Sir I'm from telangana urs Tamil is easy to understand and my friend recommended me. Really it's very helpful to understand and special thanks to u both. ✨
I was really scared about this cloning vector because I thought it was complicated so I didn't touch it for a long time but now it looks very simple thank u
Bro semma bro, what u r major bro........................ Neet and great teaching bro, seriously bro yea padikuro ethukaga namala itha padikuro ithu enga use aagum ithulla ethukaga varuthu yea ithulla sollurainganea theriyamma ethos padikka irukku so padikanumea nu irukku intha educational system ......................................bro sathiyama sollurea ipo tha intha cloningea super ra understand aairukku , 10000 of thanks bro Neraiya vedios pannuinga biochemistry related pls bro i am waiting
Sir i am from ramanathapuram, and your teaching is very good and easily understand able and now i am getting clarifying my doubt's by seeing your videos ... Thank you sir
Eppo...epdiyo unga teachingla tha enaku selectable marker 2 conditions plus difference between transformants and non transformants purinchuchu....thanks a lot sir.....❤️
Super Senthilnathan Sir. Thank you very much SIR. Your method of Teaching is very very nice I like 4 persons Teaching very much. You are among one. Thank you very much Sir.
sir in the method of selectable marker....if we placed the transformant in antibiotics, it would not grow in the medium and it will die...So how we will use the plasmid further?🤔
We grow thousands of bacteria which contain foreign DNA in 2 separate medi a, one with ampicillin another medium with tetracycline, in which one medium will allow bacteria to grow another medium won't. So we conclude we have transformants, if bacteria grow on both media that means there is no transformants😊
@@BiologySimplifiedTamil sir but for eg: if we ligate the foreign dna with tetracycline resistant gene ,it will lose tetracycline Resistance and it will die in tetracycline petridish so we can conclude that it is transformant! But after concluding with this experiment we are not able to use this...since it is died so how they are we using further???
That's what we prepare 2 simultaneous plate one with tetracycline another with ampicillin, we keep bacteria in both the plate, if they are transformants they won't grow in one plate (tetracycline) and will grow in another plate (ampicillin), and we don't change the living into another plate (from ampicillin to tetracycline) we continue the experiment with living bacteria (transformants).
@@BiologySimplifiedTamil sir.ninga solringala ..The one that grows in ampillicin will not transformed to tetracycline..Apo epdi namma foreign dna tetracycline ku sensitive ah epdi solla mudiyum 🤔
Sir but the blue colour and colourless will indicate is it recombinant or non recombinant and what is the difference between transformant and recombinant sir
Sir enaku transformants purunjuthu aana recombinants and non recombinants pathi neenga sollala sir. Transformants and recombinants ku irukara diffrerence and appadina enna nu solli oru video podunga sir please. 🙏🙏🙏
Sr transformants thaana 2 types ah non recombinant and recombinants ah divide aagum appo atha pathi inom clear ah sollunga transformants ampicillin la grow aaguma
I am from ap but I can understand Tamil this is the best channel for biology I found after searching for various channel thank u sir
It means a lot for me... Thank you... Keep watching😊😊✌️✌️✌️
I also from ap i have seeing explanation for my exams from my 11th standard to now 2nd year of my b.s.c degree thank you sir for explanation
Any UA-cam channel for physics and chemistry
Idhellam yaara unkitta keta yaara nee😅
Iam from Kerala and I also liked this one!
Sir if u were my biology teacher i would have scored 100/100, such a grt explanation sir🙏
Bro Satyama sama bro, sama ya nadathuringa ippa tha unga channel ha pakura👏👏👏👏
Oru request gatb exam oda portions ha nadathuga bro❤️❤️❤️❤️
Now i am crystal clear of this cloning vector concept❤️🌹
Sir ,I'm from Sri Lanka . it's very helpful for me in this quarantine time.👍👍✌️✌️
Botany nega Viedo potta nalla erukkum sir.. padikirathuku... Unga class nalla purithu sir padikka
Masha Allah! Very nice sir! You're **BIOLOGY KING *😇😇
Jai sri ram
Masha Allah♥️✨
Sir I'm from telangana urs Tamil is easy to understand and my friend recommended me. Really it's very helpful to understand and special thanks to u both. ✨
I was really scared about this cloning vector because I thought it was complicated so I didn't touch it for a long time but now it looks very simple thank u
Bro semma bro, what u r major bro........................
Neet and great teaching bro, seriously bro yea padikuro ethukaga namala itha padikuro ithu enga use aagum ithulla ethukaga varuthu yea ithulla sollurainganea theriyamma ethos padikka irukku so padikanumea nu irukku intha educational system ......................................bro sathiyama sollurea ipo tha intha cloningea super ra understand aairukku , 10000 of thanks bro
Neraiya vedios pannuinga biochemistry related pls bro i am waiting
Sir I'm from Kerala.....I watched a lot of videos none of them reallyy helped me but yours was great and got the concept easily ..thank you
A great level of understanding👍
Thanks pa😊🙏
This man needs to be appreciated 😭😭😭😭❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️❤️
Sir i am from ramanathapuram,
and your teaching is very good and easily understand able and now i am getting clarifying my doubt's by seeing your videos ... Thank you sir
Sir, you are giving clear explanation , i watched many videos but this video explains clearly, tq sir keep doing
Eppo...epdiyo unga teachingla tha enaku selectable marker 2 conditions plus difference between transformants and non transformants purinchuchu....thanks a lot sir.....❤️
ஐயா! நீங்க மிகவும் அருமையாக பாடம் எடுக்கிறீர்கள் மிக்க நன்றி....😍🥳
Adipoli... 😍😍Love from Kerala 💓💖
Me to
Sir ennaku unga teaching semmaya purithung sir .thanks a lot sir...👌👌👌👌
Romba thanks sir indha lesson kastam nenachen unga explain patha perugu romba easy iruku indha lesson 🙏🏻🙏🏻
Fantabulous explanation (Selectable marker) sir 👍
Super Senthilnathan Sir. Thank you very much SIR.
Your method of Teaching is very very nice
I like 4 persons Teaching very much.
You are among one.
Thank you very much Sir.
Super ....way of explanation is just mind blowing ❤️
veraaalvll la sirr !!!! ...semasemaaa ...rombaanalla understood....whooff ivlodhnaa cloning vector apdinuh irukuhh ...hehhe !!!...thankyuu so much sir!!!...neenga maatum poii neet eludhunaa toppper dhan ...hahha!!.
Sir unga videos cbse students ku romba helpfull ah iruku! Thanks sir ✌🏻
sir.....it is very easy to understand....u r really teaching in a simplified way...than you so much sir.....👌👌👌
Respected sir, you explained everything about plasmid within very short time.thank you so much and be continue sir.
great way , more simplified.. just makes me "wow" ; ) never knew this lesson would be such easier.
The best lecture on cloning vector is this sir,tq this is very helpful for me👍
Thank you very much sir ......
Great explanation!!!!!!
Was so helpful
I am scared about this cloning vector relly very helpfull and useful sir thank you so much sir❤
A great video! This video explains concept very clearly!👍
Sir biology simplified Tamil.,.engaluku romba usefull la iruku sir nd keep doing...🙂
Sir I'm from nagai
Your teaching is very super sir
I can understand the cloning vectors
Jii vera level explanation🔥🔥🔥
Sir you are much gifted in teaching. God bless you sir...
Sir,I understood this topic very well becoz of u sir 🙏
sir in the method of selectable marker....if we placed the transformant in antibiotics, it would not grow in the medium and it will die...So how we will use the plasmid further?🤔
We grow thousands of bacteria which contain foreign DNA in 2 separate medi a, one with ampicillin another medium with tetracycline, in which one medium will allow bacteria to grow another medium won't. So we conclude we have transformants, if bacteria grow on both media that means there is no transformants😊
@@BiologySimplifiedTamil sir but for eg: if we ligate the foreign dna with tetracycline resistant gene ,it will lose tetracycline Resistance and it will die in tetracycline petridish so we can conclude that it is transformant! But after concluding with this experiment we are not able to use this...since it is died so how they are we using further???
That's what we prepare 2 simultaneous plate one with tetracycline another with ampicillin, we keep bacteria in both the plate, if they are transformants they won't grow in one plate (tetracycline) and will grow in another plate (ampicillin), and we don't change the living into another plate (from ampicillin to tetracycline) we continue the experiment with living bacteria (transformants).
@@BiologySimplifiedTamil oh ok sir now i understood.... thanks a lot for answering😊
@@BiologySimplifiedTamil sir.ninga solringala ..The one that grows in ampillicin will not transformed to tetracycline..Apo epdi namma foreign dna tetracycline ku sensitive ah epdi solla mudiyum 🤔
Very useful one 👌👍... Thankyou sir!!
Thank you✌️👍😊
Vera level ji ninga na unga video thaa full lah pathu biology padichu neet yelutha pore
It is very useful for biology students ...great ....i remmend your videos to my students ...
Sir Vera level teaching
Go ahead sir
Very clear explanation no one can give detailed and clear cut explanation like u sir pls kindly upload more videos
இந்த வகுப்புக்கு நன்றி ஐயா!
Sir how ori controls copy number??
Sir Iam from Tamil Nadu u video is helpful me so tanks sir
Sir pbr 322 in tn book say 322 means no.of plasmid produce in laboratory
Excellent explanation sir.... Thnx a lot for your service...
Sir chromogenic agent na ennathu sir ? Plse reply sir
Spr sir....college students ku rompa helpful la irukuk
Very,very clarity explanation. Thank u sir.
Thank u so much sir..very clearly explained sir....neraya doubts irundhuchu ....u clarified. It . .🤩😍
Thanks a lot.😊✌️
Sir 2 sites layum foreign dna bind ana wat will happen sir
Ipo nadanthatge thaan antibiotic property irukathu ..but athu possible ah rendu site layu bind aaguma
sir vectors and plasmids clear differentiation thanga please
Sir suppose if the foreign dna binds to EcoRl site which is outside from the 2 selectable marker then it is transforment right? how do we will find?
@@pranavshreeram5339 if u put ecor1 enzyme then only ,that gene gets cleaved and foreign dna binds there
Sir,verithanamana sambavam pannitenga indha video.inne epdi ketalum vetti saaikarathuthan
thalaivar vera ragam ..🔥
Supperra purithu neenga nadathurathu antigen presentation pathi Oru vedio podunga
Viral vectors - caMV, gemini vector video pothunga please
Sir teach this chapter
Strategies for enhancement in food production
Pls
It would be helpful for me for neet
Sure✌️😊
@@BiologySimplifiedTamil
Tq sir
Teach as soon as possible
Pls sir
Tq
Sir the no.322 is the no. Of E.coli developed in thier laboratory
Part 2 epa potuvinga sir ... thank you so much sir ...🙏easy ya puriya vaichitinga
Thank you sir , I understood well
Sit both tetracycline and ampicillin site la foreign DNA bind aana enna sir nadakkum
Amazing siir.......... nalla puurinchichiii sir
Sir apa nambha betagalactocidase ah vandhu oru cloning site ah pBR322 la use panurom ah sir
thank you so much sir......it was very useful ...i understood clearly!
Sir but the blue colour and colourless will indicate is it recombinant or non recombinant and what is the difference between transformant and recombinant sir
Hi da rp
Na hari da dei rp doubt la kelura mass da
Rhombha varshama puriyadha topic sir indha cloning site.rhombha nalla sonnenga 👍🙏
Super sir....Go ahead.......
Thank you sir✌️😊
Sir please upload cloning of dolly
Hello sir
I am appearing for NEET 2022
I had taken aadhar card when I was in 5th grade
Should I update my photo now in aadhar card sir 🤔
Sir your are amazing sir..... Please do videos on transgenic animals
Nalla understand aguthu sir.....more videos poduga sir
Sir rop is repressor of primer
3:44 Neenga solrathu apdiye coronaku solra Mathiri Irukku sir
sir starting la vara sound efects vara level to hear with head phones :)
Bro neega vera level keep going u reach your level best sir
Sir strategies involved in cloning vector naalum same thaana sir
Sir enaku transformants purunjuthu aana recombinants and non recombinants pathi neenga sollala sir. Transformants and recombinants ku irukara diffrerence and appadina enna nu solli oru video podunga sir please. 🙏🙏🙏
sir why we are not telling as insertional inactivation in insertion of foriegn dna in ampicillin and tetracycline cloning sites ?
Sir I have confusion about "BR" what is it's function like "R" is plasmid than what it's function of"BR"
Sir selectable marker use pannurathu oru destructive process but yen entha method a use pannuroam to identify the transformed cell?
IS E COLI AND PBR322 are same???
நன்றி
Sir genotic code nadathukuka sir pls 🙏
ligation adaptor and linkers
explain about the details sir
sir, discoveries, years,scientists name edhu allam oru video podunga sir ..easy ya errukum...pls..
Sure soon i upload
@@BiologySimplifiedTamil thanks sir...🙏
Simple and easily understanding explanation 🙃🙃
First artificial cloning vector is pSC 101 dhaana sir
Sr transformants thaana 2 types ah non recombinant and recombinants ah divide aagum appo atha pathi inom clear ah sollunga transformants ampicillin la grow aaguma
Sir antibiotic resistance Genes which is present in pbr322 act against antibiotics like ampicillin and tetracycline or other microorganisms like virus
Nice explanation sir . Excellent👍
Super sir
Thank you so much
Bro namba en oru bacteria ku antibiotic resistance gene kudukanum...
Sir can you please do video on puc18
Wowwww super explaination
Super sir you next God for us
Thank you so much sir ......
Welcome✌️😊
I have one doubt . recombinant term is similar to transformant.
Yes
But ,in my opinion,transformant is a host cell which received r-dna plasmid🙂