Sad to see this experiment hasn’t been taken any further in 3 years. This genuinely made me feel interested in science for the first time in my short 20 year life.
@@thethoughtemporiumI would think that this would get funding fairly easily due to how strong spider silk is and the amazing utility we could get out of growable high strength building materials, and something I just thought of while watching the video explaining the structure, spider silk is a fabricy version of reinforced concrete.
Dude, I cant believe you're making this technology open source. I've been reading about the possibilities of harnessing spider silk since I was a dork in middle school, and remember thinking the guys making the spider goats were cool as hell. I really hope you manage to extract and polymerize silk from this.
Because he's one of the good guys, not subverting science for the highest bidder. He's one of those scientists that need crowdfunding the most, to keep him free of the likes of bigCorpo who'd put a life patent latch on it and shut it for good.
Right? I remember watching Ripley's believe it or not as a child and one of the guys featured there had made a really durable, strong suit out of bunch of materials including spider silk he milked out of actual spiders and since then I wondered about the possibilities of creating some super hero costume out of spider silk. It's truly wonderful that people are coming together to make education easily accesible and likely creating future scientists.
Instead of wet spinning, try electrospinning. It should give you aligned fibers instead of randomly oriented ones from wet spinning. You should be able to cut a section and twist the web fibers into actual thread that are theoretically aligned so you get better load sharing on each fiber.
30:15 I love that I live in the future. The idea that I share the world with a UA-cam-vectored, open-source, melt-printable, github-published bio-kevlar makes me happier as an adult than ice-cream did as a child. Thank you.
Makar Ismaile Adnan He’s happy that the creator of this video is so transparent with his process and so willing to share his results. Plus, the fact that this content is distributed for free.
This is really “the future” it’s the transitional period between the past and the future. Right now everything cutting edge is being discovered and all these cool products will most likely be widely available by the time we’re all dead or really old.
Guy who invented penicillin: "Patent? That's absurd. You wouldn't patent the sun, would you?" Biotech firms: "Spider silk? Oh that was us, we invented that. Ours".
I have absolutely zero biochemistry background, but I somehow feel like I understood most of what you explained in this here video. Amazing work, and as a Spider-Man fan, I'm eager to know more about the possibilities of IRL webshooters
This part you probably already knew, but using IRL web shooters with absolutely be one of the most dangerous things you've ever done or did anyone could realistically ever do, but spider silk binded with carbon nano tubes. Could absolutely if made correctly hold the weight of multiple humans, I don't think you're doing Oh that the web shooter could do in a little canister. That's the size of your pinky but in especially designed flat pack tank that goes over your stomach, now we're talking. Because of how easy it would be to vary the properties of the silk when you produce it, depending on which particular strand of genes you use, everything you see Spider-Man doing with webs would be at least theoretically possible to do with at least a version of what is demonstrated in this video
Joel Creates I wanna say same, _but..._ I don't know calculus yet (among many _many_ other things that would likely leave me a bumbling fool in most any *_real_* lab) 😞.
I had a professor in college. This is the kind of project he was barking about in his often encouraged tangents during biopolymer class. Your project is like a customized and detailed exploration that goes well beyond what he could elaborate on in class. Thank you for peaking my interest!
@@thethoughtemporium Maybe Ben could X-ray diffract image them, or run them through a mass-spec, or the Electron mic, or ALL THREE! amazing work, there should be a "UA-cam science medal" or something.
i think we are on the cusp of a revolution ... who needs elsevier , springer or iEEE these dudes together with a couple other sci-tubers are doing more cutting edge research than many well funded snob colleges.
@@thethoughtemporium Growing fibers is cool - But I would reccoment that you try mixing some into a Kombucha brew and make a SCOBY - you'll get nanocellulose tied to fibroin... it might be revolutionarily strong. Use weak/white tea if possible, and you can actually just use mineral supplements in sugar water for a "cleaner" SCOBY. Also, that way, the yeast/bacteria make the sheet for you, and you don't have to worry about the fiber weaving process.
"the plasmid has been shared on my github repo for that want to mess around with it" imagine saying that sentence to someone in genetic engineering 25 years ago
As fellow You Tubers we continue to try to improve our content aware of the friendly "competition". You keep raising the bar...Damn Excellent video, keep it up!
So what is the current status of this project? Have you scaled up the process and/or have enough of a sample to play around with for a video demonstration? Would be incredibly interested in obtaining a sample of the yeast for a related personal project (involving kombucha SCOBY bacteria) if at all possible.
As a molecular biologist, the explanations in this video are better than most of my uni courses. Difficult principles made simple. Explaining something so that someone uninitiated can understand is a gift. Kudos for the job well done, your countless hours spent on this project were not in vain, sir!
My mind is blown. I knew bioengineering was a field that existed, but had never *really* thought about it. When I watch a video on chemistry or physics, I usually get most of it. I understand enough about chemistry to usually follow along with whatever video I'm watching, even if it goes to the slightly more technical side of things. *This* video was 33 minutes of me going "wat." Okay, making yeast produce things. I've heard of that. Wait, you can just write DNA like you're a programmer? Wait, there are companies that will print DNA projects for you? This is common enough that a business can be made for it? Wait, you're taking a bunch of different genes and randomizing them and somehow you get the properties you want? Oh, silk sort of doubles back on itself for added strength; I get th- Wait, you can just add properties to it like it's a new spell in a video game? Wait... Suffice it to say, I think the remainder of my day is going to be spent learning as much as I can about this. Fascinating is too weak a word.
Man im so happy for you! I watched the first video a couple years ago and I was very anticipating the final and finished product! Congratulations on figuring it out and getting it to work!
I can't express how much I love that you document all this content for us to watch. Science in all fields can be so interesting, but it's so insular and stuck in the world of papers and journals that the average citizen will never hear about all the cool shit going on. You and many other science youtubers are really doing a great service.
You call fax I mean we are all forced so deep into the thought that science is all boring stuff on paper but no it's fun aswell like artificial meat or aerogel and theories like our universe isn't finite and it's a loop from one point to another or that aerogel can be used in rockets as insulators.
I remember how in the action lab they mentioned that the structural strength of steel regarding tensile strength is in the gigapascals. Even specialized steel it's not like it's 10,000 times which spider silk can handle or whatever. Who knows how many creatures have a shell made out of nacre oh, it's tensile strength depending on the species is 170 gigapascals, even if one gigapascal is 2 megapascals this is still a whole lot more than steel. The trouble is this bad boy takes 170 gigapascals to bend, or is that bend it to a point that it will no longer Bend back. Either way that is a whole lot. I did the conversion for both of those, 170 gigapascals is more than 24 million pounds per square inch, and 70 is more than ten million. I did the conversion for both, I checked the conversion multiple times for the tensile strength. I'm pretty sure that I did the 70 kg pascals correctly given how much of it was copying and pasting, and using an online calculator. This is far beyond Steel. Now this is so strong because of the way that it is aligned, according to Google it's like more than 300 or something times stronger than the material it's made out of. So imagine graphene was in the structure that this material is in. And I'm not talking about the structure of which its atoms are aligned. This bad boy is made in a structure of pretty much interlocking tiles, so imagine this was what was going on with a flat thing of the functionally two-dimensional graphene oh, that bad boy would be incredible however graphene is very flexible so this might defeat the purpose. However something tells me that if the graphene were to be bio mineralized to be interwoven with the nacre be beneficial.
ShamelessDuck like you can print DNA?! that is kinda crazy, just humans writing their very own DNA designed specifically to hijack in this example yeast. I searched DNA printing on google and you can apparently make custom creatures with it, which is insane
@@tormodhag6824 I dream of future when we will use GMOs legally in our everyday life without it being marketed as "non-GMO" and without this whole stigma around it.
Ikr And I love how the God-Tier UA-camrs all know each other XD Applied-"I built a scanning electron microscope from scratch in my shop"-Science and Tech-"I made a nanostructure imprinted chocolate bar diffraction grating"-Ingredients both commented here alongside us! XD
Rewatching this video a second time and chuckled thinking about how people used to think everything was made of air, water, fire, and earth, and here you are doing this sorcery
Well Toby McGuire his webs were natural Andrew Garfields were stolen from oscorp and Tom Holland I don’t know but I’m pretty sure in the comics (I’ve never read them so this might be wrong) the spider bite gave him the formula composition and he was able to buy the products to make it
I'm an art student but I'm sitting here, 5:15 pm, watching a man make spider's silk out of yeast. This is the coolest shit I've seen all day dude. Can't wait to see what you come up with next. Love the mad science bro.
Huh I've been learning that sht to get marks from the 9th Grade and now I can use that knowledge to brag to a random guy on the internet because I know it and he doesn't [I now realize that's all that information is every going to be useful for]
I know right, it seems that there would be some legal trouble with whoever made the patent and the original genetic designer of the animals, or would the actual spider be the patent holder of the silk since it originally created it, either way it doesn't exactly seem legal.
4 mins in “what is he even talking about” 20 mins in “of course the pPCI9k needs to be linearized with a restriction enzyme! You can’t have those DNA rings, duh!!!”
I interpreted this as an informal video-essay type of report on his findings, truly fascinating, yet extremely confusing to people without bio-majors, like me ;)
"I've become quite competent at designing DNA from scratch" "and just have a company PRINT the DNA" Jesus christ watching your videos always makes me awe at the times we live in...
@@0th_Law Tarantula DNA? They use their hair to detect changes in the air. Spidey Sense was meant more to represent an invisible web of sorts, though I've also seen it as representing the 8 eyes spiders have though they actually have really bad eyesight. But by coincidence Tarantulas already have a spidey sense going for them.
It’s crazy going from “wow that’s pretty cool, no idea how you did that, but it’s cool” when watching science UA-cam to “huh, I did something similar in class last week, cool!”
Spidey yeast Spidey yeast Produces proteins a spider can Makes a fiber any size, matches kevlar any time Look out here comes the spider yeast Is it strong? Listen bud,he's got enhanced gene strands. Can it be made into a thread? Take a look over head. Hey there goes the spider yeast!
I’m actually amazed at how much of your research I understood as a freshman biochem major. Stuff like this makes me hopeful that I could potentially make a career out of genetics because before college, I really doubted my ability to be successful in the field
Well if you want to make something like Godzilla be realistic then try using biomineralization to put nacre in its bones and scales oh, you probably want pockets of stuff like methane in there, methane is a gas produced by living things the most common example that not many people might necessarily know has it in there is when you fart. But farting is made by the methane or swallowed air, by making it have nacre the bones would take literally millions of pounds to not only Pull-A-Part but also Bend, or is that bend it to a point that it will no longer be able to bend back to its original shape. The tensile strength is 170 gigapascal, there are 70 for its strength for the bending thing. 170 gigapascals after I checked my conversion this correctly comes out to more than 24 million pounds per square inch, even if you use the metric system that is obviously a very high amount. The action lab which is a another Dependable source of science information said that steel is generally in the megapascal range, it takes a lot of megapascals to make a single gigapascal. And if its skin is made out of graphene, or whatever term is used for the soft flexible stuff that is what is responsible for holding the scales together on the skin of a snake or lizard. That bad boy would be very durable, for context just some graphene that is the size of Saran Wrap would require Force equivalent to a fully grown male African elephant balancing on a pencil. Not to mention that the Wonder material is also a very effective superconductor so you could probably use this to get rid of the issue of the Godzilla that is more than 100 m tall taking more than a second for anyting to register in its brain because the electricity take so long to move through the body. This graphene could eliminate that issue oh, so we could react without needing to be 15 M tall at maximum. And if it's hunched over like your average T-rex or Utahraptor this could help with blood flow at such a gargantuan scale. And if you argue that it doesn't have enough energy from the atmosphere to oxygenate it, there is an organism that can survive without any oxygen, also considering out at our degraded can literally live for multiple days in the vacuum of space. Something tells me there's probably the required already existing genetics somewhere in the world to let something survive at such a gargantuan scale. But maybe not after all a hundred meters is a very high number for the size of an organism on Earth.
It's actually decently intuitive. If the yeast produces a protein with the ability to bind graphene, then the graphene oxide will naturally coagulate around the proteins, and therefore the yeast, because it is being bound to the proteins.
@@TheIdiotFr0102 If you want, I can clarify stuff for you; just tell me what doesn't make sense. (This is assuming that you mean that what I wrote didn't make sense. If that isn't the case, then never mind.)
@@arrowsaurus7561 You're going to have to be more specific than that. Otherwise, the answer will probably just be something like "Cell Signaling" or "Conformational Changes"
Been following your progress on this for so long. It's awesome to hear you're over that major hurdle! I look forward to hearing how the rest of your experiments go!
Just leaving this out here: A silk/graphene composite generating yeast that survives high salinity would be prime candidate for regenerating tidal turbines
Lol I was in 7th grade and the 2002 Spider-Man came out. My friend and I thought we could make web shooters from aluminum cans and webbing from kitchen spices and cleaners.
Ive been tryna figuring out how to be spider man for the past 3 yrs lol, and I DIDN’T RUN A SINGLE TEST BECAUSE 12 YR OLDS CAN’T GET A HOLD OF NYLON 6, 10 SILICONE AND ADHESIVE GEL. I ALSO WANTED TO ADD HIS SPIDER SILK LIQUID THING BUT I’M TO “UN-INTELLIGENT” TO MAKE IT. AND DON’T ASK WHY I’M YELLING BECAUSE IDK
@@kiriki4558 I was referring to a Beer-CAN full of silk. (assuming you are replying to my comment that is) The practical applications are a no brainer. 10 years ago i would have shelved this under sci-fi, jet here you are actually doing it. btw, the biomineralisation feature sounds amazing. I cant even imagine what properties spider silk bonded to carbon nanotubes would have, but i'm sure it would get you out of a sticky situation, or create them for that matter.
I literally wrote about you in my university application. You're the reason I'm going to university. Thank you for creating videos and making them high quality and interesting!
I think you did a really good job on the visual side of this video, the diagrams, infographics, tables etc. As a graphic designer I was impressed. Awesome video.
dude you are insane, an insane genius, instead of modifying an organism, making a new spider, we get spider beer, spider beer concrete, spider beer kevlar, spider beer plastics. man, respect
I honestly didn't understand what you were talking about. But I was intrigued the entire time. I didn't even know it was possible to PRINT DNA. This is some crazy stuff
@@SomethingSmellsMichy HAHAHAHAHHAHAH Spider Lawyer, Spider Lawyer, does whatever a lawyer does, does he defend? Yes he does, he can also climb the laws, watch out here comes Spider Lawyer!
I’m 15 and I don’t understand most of this but this has inspired me to learn more about biology and also I’ve designed my own web shooters if I could find a way to get the silk liquid.
The first entity to isolate a particular gene gets to file a patent on it under the current biotech regs. Leads to some very undesirable possibilities. It's one reason to be suspicious of those 'we'll analyze your DNA to determine your ancestry/risks' things, because it's potentially a mechanism for someone to gain a monopoly on all commercial use of one of your genes for the next few decades.
@@eichenbrain6170 Twenty years after a corporation had the opportunity to raise the price of whatever treatment it may have turned out to be useful for to the point that thousands of potential beneficiaries went past the point of no return without ever having the capability to pay for the treatment.
Oml I am learning this in my AP Bio class as we speak: gene expression. Seeing it used in a real world scenario and having you explain this made my life so much easier! Thank you, and continue doing cool stuff.
I'd recommend learning the basics of python or another simple scripting language. Your method of random generation of genes is very cool, but a script could be doing all that copying/pasting for you and churn out genes as fast as you can ask for them. You can also have the script do Longest Common Sub-sequence analysis to ensure there's no large repeating blocks that could mess up printing (I assume the synthesis companies do something like this on their end when deciding which orders to reject). Also, your videos have taught me more about practical genetic engineering than years of school ever did. Good shit 👍
I met you at the end of last winter and I remember you mentioning that you had done a lot of progress on spider silk production. Glad to see it all worked out. The video's really informative, too. I'll try to visit Foulab again after this whole pandemic ends.
This is literally the forefront of today's science. I don't believe anyone has come this close. Most researchers: OK, so we grew the spider silk, now how do we add additional elements to it?... Justin: screw it, let's add a gene that binds Calcium, Silver, and Graphene for good measure.
mad scientists in movies: I will take over the world
mad scientists in real life: I will make beer out of spiders
Technically making spiders out of beer.
@@greenaum technically making something not very beer-like out of a yeast variant with spider DNA spliced into it
We have more realistic goals, then?
@@Butter-Milk who’s we you aren’t a mad scientist
mado scientisto in animes: El Psy Congroo.
dude...
X2
love your videos
Anton came out of the Solar System to check out this amazingness!!!
hello wonderful person
LOL
me laying in bed eating cheetos: ah yes of course biomineralization peptides
This is basically me trying to understand how kars survived falling into lava, but with *Spiders*
Hell yea😂😂😂
I dont even understand algebra let alone this. But the idea is interesting enough for me.
I saw a post online about the iron snail a couple of days ago, so I'm basically an expert
@@v1298 yes xD
Sad to see this experiment hasn’t been taken any further in 3 years. This genuinely made me feel interested in science for the first time in my short 20 year life.
It's being worked on, but we're trying to commercialize it so can't share updates yet
@@thethoughtemporiumthanks, I was wondering why it wasn’t being continued
This had made so damn happy. I thought this project was long forgotten
@@thethoughtemporiumI would think that this would get funding fairly easily due to how strong spider silk is and the amazing utility we could get out of growable high strength building materials, and something I just thought of while watching the video explaining the structure, spider silk is a fabricy version of reinforced concrete.
@@thethoughtemporiumlike a suburban superhero???
Dude, I cant believe you're making this technology open source. I've been reading about the possibilities of harnessing spider silk since I was a dork in middle school, and remember thinking the guys making the spider goats were cool as hell. I really hope you manage to extract and polymerize silk from this.
Because he's one of the good guys, not subverting science for the highest bidder. He's one of those scientists that need crowdfunding the most, to keep him free of the likes of bigCorpo who'd put a life patent latch on it and shut it for good.
Haha yeah it's awesome, right? 🍄
Right? I remember watching Ripley's believe it or not as a child and one of the guys featured there had made a really durable, strong suit out of bunch of materials including spider silk he milked out of actual spiders and since then I wondered about the possibilities of creating some super hero costume out of spider silk. It's truly wonderful that people are coming together to make education easily accesible and likely creating future scientists.
Same here.
Even though spider sill is a real good material it also get most of its strength through how the spider twists the silk together
No wonder Tony Stark looked so impressed when Peter said he manufactured his webbing all by himself.
Yes
Yes
Yes
Yes
Yes
Me, not understanding anything but still enjoying it none the less: "Ah, shit, they can't print repetitive sequences, what a bummer dude"
😭
Yup I'm a total Chad ,don't understand shit but it's cool
@@hardanalljr.3138 Are you build as a Chad as well? Cause, cool.
Instead of wet spinning, try electrospinning. It should give you aligned fibers instead of randomly oriented ones from wet spinning. You should be able to cut a section and twist the web fibers into actual thread that are theoretically aligned so you get better load sharing on each fiber.
Instant thumbs up. Been waiting for this one.
Me too. Great videos from the both of you.
Are you okay
This comment prompted me to give the video an instant thumbs up
I will combine Yeast and Starlite and rule the World!
Now he just needs little wrist siphons.
30:15 I love that I live in the future. The idea that I share the world with a UA-cam-vectored, open-source, melt-printable, github-published bio-kevlar makes me happier as an adult than ice-cream did as a child. Thank you.
I really don't get what you're saying, but I'm happy that someone's happy in this world
Makar Ismaile Adnan
He’s happy that the creator of this video is so transparent with his process and so willing to share his results. Plus, the fact that this content is distributed for free.
This is really “the future” it’s the transitional period between the past and the future. Right now everything cutting edge is being discovered and all these cool products will most likely be widely available by the time we’re all dead or really old.
Is it just me or is it crazy that they can Patten certain animal genes, that's absolutely some dystopian stuff right there.
Guy who invented penicillin: "Patent? That's absurd. You wouldn't patent the sun, would you?"
Biotech firms: "Spider silk? Oh that was us, we invented that. Ours".
Capitalism go brrrrr
If someone could patent the sun they would
Let's not encourage them
@@Hoshimaru57 its called nuclear fusion!
Can someone explain how anyone could hold a patent to a gene found in spiders to begin with?
I have absolutely zero biochemistry background, but I somehow feel like I understood most of what you explained in this here video. Amazing work, and as a Spider-Man fan, I'm eager to know more about the possibilities of IRL webshooters
Don’t worry once I have money I’ll make them
This part you probably already knew, but using IRL web shooters with absolutely be one of the most dangerous things you've ever done or did anyone could realistically ever do, but spider silk binded with carbon nano tubes. Could absolutely if made correctly hold the weight of multiple humans, I don't think you're doing Oh that the web shooter could do in a little canister. That's the size of your pinky but in especially designed flat pack tank that goes over your stomach, now we're talking.
Because of how easy it would be to vary the properties of the silk when you produce it, depending on which particular strand of genes you use, everything you see Spider-Man doing with webs would be at least theoretically possible to do with at least a version of what is demonstrated in this video
Arachnologists for decades: "If only we could produce spider silk"
Thought Emporium: "hold my spider beer."
Now I want a “hold my spider beer” shirt.
Hold my Southern Black-Widow in Ethanol*
Ｏ－Ｏ ＷＨＡＴ ＴＨＥ ＦＵＣＫ
I wonder what spider beer taste like
Spider Cider - Made with real spiders
"I've become quite competent at designing dna from scratch"
Why does this sound so menacing.
But dude, youre awesome
because of prions
I mean he's kinda playing God so yes it is menacing
You WHAT
i can now become spiderman
*He's becoming a Spider-Man villain*
I'll be following the development closely since, you know, I'm something of a scientist myself...
Lmao when is the Hot Glue Web shooter swinging video coming up?
Joel Creates
I wanna say same, _but..._ I don't know calculus yet (among many _many_ other things that would likely leave me a bumbling fool in most any *_real_* lab) 😞.
@@ivoryas1696 I was referencing the Spider-Man meme, since I recently built a hot glue web shooter. Technically I'm a musician...
@Joel Creates
Neato! I'll check it out.
-Also, I'm somewhat of a musician myself!-
Me too - my latest project is observing the time it takes to cook a '3 minute egg'.
I had a professor in college. This is the kind of project he was barking about in his often encouraged tangents during biopolymer class. Your project is like a customized and detailed exploration that goes well beyond what he could elaborate on in class. Thank you for peaking my interest!
Wow, what an incredible project! Your dedication and perseverance is an inspiration!
Thank you so much! Once I've got some fibers made, I'd be happy to send them over for you to play around with if you'd like
Here's an idea: Electron Microscopy videos of all the mineralised silks!
@@thethoughtemporium Maybe Ben could X-ray diffract image them, or run them through a mass-spec, or the Electron mic, or ALL THREE! amazing work, there should be a "UA-cam science medal" or something.
i think we are on the cusp of a revolution ... who needs elsevier , springer or iEEE these dudes together with a couple other sci-tubers are doing more cutting edge research than many well funded snob colleges.
@@thethoughtemporium Growing fibers is cool - But I would reccoment that you try mixing some into a Kombucha brew and make a SCOBY - you'll get nanocellulose tied to fibroin... it might be revolutionarily strong. Use weak/white tea if possible, and you can actually just use mineral supplements in sugar water for a "cleaner" SCOBY.
Also, that way, the yeast/bacteria make the sheet for you, and you don't have to worry about the fiber weaving process.
*shakes yeast vigorously*
"they're a bit of a diva ngl"
Someone give this guy a like a giant research fund. he deserves it.
IKR dis crap is insane
Where’s Oscorp when you need’em
Patreon
@@theunknowngamer5477 You know those movies are fake right lol
@@fuckdeadfaces for now...
"the plasmid has been shared on my github repo for that want to mess around with it"
imagine saying that sentence to someone in genetic engineering 25 years ago
well, we didn't have git back then
@@otheraccount5252 NO SHIT SHERLOCK
@@otheraccount5252 wow really
@@otheraccount5252 Really???? I thought github was like hundreds of years old omg thank you
@@Josuh the whacky part is that this sentence might someday be true.
it's insane that this is even possible. i feel like a caveman listening to this
advanced homeman
I am a software developer, but I am totally amazed how advanced and well understood biochemistry is.
I am a teenager, but I am totally amazed how advanced and well understood biochemistry is.
I am an idiot, but I am totally amazed how advanced and well understood biochemistry is.
@@themblue8236 lmaoo
I am a biomolecule, but I am totally amazed how advanced and well understood I am
@@mastershooter64 you win this thread
"It's [spiders] silk gene wasn't protected by any patents" you guys realize as well how nightmarish is this sentence?
Yup
Why?
@@carinhadoscomentarios4325 nobody should own the right to naturally-occurring sequences of chemicals
@@cooldud7071 big corpo preparing to sue every spider on earth
@@mikoajciemiega8018 Ohhh you little jumping spider you used my compound!? You are getting sued
As fellow You Tubers we continue to try to improve our content aware of the friendly "competition". You keep raising the bar...Damn
Excellent video, keep it up!
Thanks!!
hey Tech Ingredients I love your channel too
@@communist754 lol
@@thethoughtemporium You should collab with NileRed
@@communist754 why would he collab with someone who no one knows xD he doesnt even know if you know anything about the topic
Peter Parker : No one can figure out my super secret spider web recipe.
The Thought Emporium : Hold my beer.
isn't all beer made out of yeast though?
Hold my spider beer*
Hold my test tubes
@@bovanshi6564
Spiderbeer Spiderbeer...
Does everything a spider can...
Make a web? Anytime...
Look out...
Here comes the Spiderbeer...
Hold my beer but don't drink it. It's full of spider silk and tastes terrible.
Spiders: You will never be able to farm our silk...
Humans: Ok, we’ll just steal your genes and put them in other organisms.
Hippity hoppity your genes are my property
Surely nothing can go wrong when mankind tampers with nature...
GMO is scary stuff
@@nightelfmohawk9821 Well, I mean, spiders are scary too, I guess.
@@nightelfmohawk9821 And chihuahuas
@@nightelfmohawk9821 k but, how is farming spider silk dangerous
So what is the current status of this project? Have you scaled up the process and/or have enough of a sample to play around with for a video demonstration?
Would be incredibly interested in obtaining a sample of the yeast for a related personal project (involving kombucha SCOBY bacteria) if at all possible.
As a molecular biologist, the explanations in this video are better than most of my uni courses. Difficult principles made simple. Explaining something so that someone uninitiated can understand is a gift. Kudos for the job well done, your countless hours spent on this project were not in vain, sir!
My mind is blown. I knew bioengineering was a field that existed, but had never *really* thought about it. When I watch a video on chemistry or physics, I usually get most of it. I understand enough about chemistry to usually follow along with whatever video I'm watching, even if it goes to the slightly more technical side of things.
*This* video was 33 minutes of me going "wat." Okay, making yeast produce things. I've heard of that. Wait, you can just write DNA like you're a programmer? Wait, there are companies that will print DNA projects for you? This is common enough that a business can be made for it? Wait, you're taking a bunch of different genes and randomizing them and somehow you get the properties you want? Oh, silk sort of doubles back on itself for added strength; I get th- Wait, you can just add properties to it like it's a new spell in a video game? Wait...
Suffice it to say, I think the remainder of my day is going to be spent learning as much as I can about this. Fascinating is too weak a word.
that's exactly what was going on in my mind watching this video
Yep. I feel totally the same. I want to know more.
Same here... just... wow...
You've found your resolve my friend.
IKR it's like a game xD. You can just add whatever you want??? Lmao sign me up!
Pretty cool, and there's more power in presenting on UA-cam instead at academic conferences.
But my tenure!
A lot more. Get ready for an influx of scientists making UA-cam channels.
'With great power, comes great responsibility.
@@blackjoker2345 REALLY great profit margins
Man im so happy for you! I watched the first video a couple years ago and I was very anticipating the final and finished product! Congratulations on figuring it out and getting it to work!
"New merch coming soon! Who wants some homegrown T-shirt?!"
"I love it so much, I can't seem to take it off!"
The yellow ones are edible, but don't try the blue ones
Only $9,999.00. 😄🤑
@@GuilhermeCargnelutti I'm gonna eat the blue one
the colors wouldn't even need to be dyed. they could actually be from the silk it's self
I can't express how much I love that you document all this content for us to watch. Science in all fields can be so interesting, but it's so insular and stuck in the world of papers and journals that the average citizen will never hear about all the cool shit going on. You and many other science youtubers are really doing a great service.
It's often that insularity that convinces people to take up and believe pseudoscience and eventually erode trust in science and data
You call fax I mean we are all forced so deep into the thought that science is all boring stuff on paper but no it's fun aswell like artificial meat or aerogel and theories like our universe isn't finite and it's a loop from one point to another or that aerogel can be used in rockets as insulators.
I remember how in the action lab they mentioned that the structural strength of steel regarding tensile strength is in the gigapascals. Even specialized steel it's not like it's 10,000 times which spider silk can handle or whatever. Who knows how many creatures have a shell made out of nacre oh, it's tensile strength depending on the species is 170 gigapascals, even if one gigapascal is 2 megapascals this is still a whole lot more than steel. The trouble is this bad boy takes 170 gigapascals to bend, or is that bend it to a point that it will no longer Bend back. Either way that is a whole lot. I did the conversion for both of those, 170 gigapascals is more than 24 million pounds per square inch, and 70 is more than ten million. I did the conversion for both, I checked the conversion multiple times for the tensile strength. I'm pretty sure that I did the 70 kg pascals correctly given how much of it was copying and pasting, and using an online calculator. This is far beyond Steel. Now this is so strong because of the way that it is aligned, according to Google it's like more than 300 or something times stronger than the material it's made out of. So imagine graphene was in the structure that this material is in. And I'm not talking about the structure of which its atoms are aligned. This bad boy is made in a structure of pretty much interlocking tiles, so imagine this was what was going on with a flat thing of the functionally two-dimensional graphene oh, that bad boy would be incredible however graphene is very flexible so this might defeat the purpose. However something tells me that if the graphene were to be bio mineralized to be interwoven with the nacre be beneficial.
I can't imagine I live in the time when THIS is possible. You are in my "God-Tier UA-camrs" list
ShamelessDuck like you can print DNA?! that is kinda crazy, just humans writing their very own DNA designed specifically to hijack in this example yeast. I searched DNA printing on google and you can apparently make custom creatures with it, which is insane
@@tormodhag6824 I dream of future when we will use GMOs legally in our everyday life without it being marketed as "non-GMO" and without this whole stigma around it.
Ikr
And I love how the God-Tier UA-camrs all know each other XD
Applied-"I built a scanning electron microscope from scratch in my shop"-Science and Tech-"I made a nanostructure imprinted chocolate bar diffraction grating"-Ingredients both commented here alongside us! XD
Rewatching this video a second time and chuckled thinking about how people used to think everything was made of air, water, fire, and earth, and here you are doing this sorcery
Congratulations I know you've been working on this project for a while and it's really exciting to see you succeed
I’m just sitting here wondering how Peter Parker did all of this
I'm wondering why I've been searching for a Spiderman comment. Thank you
Same as tony, with a box of scraps but in a slightly nicer apartment.
did it in high school too ..,like how
The excuse is that Peter feels the necessity of making spider silk so that’s why he can make it? It’s really weird if I’m honest
Well Toby McGuire his webs were natural Andrew Garfields were stolen from oscorp and Tom Holland I don’t know but I’m pretty sure in the comics (I’ve never read them so this might be wrong) the spider bite gave him the formula composition and he was able to buy the products to make it
I'm an art student but I'm sitting here, 5:15 pm, watching a man make spider's silk out of yeast.
This is the coolest shit I've seen all day dude. Can't wait to see what you come up with next. Love the mad science bro.
23:42
"Thing number one.... be gentle with the yeast...."
I just love the way he said that
This was the fastest I have ever clicked on a notification.
Same
First- Oh fuck
Dito
Right lol
"I was able to purchase a whole vile of nightmare fuel for a grand total of 15 dollars"
I did not need to know the price of an arachnophob's sanity.
Secret Santa gone hardcore
@@minermortal1997 now THATS what I call an office party!
Someone needs to explain to me how any natural, unmodified living organism can have patents put on their genes in the first place...
Brian thats what we get in a a country run by corporations
Huh
I've been learning that sht to get marks from the 9th Grade and now I can use that knowledge to brag to a random guy on the internet because I know it and he doesn't
[I now realize that's all that information is every going to be useful for]
Lmao imagine existing and the big companies are like: "your genes are mine!"
Kinda reminds me of the tree that legally owns both itself and the land around it.
I know right, it seems that there would be some legal trouble with whoever made the patent and the original genetic designer of the animals, or would the actual spider be the patent holder of the silk since it originally created it, either way it doesn't exactly seem legal.
This has progressed my work farther and faster than I was hoping for. I appreciate your work and I thank you.
Glad to see this! 40 years ago I walked through a black widow web and began wondering about how to harvest the silk to make fabric.
Its so weird. He makes me feel like I can understand everything he is saying but at the same time I'm like
👁👄👁 okay 👍
4 mins in “what is he even talking about”
20 mins in “of course the pPCI9k needs to be linearized with a restriction enzyme! You can’t have those DNA rings, duh!!!”
it sounds like foreign language
I interpreted this as an informal video-essay type of report on his findings, truly fascinating, yet extremely confusing to people without bio-majors, like me ;)
"I've become quite competent at designing DNA from scratch"
"and just have a company PRINT the DNA"
Jesus christ watching your videos always makes me awe at the times we live in...
wait till he can print his own DNA without a company
Haha that spiders pretty hot amiright guys
Can't wait for the video on "spidey-sense".
@@BitterTast3 The real question: how would you go about achieving it?
Get bit by every spider
Spiderboiii
@@0th_Law Tarantula DNA? They use their hair to detect changes in the air. Spidey Sense was meant more to represent an invisible web of sorts, though I've also seen it as representing the 8 eyes spiders have though they actually have really bad eyesight. But by coincidence Tarantulas already have a spidey sense going for them.
It’s crazy going from “wow that’s pretty cool, no idea how you did that, but it’s cool” when watching science UA-cam to “huh, I did something similar in class last week, cool!”
Spidey yeast
Spidey yeast
Produces proteins a spider can
Makes a fiber any size, matches kevlar any time
Look out here comes the spider yeast
Is it strong? Listen bud,he's got enhanced gene strands.
Can it be made into a thread? Take a look over head.
Hey there goes the spider yeast!
Nice
Next project: yeast that makes spiders
Next next project: spiders that make beer
@@sleezymechanic next next next project:yeast that makes dimonds
@@notajalapeno4442 next next next next project: yeast that make any material
next project: watch youtube and feel like I have done something
next project: clean kitchen
*28:05** this moment left a big smile on my face*
*Congrats my guy*
I still can't believe people can write DNA in Google docs. I hope to do that someday.
Remember what he said tho
"I couldn't isolate the DNA, so I just made it myself."
...
I literally lost it when he said that
I will make my own DNA with hookers and blackjack
@@ProctasisLimerna and without the N.A.T.O!
@@ProctasisLimerna this sounds like a Cave Johnson quote.
I’m actually amazed at how much of your research I understood as a freshman biochem major. Stuff like this makes me hopeful that I could potentially make a career out of genetics because before college, I really doubted my ability to be successful in the field
never doubt yourself ;)
As a recent physiology grad this makes me hopeful to one day start working on unique avenues of research as well
Keep going. Who knows. You may pull a TASM again with the genetic spider 😂don’t give up on your dreams‼️
Well if you want to make something like Godzilla be realistic then try using biomineralization to put nacre in its bones and scales oh, you probably want pockets of stuff like methane in there, methane is a gas produced by living things the most common example that not many people might necessarily know has it in there is when you fart. But farting is made by the methane or swallowed air, by making it have nacre the bones would take literally millions of pounds to not only Pull-A-Part but also Bend, or is that bend it to a point that it will no longer be able to bend back to its original shape. The tensile strength is 170 gigapascal, there are 70 for its strength for the bending thing. 170 gigapascals after I checked my conversion this correctly comes out to more than 24 million pounds per square inch, even if you use the metric system that is obviously a very high amount. The action lab which is a another Dependable source of science information said that steel is generally in the megapascal range, it takes a lot of megapascals to make a single gigapascal. And if its skin is made out of graphene, or whatever term is used for the soft flexible stuff that is what is responsible for holding the scales together on the skin of a snake or lizard. That bad boy would be very durable, for context just some graphene that is the size of Saran Wrap would require Force equivalent to a fully grown male African elephant balancing on a pencil. Not to mention that the Wonder material is also a very effective superconductor so you could probably use this to get rid of the issue of the Godzilla that is more than 100 m tall taking more than a second for anyting to register in its brain because the electricity take so long to move through the body. This graphene could eliminate that issue oh, so we could react without needing to be 15 M tall at maximum. And if it's hunched over like your average T-rex or Utahraptor this could help with blood flow at such a gargantuan scale. And if you argue that it doesn't have enough energy from the atmosphere to oxygenate it, there is an organism that can survive without any oxygen, also considering out at our degraded can literally live for multiple days in the vacuum of space. Something tells me there's probably the required already existing genetics somewhere in the world to let something survive at such a gargantuan scale. But maybe not after all a hundred meters is a very high number for the size of an organism on Earth.
Nice👍 I plan on majoring in biochem
The dislikes are just the other spiders being jealous about this amazing silk.
And the big corps geting mad because the plasmid is open source
I can't believe you were able to explain this in a way I almost completely understand! I learned SO MUCH from this!
I would be interested in where LOTUS silk falls on the graph at 10:10
This is huge, it's amazing how you managed to succeed, this sounds like science fiction and yet you made it reality, I admire you
Everyone here who has no clue what you’re saying just going along like “ah yes the graphene binding peptides have worked”
It's actually decently intuitive. If the yeast produces a protein with the ability to bind graphene, then the graphene oxide will naturally coagulate around the proteins, and therefore the yeast, because it is being bound to the proteins.
@@0th_Law hUh 😐
@@TheIdiotFr0102 If you want, I can clarify stuff for you; just tell me what doesn't make sense. (This is assuming that you mean that what I wrote didn't make sense. If that isn't the case, then never mind.)
@@0th_Law you said some smart stuff but I think this comment is meant to be a joke and you added some smart stuff to it, so idk
@@arrowsaurus7561 You're going to have to be more specific than that. Otherwise, the answer will probably just be something like "Cell Signaling" or "Conformational Changes"
Giving a whole new meaning to “silky smooth beer”
Been following your progress on this for so long. It's awesome to hear you're over that major hurdle!
I look forward to hearing how the rest of your experiments go!
We have been waiting for this for sooo long!!!
Spider-Man’s web fluid is now becoming a reality
FAVIAX Delatorre hol up
Indeed it is, my friend
@FAVIAX Delatorre 😤😤😤ಠ‿ಠ
@FAVIAX Delatorre That's f**ked up.
FAVIAX Delatorre get out.
All i can imagine is him looking at the genetic code and going "mmmm, silky"
so smooth
There's something silky going on......
The FACT this all evolved through eons of probability is astounding
Make the web cure faster is hard enough. Let alone:
1. It can carry the man size weight
2. Produce a length of string
3. Infinite strings.
4. Become spiderman
@@sonofanutcracker4207 5. Great responsibility
@@darknobz3560 great stress and late rent debt
@@urtooslow4415 7. *MENACE!!!!*
@@benbruder 8. Get forgotten by everyone
Just leaving this out here: A silk/graphene composite generating yeast that survives high salinity would be prime candidate for regenerating tidal turbines
I understood all of those words separately and am proud of myself for that.
If this is true, on top of making it a commercial clothing or building material, this guy could be looking at a Nobel.
I think that could do a lot more than just repairing turbines
While it could do that, would it not also coat the ocean floor in carbon doped silk?
@@thecallankids4718that I’d assume degrades faster than thousands of years
"A whole vial of nightmare fuel" I've never heard that be so damn accurate
I’m pretty sure that’s a statement you’ve never heard before, or in the two years since. So that’s not particularly impressive
@@BahhBahhBrownSheepCounterpoint: the Pony Jar Project exists.
make a web shooter and only if the silk was strong enough, you could be spiderman.
All the people who want to be Spiderman: *_confused but encouraged_*
"Write that down! Write that down!"
Lmaooo why would you call me out like this!!
Lol I was in 7th grade and the 2002 Spider-Man came out. My friend and I thought we could make web shooters from aluminum cans and webbing from kitchen spices and cleaners.
Aight, that's me right now
I want to make a web shooter
Ive been tryna figuring out how to be spider man for the past 3 yrs lol, and I DIDN’T RUN A SINGLE TEST BECAUSE 12 YR OLDS CAN’T GET A HOLD OF NYLON 6, 10 SILICONE AND ADHESIVE GEL. I ALSO WANTED TO ADD HIS SPIDER SILK LIQUID THING BUT I’M TO “UN-INTELLIGENT” TO MAKE IT. AND DON’T ASK WHY I’M YELLING BECAUSE IDK
Type of guy to clone himself and go on vacation and we won't notice
KRIEGER!
This guy: *invents yeast spider silk*
Everybody: SPIDERMAN SPIDERMAN DOES WHATEVER A SPIDER CAN
it does add another meaning to "spider can", right?
Yeah, i was especting that. But i'm more interested in the practical use of spider silk, cause it's resistance is legendary.
@@kiriki4558
I was referring to a Beer-CAN full of silk. (assuming you are replying to my comment that is)
The practical applications are a no brainer.
10 years ago i would have shelved this under sci-fi, jet here you are actually doing it.
btw, the biomineralisation feature sounds amazing. I cant even imagine what properties spider silk bonded to carbon nanotubes would have, but i'm sure it would get you out of a sticky situation, or create them for that matter.
This is by far the most incredible, most inspiring thing I've ever seen. People like you are the future, I truly believe that. Unbelievable.
“Was able to purchase a whole vile of nightmare fuel”
Lol, he's not wrong.
23:07 Stuff like this is why I love the internet. It's so nice to see researchers come together and help each other out :)
Everyone watching this that has no idea what he’s saying: “You know, I’m something of a scientist myself.”
¿Is that that a Raimi reference?
@@estebancino5426 no.
@@cleosvoyage yes - the quote is from sam raimi's spiderman movies
I studied abt genetic engineering
@@ytshorts9609 I studied the entire ABC of English alphabet and got it all memorised now! Pretty cool, huh? 😎
Vortexing nad pipetting up and down is one _hell_ of a difference of force.
I have 0 biology and chemistry skills, but I love to see similar concepts and ingenuity you can find in many engineering fields! This is amazing!
I literally wrote about you in my university application. You're the reason I'm going to university. Thank you for creating videos and making them high quality and interesting!
I think you did a really good job on the visual side of this video, the diagrams, infographics, tables etc. As a graphic designer I was impressed. Awesome video.
dude you are insane, an insane genius, instead of modifying an organism, making a new spider, we get spider beer, spider beer concrete, spider beer kevlar, spider beer plastics. man, respect
Biology brain me: ah yes an excellent video, a true breakthrough, very exciting.
Normal brain me: hehe pretty colours
I honestly didn't understand what you were talking about. But I was intrigued the entire time. I didn't even know it was possible to PRINT DNA. This is some crazy stuff
Why am I watching this, my experience with biology is “the mitochondria is the powerhouse of the cell” and “sometimes, plants eat the sun.”
I’m imagining a plant just biting a giant chunk out of the sun
@@joshuanetanel9174 Of course, the kale kills.
Soylent green is people too.
lol, same, I'm just curious to see what emerges in the next 25 years from him & this field
i would love to see a plant eating the sun
11:00 thanks to high school biology, I can actually understand this. Thanks for showing a interesting use for the stuff we are learning!
This is so cool, as a researcher who works with genetically engineering microorganisms to produce proteins, this is a huge inspiration
"First: It's silk gene wasn't protected by any patents" whoa whoa whoa, hold on, what? What kind of cyberpunk is this?
I'm cool with that. Assuming the spiders are the patent holders.
But that strikes me as being somewhat unlikely.
@@herrpez spider lawyer
@@SomethingSmellsMichy HAHAHAHAHHAHAH
Spider Lawyer, Spider Lawyer, does whatever a lawyer does, does he defend? Yes he does, he can also climb the laws, watch out here comes Spider Lawyer!
Man really said "fine. I'll do it myself."
I’m 15 and I don’t understand most of this but this has inspired me to learn more about biology and also I’ve designed my own web shooters if I could find a way to get the silk liquid.
Everyone’s talking about him using big words. Me thinking how he could be the next Spider-Man
"Could be used for -coughs and shows picture of spiderman- s p e c i f i c uses"
I'm thinking about spider kevlar
They might not all SAY it, but we were all at least thinking it.
I think in the Korean war they used spider silk for an bullet proof vest. But nowadays the bullets have more energy so it doesn't protect anymore
@cosmify Check the video at 20:25 hahahaha
"First, its silk gene wasn't protected by any patents…"
Wait, WUT?
The first entity to isolate a particular gene gets to file a patent on it under the current biotech regs. Leads to some very undesirable possibilities. It's one reason to be suspicious of those 'we'll analyze your DNA to determine your ancestry/risks' things, because it's potentially a mechanism for someone to gain a monopoly on all commercial use of one of your genes for the next few decades.
capitalism
@@wacesferpit That's the more succinct explanation yeah.
@@Plotatothewondercat ... and then (after the 20 years) your own personal gene is open to the public to use as they see fit.
@@eichenbrain6170 Twenty years after a corporation had the opportunity to raise the price of whatever treatment it may have turned out to be useful for to the point that thousands of potential beneficiaries went past the point of no return without ever having the capability to pay for the treatment.
Oml I am learning this in my AP Bio class as we speak: gene expression. Seeing it used in a real world scenario and having you explain this made my life so much easier! Thank you, and continue doing cool stuff.
“I grew real spider silk using yeast!”
*Peter Parker wants to know your location*
I'd recommend learning the basics of python or another simple scripting language. Your method of random generation of genes is very cool, but a script could be doing all that copying/pasting for you and churn out genes as fast as you can ask for them. You can also have the script do Longest Common Sub-sequence analysis to ensure there's no large repeating blocks that could mess up printing (I assume the synthesis companies do something like this on their end when deciding which orders to reject).
Also, your videos have taught me more about practical genetic engineering than years of school ever did. Good shit 👍
also, why? whats wrong with the sequencer? are you telling me its inappropriately named?
I've thought about that too lol. I usually write a quick script for stuff like that.
This guy is impressive as hell. The dedication, trial and error, the learning process along the way, and never giving up...Amazing and inspiring! 🕷🕸
Peter Parker: Would Like to Know Your Location
Underrated comment😂
@@ashketchum6585 Indeed. It could have 10x the likes, and still be underrated.
Mad Scientists in movies: The world will be mine!
Mad Scientists IRL: spider beer, spider beer, does whatever a spider beer does...
I met you at the end of last winter and I remember you mentioning that you had done a lot of progress on spider silk production. Glad to see it all worked out. The video's really informative, too. I'll try to visit Foulab again after this whole pandemic ends.
I hope to get a chance to meet him someday as well.
This is literally the forefront of today's science. I don't believe anyone has come this close.
Most researchers: OK, so we grew the spider silk, now how do we add additional elements to it?...
Justin: screw it, let's add a gene that binds Calcium, Silver, and Graphene for good measure.
This is actually really interesting. I also sometimes forget that people just do this kinda stuff for fun/a living.
Me after watching this video: *"You know, I'm something of a scientist myself."*