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video tutorials for scientific laboratory instruments
United States
Приєднався 28 січ 2014
This content is provided to help you become better at using the various instruments and software in your laboratory. Please subscribe and provide feedback or requests for content. Thank you!
Tecan HydroFlex Microplate washer -Getting Started - Basic Setup & Connections
This is just a video on how to connect liquid lines and cables to the Tecan HydroFlex washer once it is taken out of the box. This is NOT instruction for how to create wash protocols and perform microplate washing.
Переглядів: 897
Відео
Luminescence Measurements with Tecan Infinite 200Pro Microplate Readers and iControl Software
Переглядів 7 тис.4 роки тому
This is a brief introduction to the basics of collecting luminescence data using the photon counting detector of the Tecan Infinite 200Pro microplate readers using iControl software. Raw data is automatically sent to EXCEL. This is the link to Promega Dual Glo assays www.promega.com/products/luciferase-assays/reporter-assays/dual_glo-luciferase-assay-system/ This is a link for learning more abo...
Fluorescence Intensity Detection with Tecan Spark Microplate reader and SparkControl Software
Переглядів 15 тис.5 років тому
How to collect fluorescence intensity measurements with the Tecan Spark multimode microplate reader and SparkControl Software. Topics covered include: click on time stamps to jump to specific topics Plate selection @0:46 Selecting settings for Flashes @7:34 Wavelengths and Bandwidth, and following the Minimum Distance Rule @3:05 Gain @8:20 Z position Optimization @12:52 Multiple Reads Per Well ...
Tecan iControl Software - Well Wise Kinetic Measurement with Injectors
Переглядів 1,8 тис.6 років тому
This video covers the use of iControl software with Tecan Infinite microplate readers for Well-Wise kinetic measurements. It also gives an example of one of many possible ways to use injectors for initiating a reaction and measuring that reaction to completion within one well before repeating the same injection and kinetic measurement on the next well(s) in the microplate.
Tecan's iControl Software - Kinetic Measurement Basics part 1
Переглядів 6 тис.6 років тому
This video explains how to set up a simple kinetic reading in iControl software. This example is relevant to repeat measurements of the entire plate (aka plate-wise readings). For well-wise kinetics where kinetic events are measured and completed in one well before moving on to another well, look for a Part 2 video on this channel for Well Wise Kinetics.
Tecan iControl Software. How to Create an Absorbance Script
Переглядів 8 тис.6 років тому
This video provides a brief introduction to the creation of an single endpoint absorbance measurement script in Tecan's iControl software. These instructions are relevant to any of the M series Infinite microplate readers. Topics covered include plate definition selection, entry of measurement wavelength, number of flashes, settle time, multiple reads per well, EXCEL data output, and basic scri...
Tecan's Magellan Microplate Reader Software - Creating a Method with Standard Curve Analysis
Переглядів 18 тис.8 років тому
This video describes how to create a simple method file in Tecan's Magellan software that will measure samples and derive concentrations for the samples using a standard curve. The method created here is relevant for use in ELISA assays, Protein & Nucleic Acid quantitation, or other assays where a standard curve is used. This video content does not contain instructions on how to optimize the me...
Tecan's Magellan Microplate Reader Software - Running a Method with Standard Curve Analysis
Переглядів 10 тис.8 років тому
This video describes how to run a simple method file in Tecan's Magellan software that will measure samples and derive concentrations using a standard curve. The method shown here is relevant for use in ELISA assays, Protein & Nucleic Acid quantitation, or other assays where a standard curve is used. This video content does not contain instructions on how to optimize the measurement settings of...
How to measure the Qubit assay in microplates
Переглядів 6 тис.9 років тому
This video shows a simple example of how to adapt the Life Technologies Qubit Broad Range or High Sensitivity dsDNA quantification assay kits to a higher throughput microplate format using Tecan's microplate readers and Magellan software. There are a variety of ways that DNA samples can be prepared and diluted prior to measuring them with these kits. This video example assumes that all samples ...
Installing spark transport pads
Переглядів 4529 років тому
This video shows how to install the pads on the Spark 10M transport so that it can be prepared for shipping. The instrument must be turned off prior to following the video steps.
Tecan Magellan Software - Brief explanation of Kinetic Data Export to EXCEL
Переглядів 7 тис.10 років тому
This video offers a brief explanation of how to export raw kinetic data to Microsoft EXCELfrom a Tecan microplate reader and Magellan software version 7.2.
Tecan Infinite 200Pro - Removing the transport lock
Переглядів 1,3 тис.10 років тому
Video instructions for removing the plate carrier transport lock on a Tecan Infinite 200Pro Microplate reader
Tecan Infinite F200 and F200Pro optical filter management tutorial
Переглядів 8 тис.10 років тому
This video explains how to use the filter holders and remove and install filters on the Tecan Infinite F200 and F200Pro microplate readers. Skip to time @2:00 if you just need to know how to install filters and program the holder.
How to set up a Tecan Infinite 200Pro
Переглядів 4,6 тис.10 років тому
This video overviews the basic setup of a Tecan Infinite 200Pro microplate reader where iControl software has already been installed on the computer prior to connecting the instrument to the PC
Tecan CONNECT Microplate Stacker Overview
Переглядів 2,1 тис.10 років тому
This is a brief video showing the control and operation of the Tecan CONNECT microplate stacker. The video includes examples of how to load plates into the stacks, the timing of plate cycling and control of the instrument with a microplate reader via iControl Software. Note: The stacker has a yellow sticker on it that says " Never use filled plates on this instrument". The CONNECT used in this ...
Tecan Infinite 200Pro installing transport lock
Переглядів 1,1 тис.10 років тому
Tecan Infinite 200Pro installing transport lock
Thanks. Do you know which instrument is best for DNA quantification - Qubit4 or Tecan flurometer? Any publisehd relevant article?
Hey! How can i change the setting to have measurements and shaking after different time intervalls?
amazing
Is there any video for Tecan iControl Software - Flurescence Intensity? Please share. I cannot change Excitation and Emission wavelength. It's locked and two values only. Thanks in advance.
Amazing.
how do you know it take 30s to read the plate?
From experience. The length of read time depends on the number of wells measured, the number for flashes used and whether there is any other settle time entered, or shaking which would result in extra processing time.
I really appreciate your help for these info. Thanks a lot 😊
Pls share video explanation of Fluorescence intensity and fluorescence scan Tq
Good info regarding absorbance Pls upload detailed video regarding fluorescence intensity and fluorescence scan Tq
how can we set up two plates--one is for standard curve and another one is for sample plate?
sorry for not replying sooner as I do not monitor this channel often. For anyone reading the comment who is interested, the software lets you choose how many plates a method will measure. As you begin to enter measurement parameters in Magellan there will be a selection in the lower left of the window to indicate how many plates it should expect. Keep in mind that as you add plates, the software will require that you define the layout for each plate. When creating the layout for the first plate, put your standards on that plate and any other samples or controls that will occupy the plate too. There will be an option along the top of the plate layout window to advance to what the layout will be for the second, third plate etc and you'll want to filly those layouts with the appropriate identifiers. When the method is eventually executed, the software will read the first plate and then prompt the user to insert the next plate, and so on. If the method calls for more plates than you plan to read, that is OK. You will just stop the process of reading once the last plate is measured. The software will then do the analysis on the plates that were measure.
What I'm currently seeing is that "Measurement Wavelength: The filter is not part of the actual filter side" and I have not changed anything.
Hi, I am receiving an error message that "this strip can't be used while the plate is moving out." There are only two absorbance values available now unlike previously that the values can be changed.
Many thanks for this video. Please what can I do if I restarted the software and it is not allowing me to change the absorbance value to the previous value again? It now gives me just two options unlike before that I can change the values.
Hi, somebody can share the iControl software?, I lost the CD and the website only show that I need to buy it aging
Thank you for this detailed video, Regarding the excitation/emission, it’s written 630/680 in the technical sheet of qubit not 490/525 as you showed here. Regards
Hi there is a difference between the DNA and RNA Qubit assays. The HS RNA assay uses 630/680, whereas the DNA kits use 490/525 from what I recall. On page 7 of this manual you can see where they refer to the 630/680 wavelengths for the RNA HS kit. assets.thermofisher.com/TFS-Assets/LSG/manuals/Qubit_RNA_HS_Assay_UG.pdf
I keep coming back to this video time and time again. Thank you so much! Very helpful and informative.
You made my day. :)
Thank you can i read without installing in PC
Only if your plate reader is a Sunrise with a touch screen.
but u dun tell about how to adjust volume of microplate
please i want the software, here is my email aiitech300@gmail.com thanks
Thanks for the information its really helpful I have a question suppose i have generated a kinetic read of 90 mins data wd basic protocol now i want it to export it in a column format( like all A1 wells time points together) not in a plate map manner Can we do it...? Or before read only we have to do setup? I tried but its always gets exported to excel in plate format😊
Sorry for the very delayed response. When editing your method, navigate to the screen where you can edit settings such as Data handling and select “data export”. In the data export window select the “export options” button and pick the export option “result” called Table(data in rows). This will export information for each well across the spreadsheet in rows so that each column will contain a specific type of data you are exporting such as, raw, mean, concentration, etc.
Hi I apologize for never replying to you. I should have turned off comments because I am not monitoring these videos for questions. Either way, here is an answer to anyone else who has a similar question. As you are creating a method or reviewing data that has been collected, there are settings under "Data Export" where the format of the exported data can be driven into matrix, column, or row formats. You will find these in Data Export, "Export Options" visible in the video starting @10:26. To have your data appear with the wells running down the left side of the spreadsheet and columns of data types running across the top, select "Table(well data in rows). Thx
How do you save the data in the excel into pdf ?
In EXCEL, choose save as and select file type "pdf"
ChroMate 4300 PC controlled ELISA microplate reader Measures Absorbance of 96 wells in approximately 12 seconds User-friendly intuitive software Automatically positions plate, blanks, reads and calculates results Uses standard microwell plates for all reactions open system Four filter model (405 nm - 630 nm) Optional 6 filter VIS model (405 nm - 630 nm) I need a definition ChroMate 4300
Hi, I'm trying to run a basic 96-well plate with my samples being 100% solution and a 10x dilution with a row of blanks. The problem is that when I read the plate, my blanks and 10x dilution aren't giving me the correct results. I know what the correct results are because I'm using it in parallel with another spectrometer both at 730 nm. Thanks in advance
Hi! I am using a Tecan Sunrise microplate reader with the Magellan software for doing an standard curve but I have a problem. Whenever I want to run my curve, the machine says that I don't have enough base points... so, what I tried to do was a blank reduction (I am not sure if this was what I supposed to do but I gave it a chance) but I do not have the automatic option in the transformation section to do this. I tried to fix this by putting the formula (x-BL1) directly but it doesn't seem to be working. Can you help me with this please? Thanks!
please go ahead and email me your magellan method directly to rcavicke@roadrunner.com and I will take a look. Because this is not an official Tecan site, I have to take your inquiry to my personal email address. Thanks for understanding.
As long as you have defined at least two wells with the identifier called ST1_1 and ST1_2, and valid data points are collected for these wells, the software can plot a curve. Once you read the plate, if all of the data points for your standards are "OVER" , or if only one standard has a valid data value and the rest are "OVER", and beyond the OD range of the instrument, it will not be able to plot any points you'll get an error that there are not enough base points.
Thank you
Thanks for your presentation, would please show us how to measure luciferase activity? like firefly and Renilla in a Dual-Luciferase Reporter Assay .
love from Bangladesh.. nice video
Thank you for your time explaining so well!