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RBR Life Science
India
Приєднався 7 сер 2021
Welcome all ,
RBR Life Science is a free education platform for all life science / Biology Students . Myself RBR , MSc Zoology , CSIR NET 2015, CSIR NET 2017, SET (Maharashtra) -2019 Qualified & I am researcher in Biotechnology .This Channel offer free online biology lectures covering multiple biology fields like Marine Biology , Biotechnology , Zoology , Botany, Microbiology , Virology & Chemistry right from class 10 , Class 11, Class12, Undergraduate BSc courses in Lifesciences , MSc Life Science , and Medical Degree Students. We also provide online video content of all Competitive Exams such as SET ,NET GATE Exams, current affairs , General Aptitude , Exam Pattern, Strategy for various exams , Career Guidance for aspirants of different Educational streams such as Arts, Commerce , Science . Encourage me to grow this free educational channel with your support and subscription.
Thank You
RBR Life Science is a free education platform for all life science / Biology Students . Myself RBR , MSc Zoology , CSIR NET 2015, CSIR NET 2017, SET (Maharashtra) -2019 Qualified & I am researcher in Biotechnology .This Channel offer free online biology lectures covering multiple biology fields like Marine Biology , Biotechnology , Zoology , Botany, Microbiology , Virology & Chemistry right from class 10 , Class 11, Class12, Undergraduate BSc courses in Lifesciences , MSc Life Science , and Medical Degree Students. We also provide online video content of all Competitive Exams such as SET ,NET GATE Exams, current affairs , General Aptitude , Exam Pattern, Strategy for various exams , Career Guidance for aspirants of different Educational streams such as Arts, Commerce , Science . Encourage me to grow this free educational channel with your support and subscription.
Thank You
Agar-Agar in Microbiology | Agar-Agar | Agar Powder
this video explains what is agar-agar in microbiology media. and the composition and uses of agar-agar powder. Agar powder is a gelatinous substance of marine red algae used in microbiology media and it is a very good solidifying agent.
visit my blog for more details.
rbrlifescience.com
Hi I am RBR, Welcome to our youtube channel RBR Life science
About this video-
friends, In this video tutorial we are going to discuss what is agar-agar
Queries solved:
1. What is agar-agar
2. What is agar powder used in microbiology?
3. What is the purpose of agar powder in culture media?
4. What are the different ingredients present in agar- agar powder
5. What is the composition of agar-agar?
6. What are the uses of agar powder
#what is agar-agar in microbiology
#agar-agar
#agar powder composition
#agar-agar uses
#agar powder receipe
#microbiologyagar
#agar-agar
#agar-agar in micobiology
#agar powder
#agar agar powder
#what is agar agar powder
visit my blog for more details.
rbrlifescience.com
Hi I am RBR, Welcome to our youtube channel RBR Life science
About this video-
friends, In this video tutorial we are going to discuss what is agar-agar
Queries solved:
1. What is agar-agar
2. What is agar powder used in microbiology?
3. What is the purpose of agar powder in culture media?
4. What are the different ingredients present in agar- agar powder
5. What is the composition of agar-agar?
6. What are the uses of agar powder
#what is agar-agar in microbiology
#agar-agar
#agar powder composition
#agar-agar uses
#agar powder receipe
#microbiologyagar
#agar-agar
#agar-agar in micobiology
#agar powder
#agar agar powder
#what is agar agar powder
Переглядів: 3 845
Відео
Mannitol Salt Agar | MSA
Переглядів 8 тис.Рік тому
In this video, we're going to be talking about Mannitol salt Agar media, and its principle, composition, uses. This is a very important culture media, as it allows us to grow and identify microorganisms that utilize mannitol If you're looking to learn more about the different uses for mannitol salt Agar, or want to understand how it works, then this is the video for you! We'll be discussing the...
MacConkey Agar | Composition of McConkey agar
Переглядів 26 тис.Рік тому
In this video, we're going to be talking about MacConkey Agar media, and its principle, composition, uses. This is a very important culture media , as it allows us to grow and identify microorganisms on which utilize lactose If you're looking to learn more about the different uses for Macconkey Agar, or want to understand how it works, then this is the video for you! We'll be discussing the dif...
what is nutirent agar | Nutrient agar microbiology
Переглядів 25 тис.Рік тому
In this video, we're going to be talking about what is Nutrient Agar, and what it is used for. This is a very important topic, as it allows us to grow and identify microorganisms on Nutrient Agar, which is important for microbiology. If you're looking to learn more about the different uses for Nutrient Agar, or want to understand how it works, then this is the video for you! We'll be discussing...
Nutrient Broth Media Preparation | How to prepare nutrient broth media
Переглядів 39 тис.2 роки тому
In microbiology, it is very important to know the procedure of Nutrient Broth Media Preparation and how to prepare nutrient broth media for routine microbiological work. Nutrient broth is a liquid culture medium used to grow microorganisms, especially bacteria. Visit blog for more details: rbrlifescience.com Hi, I Am Rahul B Rathod, Welcome to Our You Tube Channel RBR Life Science About this vi...
what is nutrient broth
Переглядів 9 тис.2 роки тому
The focus of this video is what is nutrient broth, and the composition and uses of nutrient broth. The nutrient broth is a liquid culture medium used in microbiology for growing microorganisms such as bacteria. About this video- friends, In this video tutorial we are going to discuss what is nutrient broth medium Queries solved: 1. What is a nutrient broth solution? 2. What is nutrient broth us...
What is Acid Fast Bacteria | Everything about Gram +ve , Gram -ve & Acid Fast Bacteria
Переглядів 14 тис.2 роки тому
This video demonstrates structural difference between Gram Positive, Gram Negative and Acid fast bacteria. Acid Fast Staining Procedure is a differential staining procedure used to differentiate bacteria into acid-fast and non-acid-fast bacteria. acid-fast bacteria have a waxy cell wall made from complex hydrocarbon known as mycolic acid. This difference in cell wall composition makes bacterial...
Acid Fast Staining Procedure
Переглядів 33 тис.2 роки тому
This video demonstrates the ACID FAST STAINING technique. Acid Fast Staining Procedure is a differential staining procedure used to differentiate bacteria into acid-fast and non-acid-fast bacteria. acid-fast bacteria have a waxy cell wall made from complex hydrocarbon known as mycolic acid. This difference in cell wall composition makes bacterial cells react differently to acid-fast stains. Aft...
Gram Staining Procedure
Переглядів 86 тис.2 роки тому
Gram Staining Procedure is a differential staining procedure used to differentiate bacteria into two separate groups such as gram-positive bacteria and gram negative bacteria. The difference in cell wall composition makes bacterial cells react differently to gram stain. After a successful gram staining procedure, Gram-positive bacteria appear purple in color and Gram-negative bacteria appear Pi...
Radiant streak method of isolation | Microbiology Part 6
Переглядів 7 тис.2 роки тому
the radiant streak plate method is a technique of isolation of pure culture of the microorganisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in a radial direction so that bacterial cell density gets diluted and we get isolated colonies. Visit blog for more details: rbrlifescience.com Hi I Am Rahul Rathod. Welcome to Our You Tube Channel RBR Life S...
continuous streak plate method | part 5
Переглядів 7 тис.2 роки тому
The continuous streak plate method is a technique of isolation of pure culture of the microorganisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in such a way that bacterial cell density gets diluted and we get isolated colonies. Visit blog for more details: rbrlifescience.com Hi I Am Rahul Rathod. Welcome to Our You Tube Channel RBR Life Science. ...
Zigzag streaking method of isolation | Microbiology | Part 4
Переглядів 6 тис.2 роки тому
Zigzag streak plate method is a technique of inoculation of pure culture of the microorganisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in such a way that bacterial cell density gets diluted and we get isolated single colonies. Visit blog for more details: rbrlifescience.com Hi I Am Rahul Rathod. Welcome to Our You Tube Channel RBR Life Science....
Four Quadrant streak method of isolation | Part 3
Переглядів 18 тис.2 роки тому
Four quadrant streak plate method is a technique of isolation of pure culture of the microorganisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in such a way that bacterial cell density gets diluted and we get isolated single colonies. Visit blog for more details: rbrlifescience.com Hi I Am Rahul Rathod. Welcome to Our You Tube Channel RBR Life Sci...
T streak plate method of isolation | Part 2
Переглядів 16 тис.2 роки тому
T Streak plate method of isolation is a technique of isolation of pure culture of the microorganisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in three sections in such a way that bacterial cell density gets diluted and we get isolated colonies. Visit blog for more details: rbrlifescience.com Hi I Am Rahul Rathod. Welcome to Our You Tube Channel ...
Streak plate method | Pure culture technique Microbiology | Part 1
Переглядів 31 тис.2 роки тому
Streak plate method is a technique of isolation of pure culture of the microorganisms (mostly bacteria), from a mixed population. The inoculum is streaked over the agar surface in such a way that bacterial cell density gets diluted and we get isolated colonies. Visit blog for more details: rbrlifescience.com Hi I Am Rahul Rathod. Welcome to Our You Tube Channel RBR Life Science. About this vide...
Catabolite Repression | cAMP, CAP, Glucose and Lac Operon.
Переглядів 24 тис.2 роки тому
Catabolite Repression | cAMP, CAP, Glucose and Lac Operon.
Calculate Colony Forming Units | Microbiolgy CFU/ml Count
Переглядів 27 тис.2 роки тому
Calculate Colony Forming Units | Microbiolgy CFU/ml Count
Lac operon & lactose metabolism l Gene regulation in E.Coli
Переглядів 1,5 тис.2 роки тому
Lac operon & lactose metabolism l Gene regulation in E.Coli
Preparation of nutrient agar plates l How to make agar plate
Переглядів 172 тис.2 роки тому
Preparation of nutrient agar plates l How to make agar plate
Serial Dilution method | viable cell count method
Переглядів 104 тис.3 роки тому
Serial Dilution method | viable cell count method
thank you sir for this detailed explanation!
Thank you and stay tuned
impeccable editing and superb explanation. love your content!
Welcome & thank you for compliments.
thank you!
Welcome
Why 1grm of soil in 50ml water?. Is dis jt an assumption or it's from an SOP?
Generally soil contains organic materials so it will have large number of bacteria. To get isolated colonies it is better to take 1 gm of soil. It's an assumption. It's not as per SOP
Thank you sir
Thanks Sarita 😀
thankyou sir
Welcome Sneha
dirty punjabi
Is it necessary for antibacterial activity
if 13gram is for 1000mL , why you only added 500mL ??
I have taken 1.5 grams of Nutrient broth powder in a 500 ml capacity flask and in this 500 ml capacity flask I have added 100 ml distilled water. Always make media in Higher capacity flask. For example If you are making 100 ml NB then prepare it in 500 ml capacity flask. It will also leave head space during autoclave and space for oxygen if bacteria are inoculated directly in flask.
@@rbrlifescience Thankyou sir. i am new to microbiology so a little knowledge. can u help me in one question..suppose i have to do graim staining for that i need a sample of bacteria to be fixed on glass.. Where will i get this bacteria from ? i dont have any cultured bacteria
Tq sir
Welcome
well explained
Thank you So much and Stay Tuned
Is this method according to iso? I lately worked in a lab wich they consider an inoculum of 0.1 ml from dilution 1/10 as a1/100 dilution on Petri dish Is that right? I could not understand it because they only spread the inoculum & not diluting it. Please I want an answer.
Yes it is but you have to do literature survey
@@rbrlifescience Thank you for replying. But your yes belongs to which part of my question? Is spreading 0.1 ml of dilution 1/10 on a solid culture medium becomes 1/100? Would you please briefly explain it? Or give me a link or any reference?
microbenotes.com/serial-dilution/
@@rbrlifescience Thank you so much.
Hello sir
Hi
Wow
Welcome 🙂
Casi no entendí pero las imágenes ayudaron mucho para comprender y me siento mas seguro para hacer mi examen de esto
English please
Plz remove subtitle
Yes I agree bro but you can remove it from settings
Thank you. It was very educative. One question. Why would we need to find out the number of cells? In what research do we need it?
To see the growth pattern of bacteria in culture we need to count cells.
ii only get a mass of colonies in the first sector whenever i try these lol
With practice you will get isolated colonies
After preparing the Agar solution, can i use it to culture bacteria within 48 hrs without putting it in d refrigerator?
Yes you can use plates after 24 hrs of incubation
Nice
Most welcome
Thank you very much sir for this great explanation ❤
Most welcome
But why we use autoclaving or incubator
We use autoclave to setrilize and dissolve the media. After sterilization we pour the media in petri plate and after agar in plate solidifies we need to keep plates in incubator for drying for 24 hrs -48 hrs. This incubation is also check any contamination while pouring.
How do we know that we need 4.6gm of NA in 200ml of H2O
On NA bottle, it is mentioned that for making 1000 ml of NA you need to take 28 grams of NA powder from bottle and suspend it in 1000 ml distilled water. It's just calculation 28 g for 1000ml 2.8g for 100 ml 4.6g for 200 ml Have a great day 😊
Thank you sir very nice class🙏🙏
Welcome 🤗
very helpful:))
Welcome
More blessings from God always 🙏🙏
Thank you & Welcome sir
Thank you very much that you told me:
Welcome
The best explanation out there. you are awesome 💯❤ ❤
Thank you Dear
Informative love from West Bengal ❤️😊
Thank you
Good sir chala Baga Ardamautundhi inka petandi sir micro biology stains dmlt came to exam sir
Amazing!❤
Welcome
Good explain sir tq I'm Bvoc mlt student.
Welcome
sir, why unused sterile medium should be then stored in the fridge rather than at room temperature condition?
The moisture from the agar medium will evaporate, and plates will become dry. For microbial growth to occur we need water in the agar plate. Also if we keep the petri plates outside then there will be chances of microbial contamination.
So nice
so would a light orange ish color be negative for mannitol production
MSA contains a pH indicator, phenol red, that is red-orange when the pH is neutral (around pH 7). If a species ferments mannitol and produces acid, the pH of the medium decreases and phenol red will become yellow. medium remains red-orange after growth and growth observed: mannitol fermentation negative medium is yellow after growth: mannitol fermentation positive
Thank you sir
Welcome
Good
Thank you
❤❤❤
Welcome
Wow kamal ka ye banda hai ❤😊😊
Thank you Bhai
i loved it🥳🥳
Welcome
❤❤❤ ⚘️🌷⚘️...Thank you...🙏🙏😊😊😊👍👍👍
Most welcome
Thank you
Most welcome
Where is msc MLT college s Iam desable that way i hope that,, from home can i do msc mlt ,, any option ,, please send me reply sir
Mostly government medical colleges offer post graduate diploma in MLT which is called PG DMLT.
@@rbrlifescience now , wher that collage s ? But I did it Bsc MLT after DMLT, then pg DMLT equal to msc graduation.
Can I keep it for 48 hrs before refrigeration
Yes off course
I tried this Experiment but got no reasult Also my solution looks whitish
After autoclaving solution will be clear
@@rbrlifescience but the agar solution is yellowish In general My solution remains pale yellow even after autoclave & yes I am taking an edible agar (used to make jelly) is it OK?
Once agar in plate solidifies then it remains slightly yellowish. Nothing to worry about.
@@rbrlifescience it's not about the color the problem is I am not getting bacteria culture even after 7days
hi! what can we do if we’ll just sterilize the agar using pressure cooker? (we don’t have autoclave)
Yes it will sterilize the agar
Very helpful video
Welcome
Why do some nutrient gar need to add sodium for prep?
Thank you ❤
thank you very much. well understood