Відео

Thanks!

Thank you for the nice comment and glad you liked the video. This is a pretty cool rotifer!

Thanks for your interesting comment! As you probably know, before phase contrast, oblique illumination was the most useful way to increase contrast in material of low contrast and condenser holders were designed to facilitate oblique illumination such that you could move the condenser horizontally to achieve oblique illumination. I would be interested to learn more about the details of your combination of oblique illumination and polarization. BTW, I lucked out big time in being able to obtain a microscope equipped with DIC optics on eBay for a very reasonable price because, I think, the seller did not really know the value of what they were selling.

@tjlmicro6508 By offsetting a wedged shaped stop in the filter holder, using high light intensity and adjusting polarization I can get the 3D effect along with good contrast of protist's interior. It appears very much like the clips of DIC online. What scope did you get the great deal on?

@@rcmjones Thanks for the information. Here is some information on my microscope: www.microbehunter.com/microscopy-forum/viewtopic.php?f=24&t=14783

Thanks! Now I need to find another one

Hi Don. Not sure I understand your comments. Could you clarify, please?

@@tjlmicro6508 Perhaps I did understand better than I first thought. There is a bridge composed of connecting plasma materials. The bridge connects two cells. Is that it? Sorry, it seems clear now on second look.

@@DonSchaeffer Hi Don, no problem. These are interesting organisms in which individual heliozoans form colonies that are connected together by these bridges. Sometimes these can large numbers of individuals in each colony. I did not know that these connected individuals are really connected to one another and can exchange vesicles between them. I have tried to find some additional information about this but have not been able to find anything. I hope this additional information is useful. Tim

@@tjlmicro6508 I did feel that understandable information was hard to find. Thanks for this.

1-2 mm so you can see them with the naked eye

@@tjlmicro6508 Wow!

The microscope is a Zeiss Photomicroscope III equipped with DIC optics. The objective was a 16x planachromat. The camera was a Canon 1300D attached to an Amscope adapter. The video was captured using the Canon EOS Utility software.

The video was taken with a Zeiss Photomicroscope III equipped for DIC. A Plan 40x objective was used and the optovar was set at 2x. The camera was a Canon 1300D with an Amscope camera adaptor to allow the camera to be attached to the phototube on the microscope. The camera was set on Movie mode and the video was captured in Live View using EOS Utility software.