I don't understand in every lecture many of them will say excellent, super, very good. I have a few questions on Gene silencing. (i) What is the role of retroviruses and transposons and heterochromatin in gene silencing. (ii) How nematode specific gene is cloned in tobacco plant. (iii) Would nematode parasite release its m-RNA into host cytoplasm such that host plant with cloned gene produce antisense RNA that base pair with Nematode m - RNA, Explain the process. (iv) How both sense and antisense RNA are produced in tobacco plant cloned with nematode specific gene. (v) How cDNA produce antisense RNA.
Sir thank you very much, I have an exam day after tomorrow it's very informative but I want to know about what the role of this technology in target discovery and target validation and I request you to make a video on this plssss.
Sir I am having a doubt in one of the part, where u said RNase H is used to break the mRNA but RNase H is only degrading the RNA which is in hybrid form i.e. with RNA: DNA hybrid.
very good explanation but I have a question, as y said that anti scence RNA is complementary to targeted mRNA , then how can this blocked the translation instead of regulating. Is there any special difference between complementary Antiscence and mutated one.
According to this simple technique If the complementary RNA attachs on targeted RNA it will take help from RNAse H enzyme and it will break down the Targeted mRNA and we 'll get rid of all mrnase nd there will be no chance of production of protein
Thank you Shomu. One Question: since the original bad DNA sequence remains in place in the body, it will continue it's propensity to create the "bad" mRNA, so one has to continue to take the anti-sense therapy FOR LIFE?
While I'm not the gentleman, I know some about this. You are correct. However, there are upsides to short duration therapies. If a mistake is made, or if the side effects are greater than the symptoms of the disease, you can quickly halt the therapy and revert to former physiology. RNA therapy is also very tunable; assuming effective, consistent transport into the cell you will have a predictable, concentration/does based effect.
@@jeffbrannon1757 it's all very interesting stuff. Some time bakc i was think of the monoclonal antibody market! it's huge. RNA vax technology can 100% disrupt that market! rather than taking antibodies therapeutically, every day, one can get an RNA "vax" to use your body to generate the antibodies! however, covid has shown that even that is not a long term therapy, you need to take it again every 4 months.
Antisense creates a single strand of DNA. Not RNA, that is how the RNase H works. As it recgonizes the hybrid of DNA & RNA. It does break down the RNA, but it is a single strand of DNA, not RNA.
I created a new treatment concept for DMD/Beckers, I am presenting it at the NORD Rare Disease Conference in Washington D.C. next week. AON technology is a part of my treatment concept.
Wow...!I can't say what I feel after watching this video...Just amazing...
Thank you
Mind blowing sir , this topic is completely set in my mind
Glad to hear that you're getting benefit from my lectures
Ur vdeo helped me a lot today in my project
You're welcome
This video was truly helpful Sir.
You're welcome. Glad to hear that you're getting benefit from my lectures
Super teaching sir....
You're welcome. Glad to hear that you're getting benefit from my lectures
My father is suffering from ALS😞pls pray for him🙏
Don't worry man he will be fine soon 😊
Sir,Your videos helped me to complete graduation......love and respect from Bangladesh
Thabk you. Glad you liked it and
Excellent explanation with clarity👏
Thank you
Thanks a lot for the lesson. Superb indeed.
You're welcome
excellent and simplest way of demonstrating. goooooood
I don't understand in every lecture many of them will say excellent, super, very good.
I have a few questions on Gene silencing.
(i) What is the role of retroviruses and transposons and heterochromatin in gene silencing.
(ii) How nematode specific gene is cloned in tobacco plant.
(iii) Would nematode parasite release its m-RNA into host cytoplasm such that host plant with cloned gene produce antisense RNA that base pair with Nematode m - RNA, Explain the process.
(iv) How both sense and antisense RNA are produced in tobacco plant cloned with nematode specific gene.
(v) How cDNA produce antisense RNA.
Helpful.... thank you sir 😊
You're welcome
Thank you Sir 🙏❤️
You're welcome
thanks for this video siir
You're welcome
Very useful...thnku sir..
Thank you so much sir
You're welcome
Sir thank you very much, I have an exam day after tomorrow it's very informative but I want to know about what the role of this technology in target discovery and target validation and I request you to make a video on this plssss.
Khoob bhalo sir
Dhonyobaad
@@shomusbiologyofficial sir aapnar video dekhe 11th,12th,Bsc paash korlam or ekhun MSC te oo podchi
excellent sir....
Hi, can Ataxia Telangiectasia be treated with antisense oligonucleotide gene therapy ?
Thanks a lot brother.
Thank you
very good lecture ! good work !
thanks Lot !
Great video. Thanks
Why does rnase h does not degrade the antisense RNA and only the targetted RNA?
Sir your videos are just perfect but there's slight problem with the sound. It's really very low
Sound is fixed later
Sir I am having a doubt in one of the part, where u said RNase H is used to break the mRNA but RNase H is only degrading the RNA which is in hybrid form i.e. with RNA: DNA hybrid.
Sir, dna sequencing antisense ??
great video sir
sir plz make a video on C4 cycle
sir plz make video on different models of dissolution kinetics
Thank youu.
thanks for this :)
wonderful video , very interesting thank you.................
Tq sir
You're welcome
sir will you plzzzzz upload the vedio on Ribozymes. its my request sir plzzzzz
Sir pls suggest any book for this topic
very good explanation but I have a question, as y said that anti scence RNA is complementary to targeted mRNA , then how can this blocked the translation instead of regulating. Is there any special difference between complementary Antiscence and mutated one.
According to this simple technique If the complementary RNA attachs on targeted RNA it will take help from RNAse H enzyme and it will break down the Targeted mRNA and we 'll get rid of all mrnase nd there will be no chance of production of protein
Thank you Shomu. One Question: since the original bad DNA sequence remains in place in the body, it will continue it's propensity to create the "bad" mRNA, so one has to continue to take the anti-sense therapy FOR LIFE?
While I'm not the gentleman, I know some about this. You are correct. However, there are upsides to short duration therapies. If a mistake is made, or if the side effects are greater than the symptoms of the disease, you can quickly halt the therapy and revert to former physiology. RNA therapy is also very tunable; assuming effective, consistent transport into the cell you will have a predictable, concentration/does based effect.
@@jeffbrannon1757 it's all very interesting stuff. Some time bakc i was think of the monoclonal antibody market! it's huge. RNA vax technology can 100% disrupt that market! rather than taking antibodies therapeutically, every day, one can get an RNA "vax" to use your body to generate the antibodies! however, covid has shown that even that is not a long term therapy, you need to take it again every 4 months.
I want post transcription modifications detailed plz
Will post it soon.
🙌
Antisense creates a single strand of DNA. Not RNA, that is how the RNase H works. As it recgonizes the hybrid of DNA & RNA. It does break down the RNA, but it is a single strand of DNA, not RNA.
I created a new treatment concept for DMD/Beckers, I am presenting it at the NORD Rare Disease Conference in Washington D.C. next week. AON technology is a part of my treatment concept.
I do love your videos, and have learned a lot from you. So thank you.
Yes u r absolutely right@August Gene.
Morpholinos
who is here in 2022?