How To Calcium Test In Laboratory? o-CPC Method
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- Опубліковано 8 лют 2025
- This video describes the procedure for performing the CALCIUM CPC Reagent Test from Serum sample by Anamol Laboratories Private Limited, India.
Watch how to program CALCIUM - • CALCIUM CPC Programmin...
🔴Introduction🔴
Calcium plays an active physiological role in bone mineralisation, neuromuscular excitability, muscle contraction, and blood coagulation. Hypocalcemia can result from chronic renal failure with hypoproteinemia and hyperphosphatemia, or hypoparathyroidism, or vitamin D deficiency (osteomalacia, rickets). The most common cases of hypercalcemia are associated with hyperparathyroidism, tumours and bone metastases, hyperthyroidism or vitamin D overdose. Calciuria has little practical value in the various diagnoses except for the renal tubulopathies. Calcium can be estimated in serum / plasma using o-Cresolphthalein Complexone method.
🔴Features:🔴
• O-Cresolphthlein Complexone Specific To Calcium
• Fe And Mg Intereference Eliminated
• Liquid Stable Reagents With Long Shelf Life
• Fast Result
• High Linearity
• Suitable For Fully Automated Chemistry Analyzer
🔴Specification🔴
• Methodology - o-CPC
• No. Of Reagents - Two Liquids Ready-To-Use
• Shelf Life - 24 Months
• Sample Volume Required - 20 µL
• Test Time Required - 5 Minutes
• Linearity - 15 mg/dL
• Automation - Enabled
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This video is perfectly filmed. Thankyou sir!
Thank you very much sir. Please do subscribe to our channel and share this video with your friends and colleagues!
Please what fluid did you use as serum here
Excellent sir.......
mahoday aapaka bahut bahut dhanyavaad
Thank you. Pls like and share this video and subscribe to our channel.
Can we use urine as a sample instead of serum with this kit?
Thanks sir it help me lot
Thank you very much sir. Please do subscribe to our channel and share this video with your friends and colleagues!
Thanks you
Was 11.7 mg /dl little higher?
Nice
Thanks for this video
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Thanks sir
Well explain sir☺
Thank you for watching video.
Why glass tubes are not used for calcium assay?
Generally improper washing of the glass leads to error in result. You can refer to our separate video on this topic - How to clean laboratory glasswares to learn more about it. However, we will make a separate video on this topic for better explain the error!
should i aspirat standard and blank in every simple or only first if i had 12 sample .? should i wash before every simple by D.water ? please answer me.
Dear Sir, standard and blank need to be run only first time before running the samples. It is best practice to rinse with D.W. between samples also.
My calcium cerium test is 10.5 and the lab ref range is 10.30. Is mine high?
Sir.... Yaha hame yeah pata Lagta hain ki Hamare body me calcium kite percent hain tell me
Yes Sir.
What is the name of the machine?
Biochemistry
Sir factor se kaise pta chlega volume ka kitna serum lenge
Madam, is video me humne explain kiya hai 1:44 seconds pe ki 1 ml working solution me 20 microlitre sample add karna hai. Apko ye information company ke Instructions for Use (Pack Insert ya Literature ya Kit Insert) me mil jaygi.
@@anamollaboratories thx
May I use this procedure for taking sample as bacteria. To find the amount of calcium in it.
Sir, this kit is intended to be used on human samples. However, you can try at your level using bacterial samples.
Nice brother
Thank you for watching video.
Blank n standard sirf firts bar hi karne se hota hai kiya? Bar bar calcium test karne ke liye blank n standard karna sahiye kiya
Dear Madam,
Calcium ka working solution jab hum banate hai to around 1 week tak hum usse store kar sakte hai at 2-8 degree celcius. Lekin jab hum usse room temperature pe use karte hai to uski stability compromise ho jati hai. Isliye hume daily ek baar Blank aur Standard run karna chaihye agar hamare paas daily samples aa rahe hai to.
Mei tov ye kahun ga ki ager aap ki lab ki ye cheezein constant hai tov 2 weeks kay baad aap ko standard lagana chahiyay.
1. If temperature of lab is constant.
2.if pipetting is constant,i mean 0 pippeting error.
3. If u use always new test tubes free from dust and contamination.
4.if ur analyser is precise and accurate.
If incubation time is constant.
6.if tips are contamination free.
Then u can use standard after 1 month.
But listen if above mentioned are not constant in ur lab then use standard in each batch.
Ofcourse u can use blank after one weak.no need to use blank in every batch of tests.
CPC ka full form kya he?
Incubate time pls
who many rupes teast
Piste kang tawhana ka di ka masabtan
Sir video daliye na sir
Result was Wrong... U need to Wash it with water b/w Blank,std. And test (specially for Cal.) For accurate result
Sir, it was a high sample.
Sir calcium result came high
Yes sir, it was a high sample.
mahoday aapaka bahut bahut dhanyavaad
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