@OldScoolChemistry AMAZING... please explain why you are so straightforward with your explanation, but others beat around the bush like it's their job? just subscribed, keep this up!
Ma'am can you make a video on this topic."Determination of available oxygen and manganese dioxide in pyrolusite ore by KMnO4". Because I can't a proper video no UA-cam
Is that posible to record absorbance data greater than 1 during OD reading in spectrophotometer? because lambert beer law describes that absorbance greater than 1 can not give you straight line.
The absorbance is read on the spectrophotometer. It comes from the wavelength of the light absorbed which the machine reads as transmittance, and then is calculated to absorbance. Maybe I misunderstood your question. You may want to ask your question to your professor so they can clarify. Thanks!
@@OldSchoolChemistry, I thank you for your effort in making a contribution to knowledge that transforms humanity. As part of my PhD project, I was looking to identify an ester in some feed compound. I learned that it is possible to identify the presence of ester in organic samples through Ferric Hydroxamate Test. This test only shows the presence/absence of ester in your sample. It does not tell you the amount (concentration). That's where I came to know your channel. What if I measure the absorbance of my samples using a spectrophotometer and calculate the concentration of the available esters in my sample?
Hi Mohamed, This is the only way with spectrophotometry. Remember once you have the plot you can simply use two points in the line to calculate concentration. You don’t actually have to find the equation of the line. I hope chemistry goes well for you. Please subscribe if you have not already. Thanks!😊
@@OldSchoolChemistry thanks for replying but I meant way other than spectrophotometer Something that can done in simple lap without many equipments Great video btw 😊
Hi p h, wavelength is the distance between of a crest and crest of one wave of light. Wavelength is specific to the color of your solution. Absorbance, in essence, is the amount of light absorbed by the solution. The spectrophotometer is set to a wavelength to detect the amount of light absorbed by a sample at that specific wavelength. I hope that helps!!
Hi Su zeO, no. This is because the concentration on the x axis was calculated considering the dilution factor. The concentration on the x axis should accurately represent the actual concentration put into the spectrophotometer machine. I hope that helps. Thanks!
Best chemistry channel on UA-cam by far for concise explanations . Forget CGI , old school by a professional chemist and educator is best.
Hey Barask, you are so kind. I appreciate your great support -thanks! I hope all is well in your neck of the woods.🙂
@@OldSchoolChemistry 9.57k onwards and upwards. I am currently using Bing creative commons images to illustrate the book.
@@baraskparas9559 That is fantastic! Send me a link to the book when you are finished:) Way to go!
Thank you. This is all I needed to know in one video.
HI Britney, I am so glad! Thank you for your comment:)
Wow just wow. Absolutely Great teaching
Hi Aywan, I’m so glad, thanks! If you haven’t already, please subscribe. I hope your class goes well😊
Thank you. You are my newest best friend and you don't even know it. Your teaching is excellent.
Hi Trohn, that makes me so happy...new friend;) Thanks!
Plz plz keep it up mam never stop making vedios helps helps us very much a veiwer from india
Hi Shivam, thank you. I hope your class goes well. Please subscribe if you have not already!😊
@OldScoolChemistry AMAZING... please explain why you are so straightforward with your explanation, but others beat around the bush like it's their job? just subscribed, keep this up!
Hi Vincent, I am glad you find it straight forward! Thanks for subscribing:)
I just subscribed love your explanation!
Hi Islandgirlzzs, THANK YOU! I am so glad you liked the video:)
Thank you so much ma'am for making this video
Really this is very helpful to me to understand.
Imaginative Images, I’m so glad this was helpful. I hope your class goes well!!😊
Ma'am can you make a video on this topic."Determination of available
oxygen and manganese dioxide in
pyrolusite ore by KMnO4". Because I can't a proper video no UA-cam
@@kalivarapupavan6228 Thank you for the recommendation. Good luck!
Wow, I understand very much. Thank you
Wonderful! That makes me so happy. Well done:)
THank you for your clear and very helpful videos!! :)
Hi Gaby, thanks for your kind feedback. You are welcome. I hope your class goes well!😊
i am also an educator, nice explanation and easy to learn
Hi Louege, Thank you so much for that kind comment. Thank you also for your work with students! God bless you:)
Is that posible to record absorbance data greater than 1 during OD reading in spectrophotometer? because lambert beer law describes that absorbance greater than 1 can not give you straight line.
The absorbance is read on the spectrophotometer. It comes from the wavelength of the light absorbed which the machine reads as transmittance, and then is calculated to absorbance. Maybe I misunderstood your question. You may want to ask your question to your professor so they can clarify. Thanks!
This world is not fair. 6.37K followers for this channel is unfair. It should have been something like 6.37M.
Thank you chernetwoju6284!!💗
@@OldSchoolChemistry, I thank you for your effort in making a contribution to knowledge that transforms humanity.
As part of my PhD project, I was looking to identify an ester in some feed compound. I learned that it is possible to identify the presence of ester in organic samples through Ferric Hydroxamate Test. This test only shows the presence/absence of ester in your sample. It does not tell you the amount (concentration). That's where I came to know your channel. What if I measure the absorbance of my samples using a spectrophotometer and calculate the concentration of the available esters in my sample?
@@chernetwoju4306 Wow! That sounds awesome. Creative idea to use spectrophotometry. I hope it works! Good job:)
is there other maybe more easy ways to calculate concentration of unknown?
Hi Mohamed, This is the only way with spectrophotometry. Remember once you have the plot you can simply use two points in the line to calculate concentration. You don’t actually have to find the equation of the line. I hope chemistry goes well for you. Please subscribe if you have not already. Thanks!😊
@@OldSchoolChemistry thanks for replying but I meant way other than spectrophotometer
Something that can done in simple lap without many equipments
Great video btw 😊
Possibly a gravimetric analysis with a precipitation reaction depending in the solute.
in my work it asks for Wavelength and Absorbance can you explain what each means and how they connect with each other? Thank you
Hi p h, wavelength is the distance between of a crest and crest of one wave of light. Wavelength is specific to the color of your solution. Absorbance, in essence, is the amount of light absorbed by the solution. The spectrophotometer is set to a wavelength to detect the amount of light absorbed by a sample at that specific wavelength. I hope that helps!!
thank you🌸
Hi Silatopia, You are so welcome!! I hope you class goes well:)
Once I have determined the conc. of the unknown sample using the curve, do I need to multiply it by the dilution factor ?
Hi Su zeO, no. This is because the concentration on the x axis was calculated considering the dilution factor. The concentration on the x axis should accurately represent the actual concentration put into the spectrophotometer machine. I hope that helps. Thanks!
Thank you 🙏🏻
Hi Diki, thank you for your comment ❤️
hey thanks
Hi Vrushabh, your welcome:)