Interpretation of HPLC reports for Hemoglobinopathies

In this video I have explained about Interpretation of HPLC reports for Hemoglobinopathies in India
This video will be useful for all medical laboratory professional to interprete HPLC chromatograph and diagnose Hemoglobinopathies like sickle trait, sickle disease,beta thalassemia, HbE trait and disease, Hb D Punjab ( Heterozygous and Homozygous) , Hb lepore, HbJ Meerut, Hb Q India, HPFH and Double Heterozygous conditions in India
It took me three years to learn all different hemoglobinopathies which is covered in just 22 minute video !
#hplcreportinterpretation
#hplc
#hemoglobinopathies
#hplcforhemoglobinopathies
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TIMELINE FOR HPLC CHROMATOGRAPH INTERPRETATION VIDEO :
00:00 Introduction to HPLC chromatograph interpretation
00:11 Brief principle of HPLC
00:52 Common Hemoglobinopathies in India
02:01 Alpha gene variants
02:30 Beta gene variants
03:52 Biorad variant windows
04:03 Strategies to be followed to diagnose Hemoglobinopathies
05:08 Things to look after in chromatograph
06:45 HbA2 distribution
(Based on concentration different Hemoglobinopathies identified)
07:48 Factors /Diseases affecting HbA2
09:11 Chromatograph of beta thalassemia trait
10:08 Chromatograph of sickle cell trait
10:37 Chromatograph of sickle cell disease
11:17 Chromatograph of HbE trait
11:43 Chromatograph of HbE disease
12:05 Chromatograph of HbD Heterozygous (HbD Punjab)
12:59 Chromatograph of HbD Iran
13:52 Chromatograph of HbQ India
14:30 Chromatograph of Hb Lepore
15:32 Chromatograph of HbJ Meerut
16:21 Chromatograph of HbC trait
16:42 Windows wise Hemoglobinopathies (worldwide)
17:41 Examples of double Heterozygous
conditions
19:19 HPFH homozygous / Beta thalassemia major
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Video Highlights
What is normal range of HPLC?
What is HPLC?
How thalassemia diagnosed in HPLC?
What does it mean if HbA2 is high?
What if HbA2 is low?
Hemoglobin pattern analysis
Thalessemia and Hemoglobinopathies testing by HPLC
Detection of abnormal hemoglobin variants by HPLC methods

[@livebiochemistry]

TIMELINE FOR HPLC CHROMATOGRAPH INTERPRETATION VIDEO :
00:00 Introduction to HPLC chromatograph interpretation
00:11 Brief principle of HPLC
00:52 Common Hemoglobinopathies in India
02:01 Alpha gene variants
02:30 Beta gene variants
03:52 Biorad variant windows
04:03 Strategies to be followed to diagnose Hemoglobinopathies
05:08 Things to look after in chromatograph
06:45 HbA2 distribution
(Based on concentration different Hemoglobinopathies identified)
07:48 Factors /Diseases affecting HbA2
09:11 Chromatograph of beta thalassemia trait
10:08 Chromatograph of sickle cell trait
10:37 Chromatograph of sickle cell disease
11:17 Chromatograph of HbE trait
11:43 Chromatograph of HbE disease
12:05 Chromatograph of HbD Heterozygous (HbD Punjab)
12:59 Chromatograph of HbD Iran
13:52 Chromatograph of HbQ India
14:30 Chromatograph of Hb Lepore
15:32 Chromatograph of HbJ Meerut
16:21 Chromatograph of HbC trait
16:42 Windows wise Hemoglobinopathies (worldwide)
17:41 Examples of double Heterozygous conditions
19:19 HPFH homozygous / Beta thalassemia major

[@saadadaddad]

extremely helpful knowledge gaining session Dr Ashish.. thanks 👍

[@livebiochemistry]

Thanks Dr Sohil

[@margitgajjar9148]

Excellent Dr. Ashish... 👏

[@livebiochemistry]

Thank you dr Margit for comment and appreciation 👍🙏

[@skabdussalam7663]

Thank you sir for your excellent vedio.

[@livebiochemistry]

Thank you for comment 🙏

[@user-uk5gb2xo6p]

Great explanation, thanks

[@livebiochemistry]

Glad it was helpful!

[@GubbuDubooChotuKanhaa]

❤❤❤❤

[@livebiochemistry]

Thank you for comment

[@farhankhanzada855]

Excellent sir

[@livebiochemistry]

Tx for appreciation

[@abhiriseagain9303]

Thank you so much sir.

[@livebiochemistry]

Glad it is helpful to you
Tx for comment

[@snehalchavan8227]

Good information. Thank you.

[@livebiochemistry]

Thanks for your comment !!

[@cookie5335]

A little off topic.... Can u cover parasites and tests u can do to detect microscopic parasites.
I have animals and I feel I could have parasites I'd like to go to the Dr and ask for proper test to be done.
If u can do that it would be great. Thank you very much🙂

[@livebiochemistry]

Yes surely ... laboratory microscopy to be covered soon

[@sapnadamor1581]

Sir if s window comes less than 20, then report is normal or we have to repeat or do anything else?

[@livebiochemistry]

Lab should go for other test ..like sickling.../solubility test. .how much exact value got S window. .15-40 will be Trait. Also history of blood transfusion in last 3 month to be rule out.

[@user-xh2zb8fc2s]

Can you please share the pump gradient for poly cat column?

[@livebiochemistry]

We are not using it .

[@AbhishekPal-ds8rn]

Sir, I am mlt student, please guide me how prepare for msc biochemistry for aiims exam. 🙏🙏

[@livebiochemistry]

AIIMS msc biochemistry exam : Patten -single best answer mcq
Topic : biochemistry, molecular biology and immunology MCQs only
You could refer rebecaa James mcq biochemistry book or biochemistry by Karthikeyan pethuswamy book

[@AbhishekPal-ds8rn]

@@livebiochemistry Thank u so much sir g 🙏🙏

[@livebiochemistry]

Welcome..best of luck

[@niteshvaishnav8072]

Mera hbs 62% hai aur hplc report mein likha hai..compound heterozygous conditions of hemoglobin s iska matlab Kya hai sir??

[@livebiochemistry]

How much HbA2 or HbF ? can you send your CBC and HPLC report on [e-mail]

[@Sumankayal1995]

Hi, recently did HPLC test... These are findings
Hb A2 : 3.7%
Hb F : 0.8%
Hb A: 95.5%
Haemoglobin: 17.2
MCV: 96.7
MCH: 31.1
RBC : 5.5
RDW-CV : 18.3
Could you please suggest if this is beta thalassemia carrier or any other test is required to confirm

[@livebiochemistry]

No...cbc reports and HPLC finding rule out thal minor / thal carrier ...it's normal

[@1118roops]

How to report borderline or intermediate HbA2? 3.6 - 4.0

[@livebiochemistry]

Looking for other parameters like RBC count ( which will be high ) MCV and RDW ( RDW not much high ) ...s iron .Hb ..etc
Also to rule out Hyperthyroidism and megaloblastic anaemia
Ans report final confirmation with DNA Sequencing ( Hbb sequencing

[@debmalyasarkar875]

Sir, HPLC haem D-10 Bio rad test report
HB A0 83.8% Result
It is darkened deep black.
Is there any problem or chance of Thalassemia. Pls comment. Thnx in advance.🙏

[@livebiochemistry]

Please send HPLC report and CBC report on [e-mail]

[@aldsrb8933]

Hba2 1.7 hai ye severe hai kya ,DNA reporrt last year normal tha

[@livebiochemistry]

Can you send your CBC report and DNA report along with details age/sex . community...any other reports ( like iron tibc ferritin, ) on
[e-mail]
1.7 is little lower....( 2-3.5 normalmay occur in iron deficiency anaemIa too .

[@abhipallavitripathi6194]

Can a thalsemia minor donate blood to normal person?

[@livebiochemistry]

Very nice question...they can donate blood provided they fullfill hemoglobin criteria !

[@knowledgeispower818]

Sir please expalin HB D disease? Its major or minor?

[@livebiochemistry]

HbD major mild hemolytic anaemia
HbD minor as such asymptpmatic
Problem when partner is beta thal minor and child have possibility of major disease

[@vikrambhati02]

Sir yah book konsi h

[@livebiochemistry]

Which one ?

[@italiantourdu]

My hba2 is 2,3 is this ok?

[@livebiochemistry]

Yes it's fine .can you send CBC reports and other lab reports if performed to [e-mail]
As in Iron def anaemia HbA2 may be low..

[@italiantourdu]

Thank u so much sir.u release my tension other reports are. Normal including CMV etc.

[@SONU474]

Dear sir my baby 2.5 years female hplc report
Hba 82% hba2 2.4%
HbF 4.6 % Konsa
Theleshimia hai
Minor ?
Major ?
Intermedia ?
Mcv 87
Mchc36
Pcv 27
RBC 3
WBC 8000

[@livebiochemistry]

Please send complete CBC report and HPLC report on [e-mail]
Also any complains ? History of blood transfusion ?

[@SONU474]

I have already send cbc with hplc report your email address

[@livebiochemistry]

@@SONU474 any history of blood transfusion .how much time in recent times