Hi, I checked the antityrosinase activity of my sample at 200, 400, 600, 800 at 1000 μg/ml and the results were 1.25, 3.925, 5.775, 6.625 and 8.75 respectively. I used kojic acid as standard at the same concentrations and got 16.437, 24.625, 32.625, 47.5625 and 86.75. I used Y=mX+C to calculate the IC50 values and I got that of my sample to be 0.2667μg/ml and kojic acid to be 702.55μg/ml. Please I need someone to use graphpad prism to validate my result 🙏
Then it's very probably that you don't have the same concentrations values for your samples, in that case you can calculate the ic50 separately for each sample
@@AccroInformatique if I get the conc which give half absorbance This give the dose which kill half population And sometimes with inhibition rate some doses give negative result which can't put it in the table
Please, who Can Help me. I use a molecule that stimulates the cell growth at a low dose but it inhibits the growth at high concentrations. I want to determine the IC 50, the concentration that inhibits the growth of 50% but the graph is biphasic. How can I determine the IC 50?
Hello, thank you very much for the video. A question: can this procedure be used to determine the dose of fungicide that inhibits 50% of mycelial growth of a fungus in a petri dish?
Hi many thanks for the useful video May I ask plz why you are adding a 0 value in x points vs 0 values in y axis corresponding to the zero in x axis? Thanks a lot
Hi thanks for your comment The point (0,0) is a known point because in my case there is no absorbance without concentration, this will help you with the regression
@@AccroInformatique Ok and thanks brother for your reply. But I send a images personally... If you don't mine can you send your email id. After that I will send you.
@@AccroInformatique it's my humble request. Please start with gel picture, how to get the values. After that how to do it two way annova prism. I can't share my pictures public. That's why I requesting you.
9:38 When I was typing the values 50 at the end of each groupe there was an error in the last group and I typed 505 instead of 50, I just corrected it. Thanks for your comment
@@AccroInformatique For example, I need to calculate SI ( IC50 on non-transformed cells (MRC-5)/IC50 on tumor cells). If investigated agent doesn't inhibits growth on MRC-5 over 50%, I still need assumed IC50 value for MRC-5 to calculate SI.
this video was very detailed. is it possible for me to calculate the IC50 value using absorbance instead of percentage inhibition? Also what if I tested my sample at different concentration and none of the concentrations inhibit 50% does that mean I wont be able to calculate the IC50 or it just means I will have an extremely high IC50 value?
hello thanks for your comment, the IC50 is a concentration not a percentage, IC50 means the concentration value that inhibit 50% of the population so you can't do it without concentrations values. if you tested your samples at different concentrations and none of them inhibit 50% that mean you must prepare high concentrations to get the IC50, if you do that and you still having the same result that mean your sample have no effect
Trust me best vedio on using Prism for IC50 calculations, highly appreciate your efforts.
Thank you so much for your kind words bro 🙂🙂
your video is so perfect! I don´t have
words. I love it
What is the difference to calculate IC50 between this method and log(inhibitor) vs normalised response-variable slope.
any info about
Thank you for the very helpful video
. I repeated exactly every steps but the interpolated x values column appears empty !
Thank you for the video. Meanwhile, at 10:32, how did you make the 8 values under concentration 9? Thanks
The best explanation!!
Thanks Amanda 😀👌I really appreciate that
Thanks a lot, its a huge help.
You're welcome happy to hear that, thanks for your feedback 🙂🙂
How did you get the 3rd value (23.896) of the interpolated X? It was not displayed initially. Thanks
I think I figure it out, one of the 50 value I added was missing when I added it the value appeared.
Thanks for the video. Does it only work when values are greater than 50?
really thanks for the very helpful video
i repeated exactly every step but the interpolated x values column is empty!!!
Man you saved my life. ❤❤❤❤
So happy for you 😀, thank you so much for your support
@@AccroInformatique plz keep uploading informative videos. Grateful for this one.
@@Thesisandbeyond you're welcome bro, i will do my best inshallah 😀
While calculating the p value, I am not getting the values for 1 on 1 collumn. the p value is kind of collective of all the coloumns. What to do
I'll thank you to do one tutorial for bacterial growth. All my Thanks again
that was onpoint! thank you
You're very most welcome 😀, thanks for your time 😎. Please don't forget to subscribe and share the video with your friends ✔✔✔
very interesting video. thank you so much
Thanks for your comment 😀
Thank you very much is very useful
You welcome
So helpful!!!!!!! Thank youuuu soooo muchhh
You're welcome 😀
Very intresting. Thank you
If you can do the similar video for H2O2 inhibition
God bless you and thank you so much
You are welcome 👍
Hi,
I checked the antityrosinase activity of my sample at 200, 400, 600, 800 at 1000 μg/ml and the results were 1.25, 3.925, 5.775, 6.625 and 8.75 respectively.
I used kojic acid as standard at the same concentrations and got 16.437, 24.625, 32.625, 47.5625 and 86.75.
I used Y=mX+C to calculate the IC50 values and I got that of my sample to be 0.2667μg/ml and kojic acid to be 702.55μg/ml.
Please I need someone to use graphpad prism to validate my result 🙏
I follow all your instruction but after analysis the X interploted values didn't appear could you help me in that ?
Then it's very probably that you don't have the same concentrations values for your samples, in that case you can calculate the ic50 separately for each sample
THANK YOU SO MUCH
You're welcome, please don't forget to subscribe and share the video with your friends, thank you for your comment 😀👌
Donee
Thanks for your support 😄
Can I put absorbance verses concentration instead of inhibition rate ???please answer me
And put .5 instead of 50 value
Is it correct method ,???
IC50 is the concentration that inhibits 50% of the population, so you can't calculate it without the inhibition rate
@@AccroInformatique is the role of inhibition rate = 1-(abs treated /abs control)×100
@@AccroInformatique if I get the conc which give half absorbance
This give the dose which kill half population
And sometimes with inhibition rate some doses give negative result which can't put it in the table
Please, who Can Help me.
I use a molecule that stimulates the cell growth at a low dose but it inhibits the growth at high concentrations. I want to determine the IC 50, the concentration that inhibits the growth of 50% but the graph is biphasic. How can I determine the IC 50?
Hello, thank you very much for the video. A question: can this procedure be used to determine the dose of fungicide that inhibits 50% of mycelial growth of a fungus in a petri dish?
Hi many thanks for the useful video
May I ask plz why you are adding a 0 value in x points vs 0 values in y axis corresponding to the zero in x axis?
Thanks a lot
Hi thanks for your comment
The point (0,0) is a known point because in my case there is no absorbance without concentration, this will help you with the regression
Sound kyo nhi h video me
its very informative video, I have one question why you calculate one way ANNOVA?
??
Thanks for sharing!!!
My pleasure!! 😃👌
Thank you very much
You're welcome, hope you subscribe to the channel and share the video with your friends 😀👌
The role to calculate inhibition rate = 1-( abs treated / abs control) x 100
Is it true??
Mercie Mercie mercie❤❤
Merci pour votre commentaire 🙂
Very helpful
Thanks 😀
Can I use the days instead the dosage?and obtain the lethal time 50?
Nice video
how to make a graph on gene expression studies ? could you explain the video.
Thanks brother, of course you can just send me a data example, so I can know exactly what you want to do
@@AccroInformatique Ok and thanks brother for your reply. But I send a images personally... If you don't mine can you send your email id. After that I will send you.
@@AccroInformatique it's my humble request.
Please start with gel picture, how to get the values. After that how to do it two way annova prism.
I can't share my pictures public. That's why I requesting you.
@@kandasamysaravanan9832 yes brother i really understand, this is my email accro.informatique@gmail.com, i will do my best in chaa ALLAH
@@AccroInformatique thank you I will send you soon
Hello sir could you explain how you added the missing interpolated value.
9:38 When I was typing the values 50 at the end of each groupe there was an error in the last group and I typed 505 instead of 50, I just corrected it. Thanks for your comment
@@AccroInformatique I entered my data correctly and missing values are still not coming up
Thanks alot❤
You're welcome 🙂
my graph line show opposite lift side
Can you explain how to calculate IC50 when effect on the highest concentration is below 50%?
Thank you in advance!
Hello @Sanja Matić
, can you explain your question better, because we can't say IC50 unless you have a concentration that inhibit 50%
@@AccroInformatique For example, I need to calculate SI ( IC50 on non-transformed cells (MRC-5)/IC50 on tumor cells). If investigated agent doesn't inhibits growth on MRC-5 over 50%, I still need assumed IC50 value for MRC-5 to calculate SI.
@@sanjamatic8767 You need test MRC-5 in those doses that can kill from 0% to 100%. Now when you perform the calculation you can get the IC50.
Haii can somebody help me how to calculate ic50 of two drugs that had no constant ratio???
can send the data table again?
this video was very detailed. is it possible for me to calculate the IC50 value using absorbance instead of percentage inhibition? Also what if I tested my sample at different concentration and none of the concentrations inhibit 50% does that mean I wont be able to calculate the IC50 or it just means I will have an extremely high IC50 value?
hello thanks for your comment, the IC50 is a concentration not a percentage, IC50 means the concentration value that inhibit 50% of the population so you can't do it without concentrations values.
if you tested your samples at different concentrations and none of them inhibit 50% that mean you must prepare high concentrations to get the IC50, if you do that and you still having the same result that mean your sample have no effect
❤❤
how to make bar graph of dpph samples .after ic50
Can we calculate NOEC values using prism 8. if you know please share. thanks
if i were to calculate LC90, do I just need to change 50 to 90?
Yes you're absolutely right 👌👏👍
@@AccroInformatique Thank you so much for the video
God bless you. .... awesome////
You're very much welcome
Where is standard
Thanks
you are almost welcome, please don't forget to subscribe and share the video with your friends, thanks for your comment
Aprendi de um jeito totalmente diferente, além disso, esta forma não da o R², nem o intervalo de confiança de 95%. Estranho.
Thank you for the very helpful video
. I repeated exactly every steps but the interpolated x values column appears empty !