Streak Plate Technique for The Isolation of Pure Culture - Microbiology - Alpha Genomics

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#streak #plate #isolation #pure #alphagenomics
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The streak plate technique is used for the isolation into a pure culture of the organisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in such a way that it “thins out” the bacteria. Some individual bacterial cells are separated and well-spaced from each other.
As the original sample is diluted by streaking it over successive quadrants, the number of organisms decreases. Usually, by the third or fourth quadrant, only a few organisms are transferred which will give discrete colony forming units (CFUs).
Principle of Streaking
The sample/inoculum is diluted by streaking it across the surface of the agar plate. While streaking in successive areas of the plate, the inoculum is diluted to the point where there is only one bacterial cell deposited every few millimeters on the surface of the agar plate. When these lone bacterial cells divide and give rise to thousands and thousands of new bacterial cells, an isolated colony is formed. Pure cultures can be obtained by picking well-isolated colonies and re-streaking these on fresh agar plates.
Materials required:
A source of bacteria (stock culture, previously streaked agar plate, or any other inoculum)
Inoculation loop
A striker/lighter
Bunsen burner
Lysol (10%v/v)
Agar plate (nutrient agar or any other agar medium)
Paper towels
Related Tag:
#Streak
#PureCulture
#Microbiology

[@MuhammadRamzan-rl4gd]

Well explained, thanks 👍

[@AhmedHassan-wk2ru]

thank you

[@theeeriepolymath539]

label the petri dish instead of the lid, because lids can be lost or exchanged.
keep the plate partially covered with the lid when streaking and entirely covered when heating the loop .
ap ne plate ko bohat zyada der uncovered rakha tha..contamination ho jaati hai

[@user-yq6xz7tn5g]

can anyway please tell me where i can find the four flame method for streaking

[@afnankhanytshorts3030]

Well explanation mam

[@MuhammadRamzan-rl4gd]

Continue it Plz

[@user-yq6xz7tn5g]

dont open the lid completly it can cotame your agar plate use 3 finger metod to open the agar plate

[@AmbreenNazButtAmber-se6fl]

Well explaun

[@mairaashraf2386]

Iske bad bacerial colony ki vedio ni Bnai kia mam?

[@divya821]

Urine loop ka size kitna hota hai

[@tusharkoujalagi9926]

Where are the gloves? Aseptic technique? Labeling should be done on base of the plate & not on lid.
Wrong technique

[@mamoonasardar4148]

Ma'am... can we make 4 quadrants streaking ?

[@divya821]

Kon sa bacteria loop use hoga urine m

[@faisalsadiq7698]

very helpful... god bless you