I think I have the same question. Is it one transposase and therefore barcode type per nucleus (i.e. only one transposase can infiltrate one nucleus)? Or does the nucleus remain intact so that all the transposases and their different barcodes contained within a single nucleus are isolated?
The transposase itself doesn't carry the barcode, the barcode is carried by the primers within each gel droplet (hence diff color of diff droplet). Thus, transposed nucleus (which has tons of Tn5 at that point) remain intact and will only be barcoded and amplified upon successful emulsion.
![[WEBINAR] Understanding Single-Cell ATAC-Seq and its Applications](/img/n.gif)
Amazing lecture thank you so much
so lucky that I found your channel!!!
Amazing lecture, thank you so much for uploading your excellent course
I think I have the same question. Is it one transposase and therefore barcode type per nucleus (i.e. only one transposase can infiltrate one nucleus)? Or does the nucleus remain intact so that all the transposases and their different barcodes contained within a single nucleus are isolated?
The transposase itself doesn't carry the barcode, the barcode is carried by the primers within each gel droplet (hence diff color of diff droplet). Thus, transposed nucleus (which has tons of Tn5 at that point) remain intact and will only be barcoded and amplified upon successful emulsion.
Can you use this on whole drosophila tissue eg the brain?
Yes!
Is it necessary to do RNA seq before ATAC seq?