Silver staining gels - the biochemistry of how it works

[@mhchang1555]

Thanks for provide a video full of useful detail information and make it very easy to understand.

[@MsManojit]

Thanks so much for the detailed explanation - it is great to understand the process behind every step of the experiment instead of blindly following a protocol from a kit!

[@thebumblingbiochemist]

Thanks Manny! So glad you found it helpful!

[@Mycosis_]

Thanks for the video, so after you stain the gel, can you destain the gel to restain with silver staining?

[@thebumblingbiochemist]

Yes, you can destain silver stains

[@wendelun]

Thanks for the great explanation. I did a silver stain once on a 12% bis-tris gel, and then stored the gel in water. The gel swelled up to twice the size of the original gel. This never happens with a colloidal blue stained gel. Do you know why it swelled up after silver stain? Thanks.

[@thebumblingbiochemist]

Maybe there's a higher concentration of silver inside the gel than concentration with coomassie?

[@biswajitbhowmick2689]

Nice