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ClevaLab
Australia
Приєднався 12 гру 2020
ClevaLab makes easy-to-understand animated biology videos. These explain the basic principles of human biology, including cell biology and molecular biology. Subscribe to be notified of a new video, follow our Instagram or TikTok, or join the email list on our website. Come learn biology with us!
I'm Nicole Jones, and I create ClevaLab, including images, video content, animation and audio. I have a Biomedical Science Degree and a PhD in Pathology. I've spent six years working as a Post-Doctoral Scientist in the lab. During my Post-Docs, I studied Inflammation, Immunology, and Cancer. I then moved into the Biotechnology industry. In Biotech, I've worked as an Application Scientist for the past ten years. Teaching students and Scientists a wide range of Molecular Biology techniques. From extraction of DNA and RNA to Next Generation Sequencing.
I'm Nicole Jones, and I create ClevaLab, including images, video content, animation and audio. I have a Biomedical Science Degree and a PhD in Pathology. I've spent six years working as a Post-Doctoral Scientist in the lab. During my Post-Docs, I studied Inflammation, Immunology, and Cancer. I then moved into the Biotechnology industry. In Biotech, I've worked as an Application Scientist for the past ten years. Teaching students and Scientists a wide range of Molecular Biology techniques. From extraction of DNA and RNA to Next Generation Sequencing.
Sanger DNA Sequencing, From Then to Now.
This video explores the basics of Sanger sequencing and the fascinating history behind this groundbreaking technology. It explains the basics of DNA sequencing, including the components necessary for the reaction and the steps involved. Topics also include ddNTPs, cycle sequencing, fluorescent dyes and capillary electrophoresis.
We'll also compare Sanger sequencing to other sequencing technologies, such as NGS, to give you a better understanding of how it all works. Throughout the video, we'll take a chronological approach to explore the history of Sanger sequencing, starting with its development in the late 1970s and following its evolution. By the end of the video, you'll deeply understand Sanger sequencing and its role in sequencing the human genome.
So grab a cup of coffee and journey through the fascinating world of Sanger sequencing! Whether you're a student, researcher, or just curious about the science behind DNA sequencing, this video is for you!
🌏 www.clevalab.com
📖 This video is also a blog post with images, references and a PDF download; visit: www.clevalab.com/post/sanger-dna-sequencing
👉 You may also like: "Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing." ua-cam.com/video/WKAUtJQ69n8/v-deo.html
Visit the ClevaLab Channel Page for more videos: www.youtube.com/@ClevaLab
✉️ Sign up to the email list to be notified of new videos: www.clevalab.com/email-subscription
SOCIAL MEDIA LINKS
Twitter: _ClevaLab
Instagram: _clevalab
Pinterest: www.pinterest.com.au/ClevaLab
Tiktok: www.tiktok.com/@clevalab
Facebook: ClevaLab
CHAPTERS:
00:00 The 1977 Invention of Sanger Sequencing
00:23 The Basics of How DNA Is Copied
00:36 What are dNTPs & ddNTPs anyway?
01:19 How Does a ddNTP Stop DNA Copying?
01:54 The DNA Naming Convention 5' to 3'
02:43 Which Bases Go Together in Base Pairing?
03:05 Steps of the First Sanger Sequencing Method
06:11 The First DNA Sequencing Instrument, the AB370A.
07:20 The Launch of the Human Genome Project
07:46 How Does Cycle Sequencing Improve Things?
08:59 Automated DNA Separation by Capillary Electrophoresis
09:36 ABI PRISM 310, Modern Sanger Sequencing
10:49 Fluorescent ddNTPs and the Invention of BigDyes
11:33 The Instrument That Sequenced the Genome
13:09 Why Use Sanger Sequencing When There's NGS?
#ClevaLab #sequencing #sangersequencing
We'll also compare Sanger sequencing to other sequencing technologies, such as NGS, to give you a better understanding of how it all works. Throughout the video, we'll take a chronological approach to explore the history of Sanger sequencing, starting with its development in the late 1970s and following its evolution. By the end of the video, you'll deeply understand Sanger sequencing and its role in sequencing the human genome.
So grab a cup of coffee and journey through the fascinating world of Sanger sequencing! Whether you're a student, researcher, or just curious about the science behind DNA sequencing, this video is for you!
🌏 www.clevalab.com
📖 This video is also a blog post with images, references and a PDF download; visit: www.clevalab.com/post/sanger-dna-sequencing
👉 You may also like: "Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing." ua-cam.com/video/WKAUtJQ69n8/v-deo.html
Visit the ClevaLab Channel Page for more videos: www.youtube.com/@ClevaLab
✉️ Sign up to the email list to be notified of new videos: www.clevalab.com/email-subscription
SOCIAL MEDIA LINKS
Twitter: _ClevaLab
Instagram: _clevalab
Pinterest: www.pinterest.com.au/ClevaLab
Tiktok: www.tiktok.com/@clevalab
Facebook: ClevaLab
CHAPTERS:
00:00 The 1977 Invention of Sanger Sequencing
00:23 The Basics of How DNA Is Copied
00:36 What are dNTPs & ddNTPs anyway?
01:19 How Does a ddNTP Stop DNA Copying?
01:54 The DNA Naming Convention 5' to 3'
02:43 Which Bases Go Together in Base Pairing?
03:05 Steps of the First Sanger Sequencing Method
06:11 The First DNA Sequencing Instrument, the AB370A.
07:20 The Launch of the Human Genome Project
07:46 How Does Cycle Sequencing Improve Things?
08:59 Automated DNA Separation by Capillary Electrophoresis
09:36 ABI PRISM 310, Modern Sanger Sequencing
10:49 Fluorescent ddNTPs and the Invention of BigDyes
11:33 The Instrument That Sequenced the Genome
13:09 Why Use Sanger Sequencing When There's NGS?
#ClevaLab #sequencing #sangersequencing
Переглядів: 43 892
Відео
Next Generation Sequencing - A Step-By-Step Guide to DNA Sequencing.
Переглядів 281 тис.Рік тому
Next Generation Sequencing (NGS) is used to sequence both DNA and RNA. Billions of DNA strands get sequenced simultaneously using NGS. Whereas with Sanger Sequencing, only one strand is sequenced at a time. While the Human Genome Project took over 30 years to sequence the human genome for the first time. Now with Next Generation Sequencing, a whole human genome can be sequenced in just one day....
What are Autoimmune Diseases and How Do They Develop?
Переглядів 168 тис.2 роки тому
What are Autoimmune Diseases? Autoimmune Diseases are caused when the immune system attacks its own cells. This video is about autoimmunity, how autoimmune diseases develop, and the symptoms they cause. Find out how autoimmune antibodies, B-cells and T-cells get made and how they get activated. Autoimmune cells are activated via molecular mimicry, bystander activation and epitope spreading. Per...
Mast Cells | Normal Role, Allergies, Anaphylaxis, MCAS & Mastocytosis.
Переглядів 60 тис.2 роки тому
Find out all about mast cells, their usual role in fighting infections and how they can cause allergies and anaphylaxis when things go wrong. Also, find out about the mast cell diseases MCAS and mastocytosis. Mast Cells line the tissues of the body exposed to the outside world. They protect us from foreign invaders, including bacteria, viruses, parasites, and venoms. When foreign invaders are d...
What is PCR and qPCR? | PCR Animation
Переглядів 162 тис.2 роки тому
Discover molecular biology and the Polymerase Chain Reaction (PCR). What is PCR and qPCR? PCR takes DNA, nucleotides, primers, and Taq Polymerase to make unlimited DNA. This target DNA gets seen by running it on an agarose gel in PCR or detecting it with a camera in qPCR. With qPCR probes labelled with different colours can amplify many targets per tube. PCR and qPCR get used in disease diagnos...
Growing Organs | Stem cells, Organoids and 3D Bioprinting.
Переглядів 28 тис.3 роки тому
Growing organs in the Lab - Find out how scientists are making human organs in the lab from stem cells. While we can't grow fully functional human organs yet, they can grow organoids from stem cells to study organ development and 3D bioprint tissues that can one day be used to repair organs. 🌏 www.clevalab.com 👉 You may also like: The Basic Principles of a Cell, ua-cam.com/video/R5z0VYBnZPs/v-d...
Understanding Cell Functions in the Human Body.
Переглядів 21 тис.3 роки тому
This video will give you an understanding of cell functions in the human body. The animation will guide you through the human body's basic cell structure and function. Also, discover how cells form tissues and organs. as well as how proteins get made from DNA. The types of proteins produced by a cell determine its function and structure. Learn about prokaryotic and eukaryotic cells and how plan...
Useful topic, Tank you
I'm glad it helped. 🤓 Thanks for your comment.
great videos and channel
Thank you! 🤓
might be the first time i hear it referred to as the oxygen, instead of the hydroxide
the thing is i hate any topic that has to do with NGS, it makes me hate biology or biochemistry but anyways thanks...is for marks and getting done with it
thanks
You're welcome! 🤓
Amazing video. It helped me a lot!
Glad you liked it! 🤓
Im scared im gonna get an autoimmune decease with the path im on
I searched for this video beacouse, when i was watching dr House, the topic of autoimmune disease was brought UP a lot but even tho im allready on season five it still isn't explained and im strugguling for knowledge, So thank you for fulfiling my hunger for information xD
Great, I'm glad it helped you. 🤓
Great video thank you very much
You are very welcome, I'm glad you enjoyed it. 🤓
PLEASE HOW THE DNA GOT SINGKE STRANDED ??!
Thanks for the information............
Thanks for your comment. 🤓
I have a question about illumina sequencing. ASAP let me know plz..! I’m high school student.. How is DNA synthesized in the 3 to 5 direction in the reverse strand? Please let me know about reverse strand!!
Very clear. Thanks.
I'm glad you liked it. Thanks for your comment. 🤓
First time I've actually understood this, thank you!!🤩
Great, I'm so glad it helped you. 🤓 Thanks for your comment.
Amazing video, I got to know about many things yhrough this. I am currently trying to get to know about how to write research paper and for that I chose a topic of next generation sequencing can you provide me a pdf for that purpose?
where are the ankylosing spondylitis baddiezzzz
This is complicated to understand even with visual help, but in real life when you can't see any of this happening, it's basically just magic. I don't understand any of it
mnra creates it,
Is there any relation for mouthful ulcers and this autoimmune disease plz explain
Thank you, how does the index help identify the sample? I always wonder how do you know how to put it all back in order? Thank you so much!!
"17 per cent" is by far no fact and a theory with no data basis. The prevalance based of the ICD 10 codes is about 6 per cent per 100.000. J Hamilton once said numbers of 1 per cent.
Can't thank you enough!!!❤
You're welcome. 🤓
i don't understand why there is only one primer in Sanger sequencing TT
Somehow after a week of my professor trying to explain this to us and failing, this video helped me understand it in 7 minutes.
It's great to hear it helped you. 🤓 Thanks for your comment.
Best and detailed video
Thanks for your comment. 🤓 I'm glad you liked it.
Bravo.....ok......estract dna from urea.....cheers
Thanks for your comment. 🤓 Urea is a compound (a pure substance made of two or more elements chemically bound together). Do you mean urine? You can extract DNA from urine. There's protocols for this using commercial DNA extraction kits.
@@ClevaLab urine.....yes.......stem cells how to........
4:25 FINALLY I GOT IT!! Everyone explains the detection like the fluorescence from the previous nucleotide just disappears. Thank youuuuuuuuuuuuuuuuuuuuuuu for eternityyyyyyy
So great! It makes a difference when you understand each step. 🙌 Thanks for your comment. 🤓
Thank you for everything
You're welcome. 🤓 Thanks for your comment.
Your videos are amazing. They are clear, easy to understand, rich in contents, well illustrated and short. Perfect! Only a correction, the GHP complete 92% of the human genome instead of 85%.
I have to say the video explains things better than my professor...😅
I'm glad it helped you. 🤓 Thanks for your comment. Maybe your professor should show the video in class!
This is AMAZING!!!!!!! Helped summarize (in detail) a 50 minute lecture in just 7 minutes!
Thanks for your comment. 🤓 That's great to hear, I'm glad it helped you.
Intestinal, curious as to why...
This is beautiful
Thanks for your comment. 🤓 I'm glad you liked it.
why reverse strand is washed off ??? @ClevaLab
Lot of progress since I took biochemistry in 1970.
Yes, there has been! Thanks for your comment. 🤓
Thank you so much for this video! I have an autoimmune disease and this video helps me understand what’s going on.
Thanks for your comment. 🤓
Hello, thanks for the explanation it was very very clear. Thank you also for a previous response explaining why we can be certain to get every nucleotide sequenced during the first iteration. But i just had one question about the comparison between Sanger sequencing and NGS, when you talked about sensitivity to detect a base within a background of other DNA, i didn't understand if it was a bad thing or not. Only* 15-20% implied that greater should be better or am i incorrect ?
Thank you, this was very informative.
Thanks for your comment. 🤓 I'm glad you liked it.
great vid love u <3
Thanks for your comment. 🤓 I'm glad you like it.
Am I right that since the moment of filtering and maping it is all done with software, not sequencer?
Thanks for your comment. 🤓 That's correct. Once the sequence is read by on the sequencer, the filtering, mapping, and further analysis are done with software on a computer.
If the definition of immune is to be "totally or partially resistant to a particular infectious disease or pathogen" Auto-immune disease seems like a misleading name, why not call it an immune system disorder or ISD?
I am dealing with 2 Autoimmun Disase one is Hashimoto Disase the other is a Rare Autoimmun Disase Morbus Behcet i feel sick all the time not easy
I'm sorry to hear that. It definitely is not easy. 😔
The Particularity of Neo-Natal Cardiac Pump Susceptibility, of Neo-Natal Auto-Immunity, Is Effective By Embryonic Central Nervous System Development: From the Moment of Birth, the Necessary Search Should Be Which Auto-Immune Virus Is Culpable, to Further and Advance Comprehend Why and How of Patho-Genesis: Auto-Immunity Is Not By "Mistakes", Patho-Genesis Evolves to Organ Failures: All Auto-Immune Dis-Eases Are Human Immunology Viruses, As Acquired Immunology Deficiency Syndromes: One very Actual Factor, of Auto-Immunity Dis-Eases, Is Host-Only Patho-Genetic Expressions, As "Distortions", Rarely As "In-Flammation", Commonly Known As the Concept "In-Flammation", But, By the Terminology "Infection": Meaning, Host-Transmission Is Possible, But, [Usually] only Expressive By Recipient/ Host Infective In-Flammation [Signs and Symptoms]: [Neo-Natal Auto-Immunity] "Neonatal autoimmune diseases are distinctly rare. Most neonatal autoimmune diseases result from the transplacental transfer of maternal antibodies directed against fetal or neonatal antigens in various tissues. In neonatal lupus, the heart seems to be particularly susceptible. Mar 7, 2012": "A healthy immune system defends the body against disease and infection. But if the immune system malfunctions, it mistakenly attacks healthy cells, tissues, and organs. Called autoimmune disease, these attacks can affect any part of the body, weakening bodily function and even turning life-threatening." 😶. Respectively. Tanika Nakeya Lewis, 6127
Thanks ClevaLab! I'm an high school student. And I'm supposed to write an essay about NGS for the school club. Thanks to these explanations and animations I could understand how it works. Cheers!
That's great to hear, I'm so glad. 🤓 Thanks for taking the time to comment.
Great vedio ,it just reminds me of kinda animation of @amebasisters .
Thanks for your comment. 🤓 I choose to take that as a compliment.!
@@ClevaLab 🙂😂
Coud you do an explanation for elementary/middle school students?
Thanks for your comment. 🤓 I'm currently aiming for university level and above, but it's a good idea. I'll put it on my list. 👍
@@ClevaLab I understand! Thanks!
Thanks. The Human reference DNA how was create?
Thanks for your comment. 🤓 The human reference genome was created by Sanger sequencing done by the Human Genome Project. I also have a video on Sanger sequencing here: ua-cam.com/video/X9566yI2cBo/v-deo.html
@@ClevaLab thanks a lot :)
Where was this video all my life!! Excellent explanation of the concept.
I'm so glad it was helpful. 🤓 Thanks for your comment.
Thank you so much, you can't imagine how much you have helped me. I have been confused for more an year and ths cleared everything
Thanks for your comment. 🤓 I'm so glad it was helpful.
Thank you so much, you can't imagine how much you have helped me. I have been confused for more an year and ths cleared everything