Lecture 05: Making Tris Buffer (pH = 8.2)

[@ratnarabha3386]

I m very much thankful for the video..

[@hollyking9208]

Around the 29 minute mark. He says to measure out 3 grams of what? why only 3 grams?

[@hefilial]

But how are you sure that after adding the water to the solution to make it to the desired volume, the pH is the same?

[@NOIXize]

In theory the pH shouldn't change since the water should be neutral :>

[@hefilial]

@@NOIXize but the volume has now increased 🤔

[@hefilial]

So the concentration of H+ ions should decrease now

[@NOIXize]

@@hefilial but the concentration of OH- also decreases :). However they stay balances in the same proportions

[@hefilial]

@@NOIXize so basically you are saying if I take a concentrated acid and add water to it the pH won't change🙄. This doesn't make sense. Increasing volume decreases concentration therefore pH which is defined as negative log of H+ ion concentration also increases.(upto a limit)

[@munmunkundu9033]

How long I can store Tris buffer

[@gurralamohankumar5838]

Thank u

[@smegnewmelese5212]

hi guys? what is difference between tris buffer and tris base?

[@progamingnl776]

If I remember correctly, tris base is used to make a tris buffer

[@padmamanandhar7438]

Very long and elaborative, you could have not included so much things