Retrieving Sequence - Complement and Reverse Complement - Bioinformatics Practical 1

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#Retrieving_sequence_in_correct_orientation - In this lecture, I describe the basic concept of Sequence - #Plus #strand, #minus #strand, #complement and #reverse #complement and also explain the steps to #retrieve the sequence of the gene from #UCSC #genome #Browser
#sequence #plus_strand #minus_strand #complement #reverse_complement #UCSC_Genome_Browser #lets_grow_together #vipins_e_classroom #bioinformatics #biotech

[@Sing-with-Amna]

Respected sir
I am a biotechnology student and being a biotechnologist i personally feel that a biotechnologist must be expert in bioinformatics tools.
I have knowledge of wet lab but not of dry lab
I have taking the lectures from your lectures series and you have taught in very good way.
Thanks you and keep it up

[@vinicius_stelet]

Thanks for sharing, professor.

[@classroom_vipin]

My pleasure! Do share with your peer group too.

[@user-uo4kn1oh8y]

Very nicely explained Sir. If we do not mask the repeats then also it will be shown in the DNA sequence ?

[@classroom_vipin]

Yess! Then it will be shown as a regular sequence I.e. you won’t be able to discriminate between what’s a repeat sequence and what’s not !

[@ranakamei]

Thank you, Sir, for your clear explanation of the reverse complementation. But I am confused. How do we determine a DNA strand as either + or - (plus or negative) strand? What is the reference to it?

[@classroom_vipin]

For this you have to understand how a DNA is generally sequenced - Most Sequencing technologies use DNA polymerase and Forward and reverse primers to sequence the fragment from both ends - the sequence coming from the forward primer is considered plus - check out my Sanger sequencing video in the channel also NGS play list

[@babadhriti]

why there are lower case letters?

[@classroom_vipin]

You may have selected for repeat masking to lower case option in the get dna window - UCSC browser