Bitesize Bio
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Episode 76 — Does Your Science Box You In?
#76 - At the heart of the scientific method lies the idea that perspectives are not static-they evolve, shift, and intersect as we tackle scientific problems from different perspectives and uncover new ground truths.
Yet, for most of us, daily science boils down to fine details such as the particulars of experimental design, the caveats to our experiments, producing enough data to satisfy the boss, exporting your data in the correct format, and leaving the instrument ready for the next user.
The list really does go on. And each item is a valid thing to focus on, depending on your perspective.
But how do you maintain clarity amidst these particulars?
If your project involves analyzing a bunch of numbers, how do you avoid the pitfalls of narrow-mindedness and tunnel vision?
And if you were unfettered by so many details and particulars, what would the top research priority be?
In this episode, we look up instead of down and refresh the importance of thinking from different perspectives to avoid tunnel vision and keep your mind open to fresh ideas.
Watch or Listen to all episodes of The Happy Scientist podcast here: thehappyscientist.bitesizebio.com
#Podcast #BitesizeBio #TheHappyScientist
Переглядів: 15

Відео

Contamination and Recovery of PFAS in Analytical Methods Requiring Filter Membranes
Переглядів 814 годин тому
Originally broadcast on 11-Sep-23. Elevate your analysis and liquid chromatography-learn what consumables, materials, and reagents can harm your LC-MS/MS data for more accurate quantification using PFAS as an example. In this webinar, you will: See how contamination from consumables, equipment, reagents, and non-specific binding of samples to syringe filters can make LC-MS/MS quantification ina...
Label-free 3D Live Cell Imaging and Quantification using Holotomography
Переглядів 1016 годин тому
Originally broadcast on 5-Sep-23. Discover how holotomography offers label-free high-resolution imaging of live multicellular specimens in 3D, allowing visualization and quantification of the dynamics and mechanisms of live cells, subcellular organelles, and tissue structures without sample preparation. In this webinar, you will discover: how holotomography provides label-free 4D quantitative i...
Personal Advice on How to Respond to Criticism of Your Grant Proposal Reviews
Переглядів 1319 годин тому
#113 - Learning how to respond to criticism is an unavoidable aspect of grant applications. Follow all instructions from funding agencies to avoid immediate rejection. This episode of Mentors At Your Benchside gives you some simple strategies to ensure you respond to criticism in a constructive and proportionate way. For example, identifying reviewers can help you tailor your responses, but don...
Sample Filtration in Pharmaceutical QC Testing
Переглядів 719 годин тому
Originally broadcast on 27-Jul-23. Maximize the reliability and accuracy of your in vitro dissolution testing by selecting the right filtration devices, optimizing throughput, and improving test accuracy and reproducibility. In this webinar, you will learn: • The importance of filtration in dissolution testing. • How proper selection of device formats can improve throughput. • The effect of mem...
Eppendorf Webinar 28 Sep 23 OD Automate Your Pipetting - Unleash Your Potential
Переглядів 1321 годину тому
Originally broadcast on 28-Sep-23. Explore cutting-edge, budget-friendly automated liquid handling systems designed to eliminate errors, improve pipetting consistency, and simplify routine workflows. In this webinar, you will learn: - The benefits of automation. - When to consider automating liquid handling workflows. - How automation can support even low-throughput applications. Despite the gr...
Ten common statistical mistakes to watch out for when writing or reviewing a manuscript
Переглядів 1121 день тому
Originally broadcast 15-Sep-23. Discover the most common statistical and reporting errors in the scientific literature and learn reliable methods to increase the rigor of your data and its reporting. In this webinar, you will: - Discover the benefits of transparent reporting; - Explore common statistical mistakes and the solutions to them; - Gain the tools to assess the reliability of your find...
How to Keep Your ULT Freezer Healthy
Переглядів 9Місяць тому
Originally broadcast on 7-Sep-23 Do you ever consider the value of the samples within your freezer? Your ultra-low temperature (ULT) freezer is not just a storage room for samples; it is your assurance for long-term scientific success and guards the results of your work. Therefore, every researcher should understand the importance of ULT freezers, know how to choose a reliable one, understand b...
Episode 75 - Patience and Getting What You Want. Are They Compatible?
Переглядів 17Місяць тому
#75 - We're told from a young age that patience is a virtue. But does that hold up to scrutiny? In some respects, it seems so, given that impatience can be annoying to experience and make you unbearable to the people you're waiting on. But what are the consequences of excessive patience? Does it become a pathological trait that results in excess tolerance and forbearance, which, in turn, stop y...
Errors and Misconduct in Biomedical Research
Переглядів 38Місяць тому
Originally broadcast on 4-Aug-24 Embark on an eye-opening journey with image forensics detective Elisabeth Bik as she uncovers the startling prevalence of duplicated images and research misconduct in scientific papers. Discover what drives scientists to research misconduct and learn how to report and combat these issues within journals and institutions. In this webinar, you will learn: - Why so...
Garbage In, Garbage Out: Sample Prep for Flow Cytometry
Переглядів 42Місяць тому
Originally broadcast on 11-Aug-23 Learn how to optimize your flow cytometry protocol from creating viable single-cell suspensions to staining, fixation, and improving data quality. In this webinar, you will learn: • Strategies to prepare samples optimized for your experiments; • Factors to consider when designing a new experiment; • How to improve your data. The quality of flow cytometry data d...
Benefits of Imaging Data in Flow Cytometry Experiments: A Panel Discussion
Переглядів 242 місяці тому
Originally broadcast on Thursday, June 29th, 2023. Experience the transformative power of unbiased, data-driven cell analysis with access to over 25 image-derived label-free parameters, empowering you to easily assess sample quality, optimize gating strategies, and validate rare events. In this webinar, you will learn how automated image analysis combined with flow cytometry can help: • Improve...
Isoelectric Focusing: A Simple Way to Enhance Your Protein Separation
Переглядів 182 місяці тому
#112 - Isoelectric focusing (IEF) is a powerful technique distinct from the more familiar SDS-PAGE, [1 ( bitesizebio.com/580/how-sds-page-works/ ),2] ( bitesizebio.com/26811/isoelectric-focusing-separation-proteins-peptides/ ) tailored for separating proteins or peptides based on their isoelectric points (pI). [3] ( bitesizebio.com/9425/what-is-ph-pka-pi/ ) This method capitalizes on the migrat...
Episode 74 - What Makes it Onto Your To-do List and Why?
Переглядів 152 місяці тому
Episode 74 - What Makes it Onto Your To-do List and Why?
Top 10 Uses of Microbes in Biotechnology
Переглядів 992 місяці тому
Top 10 Uses of Microbes in Biotechnology
Let’s Talk About Stats: Methods for Comparing Two Sets of Data
Переглядів 133 місяці тому
Let’s Talk About Stats: Methods for Comparing Two Sets of Data
Episode 73 - What Gets You up in the Morning to Do Science? Why Knowing The Reason Matters
Переглядів 113 місяці тому
Episode 73 - What Gets You up in the Morning to Do Science? Why Knowing The Reason Matters
Flow Stars Recorded Live at CYTO 2024
Переглядів 583 місяці тому
Flow Stars Recorded Live at CYTO 2024
Personal Advice on Building Your Professional Network. It Takes a Village
Переглядів 193 місяці тому
Personal Advice on Building Your Professional Network. It Takes a Village
Precision and Efficiency at Your Fingertips: Why Electronic Pipettes are the Future of Pipetting
Переглядів 103 місяці тому
Precision and Efficiency at Your Fingertips: Why Electronic Pipettes are the Future of Pipetting
4 Fixatives for Histology and Cytometry: Perfect Your Preservation
Переглядів 233 місяці тому
4 Fixatives for Histology and Cytometry: Perfect Your Preservation
Everyday Culture Practice Improving Reproducibility in Cell Culture
Переглядів 193 місяці тому
Everyday Culture Practice Improving Reproducibility in Cell Culture
Introduction to Liquid Handling for NGS Workflows
Переглядів 354 місяці тому
Introduction to Liquid Handling for NGS Workflows
Real-time IntraVital Microscopy: In Vivo Cellular-level Imaging of Internal Organs in a Live Animal
Переглядів 374 місяці тому
Real-time IntraVital Microscopy: In Vivo Cellular-level Imaging of Internal Organs in a Live Animal
Episode 72 - Why Keeping Your Options Open Is Holding You Back
Переглядів 144 місяці тому
Episode 72 - Why Keeping Your Options Open Is Holding You Back
Microscope Disinfection: A Quick Guide
Переглядів 124 місяці тому
Microscope Disinfection: A Quick Guide
Essentials in Centrifugation - Better Safe than Sorry!
Переглядів 214 місяці тому
Essentials in Centrifugation - Better Safe than Sorry!
Reproducibility: Mastering the Art of Pipetting
Переглядів 294 місяці тому
Reproducibility: Mastering the Art of Pipetting
Tissue Processing For Histology: What Exactly Happens?
Переглядів 484 місяці тому
Tissue Processing For Histology: What Exactly Happens?
The Good, the Bad, and the RNA: Hunting for Molecular Similarities in Interstitial Lung Diseases
Переглядів 1164 місяці тому
The Good, the Bad, and the RNA: Hunting for Molecular Similarities in Interstitial Lung Diseases

КОМЕНТАРІ

  • @FreelancerSabina42
    @FreelancerSabina42 3 дні тому

    I saw your UA-cam channel. Your content and videos are good. Hope it will be monetized very soon.

  • @FreelancerSabina42
    @FreelancerSabina42 3 дні тому

    I have subscribed to your UA-cam channel, your channel is very good. 😊❤

  • @aaryanpatel7229
    @aaryanpatel7229 4 дні тому

    I was expecting some animation or atleast board work to explain this topic.

  • @fl_snorkeldork
    @fl_snorkeldork 8 днів тому

    HEK-293 came from a baby named Johanna, she was 3 months gestation when her organs were harvested. Her name qas Johanna. Leta say her name. The 293 refers ro the number of experiments it took to get the cell line. 293 babies before Johanna also died...with no pain meds either.

  • @demo-hh
    @demo-hh 10 днів тому

    good

  • @NairlakshmiSurendran
    @NairlakshmiSurendran 18 днів тому

    Hai can you help me with flow cytometry calculations ? How to Find initial dilution factor ??

  • @danshabash
    @danshabash Місяць тому

    Worth noting that those 40uM filters that go on 5ml FACS tubes are several times cheaper if you buy them separately, rather than with a tube. Saves a lot of money

  • @FelicitasFree-y9h
    @FelicitasFree-y9h Місяць тому

    Crona Course

  • @preciouspreyeayawei9626
    @preciouspreyeayawei9626 4 місяці тому

    This is a very wholesome conversation on gene editing. Thank you.

  • @amelbelanes4684
    @amelbelanes4684 4 місяці тому

    ❤❤❤❤

  • @amelbelanes4684
    @amelbelanes4684 4 місяці тому

    I enjoyed it 🎉🎉🎉🎉 Thank you very much ❤❤❤

    • @BitesizeBio
      @BitesizeBio 4 місяці тому

      You are very welcome! Thank you.

  • @HEKProductions
    @HEKProductions 6 місяців тому

    wait what the HEK??????? thats me! HEK i'm a cell....

  • @nareshbarik5384
    @nareshbarik5384 7 місяців тому

    Thank you ❤

  • @lukaschumchal6676
    @lukaschumchal6676 8 місяців тому

    Thank you for nice summary :)

    • @BitesizeBio
      @BitesizeBio 8 місяців тому

      You're welcome. Thank you for the feedback. 👍

  • @SciFrank38
    @SciFrank38 9 місяців тому

    It is been a while since I used flow cytometry, I miss it, so cool video :D thank you very much for the info an bringing up the memories :D

  • @SciFrank38
    @SciFrank38 9 місяців тому

    Thank you for the info

  • @amnasarwar39
    @amnasarwar39 9 місяців тому

    how -20 C is achieved ?

    • @BitesizeBio
      @BitesizeBio 9 місяців тому

      Hi. Here are the common methods. Freezers and Refrigerators: Laboratories often have freezers that can maintain temperatures as low as -20°C or even lower (like -80°C). These freezers are used to store biological samples, reagents, and to conduct experiments that require low temperatures. Ice Baths with Salt: For quick cooling or maintaining low temperatures for a short duration, ice baths can be used. Adding salt to the ice water can lower its freezing point, allowing temperatures to reach approximately -20°C. This method is often used for short-term applications.

  • @APC-pm2on
    @APC-pm2on Рік тому

    nice presentation. thanks

  • @Wyllyum
    @Wyllyum Рік тому

    I knew I liked Gary Nolan, but when he said he played EverQuest…I fell in love. ❤

  • @pamelameeks3772
    @pamelameeks3772 Рік тому

    I can't believe you don't have more views. Professor Nolan videos are almost non existent. . He is so fascinating, and yet down to earth unlike so many highly intelligent people. Thank you so much, and to Professor Nolan as well.

  • @kathymravintz9218
    @kathymravintz9218 2 роки тому

    Loved this interview. Great to get to know the professor as a person.

  • @tobyearl7433
    @tobyearl7433 2 роки тому

    Thanks for a nicely put interview with the much appreciated Mr Nolan. Interesting with quite a different perspective with reference to the podcasts by Lex Fridman and Jesse Michaels (American Alchemy), which I do recommend to consider for further investigation ... 🖖💚

  • @SimpleLifeAlways81
    @SimpleLifeAlways81 2 роки тому

    I love this interview … the random questions and the smiles … it was a sweet and awesome interview.

  • @dr.ibneyali6070
    @dr.ibneyali6070 2 роки тому

    Wow madam great knowledge about PCR

  • @zakkamarachi8081
    @zakkamarachi8081 2 роки тому

    Wow i want to become like him.

  • @francinefinlay8810
    @francinefinlay8810 2 роки тому

    Hey Dr Garey Nolan u want to know how serious I am I want a job and I want a sign up bonus my job real start two months after my sign up bonus Ian a alien hunter alien blood runs threw my veins they aren't brothers after 4years of fighting and learning and teaching them we became brother you that alien man on the table the one you are showing he is real and the USAF was chasing a star well a few of them there is probably a 100 or more different types of aliens the star aliens are one and my job teaching people about aliens which you can have a job at NASA still come to my class room which will be a big ass motor home that you will get together and buy me with my sign up bonus I think Ian worth way to much but I will be cool and work out a deal ok just with my power I made the alien come out of the dead alien eye the right one if u need proof got the two pictures and ready to send Dr Garey Nolan we will be good friends my wife is all most behind me see seen the space ship sent to Twitter was gone the next day

  • @5lanediver
    @5lanediver 2 роки тому

    excellent interview. i subbed. thanks for putting this out there, and taking the time! i am glad that gary entertained your more lighthearted questions - i was worried he would think it was a waste of time!

    • @SimpleLifeAlways81
      @SimpleLifeAlways81 2 роки тому

      No, it’s never a waste of time to get to really know a person who is important to the interviewer. Little details would help the interviewer know what to ask, how to ask, and so on.

    • @5lanediver
      @5lanediver 2 роки тому

      @@SimpleLifeAlways81 i didn’t say it was a waste of time. i said i was glad gary didn’t see it as a waste of time. some more serious people might think that, i am glad he did not.

  • @Brog9
    @Brog9 2 роки тому

    Fascinating interview

    • @BitesizeBio
      @BitesizeBio 2 роки тому

      Thanks! We couldn't agree more and we're glad you enjoyed it as much as we did!

    • @Sapientiaa
      @Sapientiaa 2 роки тому

      @@BitesizeBio Please put time stamps on your videos.

  • @andreabrook7581
    @andreabrook7581 7 років тому

    Immunohistochemical staining: DAB color. www.creative-bioarray.com/Services/Immunohistochemistry-IHC.htm

  • @andreabrook7581
    @andreabrook7581 7 років тому

    Some cells were used as antigens, and the corresponding antibodies were prepared. Immunohistochemical staining was performed to determine the origin of the cells. www.creative-bioarray.com/Services/Immunohistochemistry-IHC.htm