Selection and Characterization of hSTING-Agonists via in silico modeling and SPR

In this study, Cayman Chemical performed our established computer-aided screening techniques using Schrodinger to select hSTING agonists for further characterization via Surface Plasmon Resonance (SPR). We established a SPR platform to evaluate binding kinetics and affinity measurements for the agonists. Synthesized agonists such as canonical CDN (2’,3’ cGAMP), amidobenzimidazoles (diABZI agonist-3), and purified recombinant hSTING variants were used for SPR analysis using a Biacore instrument. Here we report the selected agonists affinities was improved compared to previously reported values. These findings could provide guidelines for the design and development of hSTING agonists research.
To cite this poster: Assar, Z. and Hines, J. Selection and Characterization of hSTING-Agonists via in silico modeling and SPR A. Poster presented at: Keystone eSymposia on Innate Immunity: Mechanisms and Modulation; April 12-15, 2021.