Calculations for Cell Culture Part 3: Subculturing

A walk-through of the calculations required for subculturing cells. How to work with manufacturer instructions for required cell concentrations and how to get different volumes.
Part 1 can be viewed here: • Calculations for Cell ...
A simple overview of the calculations required for cell culture, including calculating cell number, dilution factors, and how to make suspensions at certain concentrations.
Part 2 can be viewed here: • Calculations for Cell ...
A simple overview of the calculations required for the trypan blue assay used for cell culture. This is used in subculturing and also to work out viability.

[@_M3gh]

Thank you!

[@SaffronBryant]

You're welcome! I hope you found it helpful.

[@freespirit6855]

Your cell counter says 5x10^5 cells/ml. .you had a stock of 12 ml and you want to replate in a T75at 0.1x10^6/ml. Find the dilution