Featuring: Chad Deisenroth, PhD and Kostja Renko, Dr. rer. Nat.
Kostja Renko, Dr. rer. Nat., presents "Identification of potential thyroid hormone disruptors using the Sandell-Kolthoff reaction as a technology platform."
ABSTRACT: The Sandell-Kolthoff (SK) reaction, as a "classical" analytical tool for the detection and quantification of minute amounts of iodine, has recently gained interest through its increasing use as a readout for in vitro methods in the thyroid field. As many processes and key functions of the thyroid axis involve the transport or metabolism of iodine-containing molecules, the SK reaction has emerged as a useful and easy-to-use readout for several assays that reflect crucial key functions/molecular initiating events of the hormonal axis, including TH metabolising deiodinases, TH transport via transmembrane transporters (e.g. MCT8) or activity of the sodium/iodide symporter. The webinar will give an overview of the applications and the potential of using a unified SK setup as a central platform to perform in vitro tests and ex vivo analysis.
Dr. Renko is a scientist at the German Federal Institute for Risk Assessment in the unit "Strategies for Toxicological Assessments." As a biotechnologist, he works on in vitro methods for the identification and characterization of potential thyroid disrupting chemicals.
Chad Deisenroth, PhD, presents "Advancing Translational Application and Acceptance of The 3D Human Thyroid Microtissue Assay"
ABSTRACT: The human thyroid microtissue (hTMT) assay utilizes primary human thyrocytes to reproduce structural and functional features of the thyroid gland to enable testing for potential thyroid-disrupting chemicals. As a variable-donor assay platform, conventional principles for assay performance standardization need to be balanced with the ability to predict a range of human responses. This presentation will highlight current efforts to evaluate biological and toxicological variability in the model to establish criteria for donor thyrocyte qualification and performance guidelines for method validation.
Dr. Deisenroth is a cell biologist in the Center for Computational Toxicology and Exposure at the U.S. EPA with 14 years of experience in the development and application of in vitro new approach methods for predictive toxicology.
Recordings and other materials from this webinar will be posted on the ASCCT webinar archive: www.ascctox.org/webinar-archive