedgehog, threading the ole needle. Two bits in a bundle. Hot dog. Me n the missus “in the workshop”. Midnight cacophony. Tuning the gearbox, stirring the stew. Lockpicking. Heck, kids these days have even gone so far as “the Monster Mash” and the “Horizontal Mamba”. How unsubtle. I much prefer “the reclined jumble.”
Bizarre innuendo aside, this is cool look into how this stuff works
For that tint we had a machine that does it automatically, but I have also done that by hand
H&E perhaps?
Mitochondria is the powerhouse of the cell
I could be blind and dumb but that's not a yellow blob
Ok this is really cool😮
Contaminating the dyes...
Ngl I have no idea what you’re talking about but it seems cool and helpful so new sub!
This is Middle School 8th grade Biology. Basics. Preparing your slides.
Ahh microbiology flash back 😂
О, неужели героин?
So what did we find?
Does the fish have cancer or not?
Or was it just faking it's tumor?
I have no context for this and I'm so confused, why are we doing a diy microscope slide asmr tutorial