Immunohistochemistry Explained: Principle and Techniques for beginners

This video lecture explains
1. What is the definition of immunohistochemistry (IHC)?
2. What is the principle of immunohistochemistry?
3. What are the different steps involved in the immunohistochemistry procedure?
4. How sample is prepared in immunohistochemical analysis?
5. What is antigen retrieval and why is it important ?
6. How and why blocking is performed in immunohistochemistry?
7. How target antigen is detected?
8. What are the differences between fluorescence-based detection method and colorimetric based detection method ?
9. How the samples are visualized in immunohistochemistry?
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[@sendachiyesu8268]

What i failed to understand in 2 days i understood in 11minutes thank you. 🙇

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[@Hari788]

Awesome explanation. Please make more videos on cell biology. Thank you very much for this.

[@BiologyLectures]

Sure we will. thanks

[@Amber-gc5cv]

Thank you, this was very helpful!

[@BiologyLectures]

You are most welcome.

[@andriaterrill527]

Excellent video. Super clear and super helpful! Subsribed!

[@BiologyLectures]

Thank you very much 😊

[@hunters7638]

the definition is very clear ! super clear

[@BiologyLectures]

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[@NK-DF-ST-10-3]

Thanks the training was amzing i learnt a lot

[@BiologyLectures]

You are most welcome.

[@craigcorner6170]

Hi. Just one thing, the colorimetric method if IHC depends upon an enzyme and not a substrate being conjugated to the primary or secondary antibody. The substrate (DAB or FAST RED for example) being added in subsequent steps to produce localised (brown or red) coloured precipitate for visualisation
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Yes, we totally agree. Thanks for your valuable comment.

[@vedadhruthy8310]

Explained very clearly and precisely 👌

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[@SDscienceproductions]

Very well explained thank you

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[@hassanhassan7373]

THANKS
GREAT INFORMATION

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[@JR-gv4ew]

Thank u so much Sir.. So clear

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[@kamalapokhrel7700]

THANK YOU SO MUCH

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You are welcome

[@dikshyapanthi7681]

Best explanation 🙏❤️

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Glad you think so!

[@danielkamwagha3824]

I love your explanation

[@BiologyLectures]

Thank you

[@nilanjanmukherjee8043]

Very helpful ❤️✨

[@BiologyLectures]

Glad it was helpful!

[@ogunlayiaderonke785]

Thanks

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You are welcome

[@shormilyrajui3627]

I ❤ your explain

[@BiologyLectures]

Thanks a lot ❤️

[@mayeenuddin6135]

Dr done biopsy didn't told me the result and tell me for IHC test . Does this mean my sample has malignancy?

[@shormilyrajui3627]

❤

[@BiologyLectures]

Thanks a lot and all the best.

[@Bhairav1256]

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[@BiologyLectures]

No.

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