🔬 How are microscope objectives different? | Amateur Microscopy
Вставка
- Опубліковано 27 чер 2024
- Here I will explain the differences between Achromatic, Fluorite, Apochromatic (APO) and Super Apochromatic (SAPO) microscope objectives. I will also explain chromatic and spherical aberration and field curvature.
🎈 SUPPORT THE CHANNEL
Become a Patron: / microbehunter
🔬 MICROSCOPE RECOMMENDATION (links to the Shop below each video)
Children’s/Introductory - Swift or National Optical SS110: • 🔬 Good and inexpensive...
For Amateurs - Swift SW380T (with phototube) or Swift SW380B: • 🔬 Pretty good microsco...
OM36 Microscope: www.microscope.com/compound-m...
👜 AMAZON AFFILIATE SHOP - As an Amazon Associate I earn from qualifying purchases.
US/World: www.amazon.com/shop/microbehu...
Canada: www.amazon.ca/shop/microbehunter
Germany/Europe: www.amazon.de/shop/microbehunter
UK/Europe: www.amazon.co.uk/shop/microbe...
🎬 UA-cam CHANNELS
Microbehunter: / microbehunter
Microscopic Mysteries - Guess the specimen: / @microscopicmysteries
Microbe Talk - Ask a microbiologist! / @allthingsmicrobes
💻 OTHER LINKS
Main Website - Tons of microscopy info: www.microbehunter.com
MicroWorldArchive - www.microworldarchive.org
Forum - Connect to other microscopists: www.microscopy-forum.com
Instagram - Cool pictures I made: / microbehunter
Facebook - Here I post videos: / microbehunter
✔ Check these Hashtags! #microbehunter #science #microscope - Наука та технологія
thank you!!! all superclear now
Great information top post
Per Leica's description: - quote - Leica’s PL S-APO objectives come very close to the top-quality objectives of the Leica PL APO class - end quote -, therefore s-apo stands for semi-apo which puts them in between the fluotars and the apo's. The IR corrected apo's are named IRAPO by Leica
Now it's clear to me why the price is so different
Thanks a lot
Very informative video, thanks!
An excellent book for anyone who is into the micro world is
FUNDAMENTALS OF LIGHT MICROSCOPY AND ELECTRONIC IMAGING
by Douglas B. Murphy
Awesome video thank you!
I would just like to present some info for your viewers so that they can appreciate the level of cost for routine Olympus microscopes and
objectives for medical pathology labs. Olympus is the leader in North America for pathology. Going back several years, the U Plan SAPO
series of objectives were commonplace among active, working, pathologists who use BX50, 60 series microscopes in their labs (and offices).
For example, a basic Olympus U Plan SAPO 20x objective (P/N 1-U2B825) would set you back $2948 new (2019 pricebook). Pathologists
use 2x, 4x, 10x, 20x, 40x APO's (or SAPO's) most of the time. The need for 60x and 100x is more important for hematopathology in special cases.
And, ........and, ...this is only for "brightfield" inspection and analysis of tissue specimens to arrive at diagnostic details and outcome(s).
Much thanks once again to Oliver for his time, talent, knowledge, and GREAT presentations !
so the Journey To The Microcosmos youtube channel is using Super Apochromatic Objectives because of the pretty darn good image quality?
They are using Differential Interference Contrast (DIC), which gives a 3D like appearance. This has nothing to do directly with the type of objective used, but I assume that they have at least Fluorite objectives, because this is what is common when using this type of technique.
Very interesting information thanks
Great video!
Sir can u make video on stentor corelus and how to culture them and where to find them
You are the geeky microscopy god i have been looking for ^^
Hi, great detail video, thanks. I am interested in photography only, What objective do you use for photography? Looking to invest in a setup but 2nd hand.
Hi Oliver, how can I make it more comfortable to look through the eyepiece because sometimes its uncomfortable can you please tell me how. thankyou if you could.
Located in Canada here, I have been having a hard time figuring out what would be the next step up from the readily available SWIFT SW380T and where I could find other options?
Hello Oliver. Can you use a trinocular/binocular compound microscope as a metallurgical microscope if you stick a flashlight into one of the eyepieces? I'm interested in looking at silicon dies at high magnification, hoping to see the Bayer filter on an old phone/webcam VGA camera sensor, etc. Thanks.
Tried it once. Internal reflections reduced the image quality a lot.
Hi I have a microscope with exchangeable eyepieces and I want to know how to store them without damaging or dirtying them.
What do you use do you already have a video on your microscopes
This was helpful. I am looking to upgrade my Brunel SP35 binocular microscope to switch from the supplied objectives to Nikon ones. But looking at the various options, I am not sure buying Nikon Achromatic objectives at nearing £442 for a 10x, 40x and 60x inc £12 postage from a reputable supplier makes any sense or would be noticeably sharper than the ones I have. I adore Nikon lenses as I have been using Nikon lenses in photography for almost 50 years now and know how good they are, but is spending so much for the same type of lenses worth it just for the sake of having a Nikon brand?
The Nikon Plan Apochromatic lens, I would love to have a 20x one, is away beyond my budget and only by some miracle would I ever be able to afford one.
Hi I'm new to microscope but interested in viewing blood cells any microscope with camera best for blood cells under $1000?
Sir, I'm a student interested in buying a microscope, do you have any recommendations for student microscopes also, could you also please make a video about infinity objectives and what are your thoughts about them.
Thanks, sir and love from India!
ua-cam.com/video/fAeE6ZyfL0k/v-deo.html
No specific recommendations as the requirements and finances are so different from person to person. For kids maybe the Swift SS110 and for more advances the Swift SW380T. I made reviews about both.
@@MicrobehunterMicroscopy Thanks a lot sir!
Hi Oliver; good info on the objectives; would you speak about changing a microscopes magnification by changing the eye piece / oculars. 'Standard' is usually 10x; but what about changing to 5x, 20x, 25x, 30x... ; for both stereo and compound scopes? I have a small stereo scope, that came with 10x for 20x and 40x magnification; for that mag', I found the field of view to be too small, so I changed to 5x eye pieces for 10x, 20x magnification; larger field of view; much better for my general purpose... Thanx...
Lower magnification eyepieces (5x instead of 10x) is possible, but higher magnification will produce larger and more blurry images. So if you want higher magnifications, then you should change objectives to get a clear picture. High mag eyepieces also might be less comfortable to use, due to lower eye-relief (have to move closer to eyepiece to see)
yes; there are 'trade offs' and 'compromises'; striving towards 'perfection' costs money :-) :-) ... thanx for the reply; thanx for the videos!!!... @@MicrobehunterMicroscopy
Its 4 am
Hey, I saw you use Canon camera on microscope to make footage. Is it possible to use Go pro camera in same way?
Gopro cameras gave a fixed wide angle lens so not really compatible when you need a telephoto/tube lens for your microscope.
How can I make a compound microscope at home with homemade lenses
I have a problem.I bought a 60x normal objective for my microscope. But it doesn’t work like it fits into the microscope but i cant see any slides but it works with every other objective that i have. The 60x is a bit smaller which could be the problem but why would that be? I just dont know what to do because i dont want to uae my oil obj.
What is the make and model of your scope ? What is the brand for the 60x objective ? Did you buy the objective new or used ?
Please list ALL of the text that is on the barrel of the 60x lens.
Regards, Gary S.
@@garyspencer1711 the lens read 60/0.85. bellow that is: 160/.17. The telescope that I bought was on amazon; "Swift SW350T 40X-2500X Magnification"
@@orestesc2803 The SW350T is a very good beginner microscope. So, when you install the 60x objective, does it thread smoothly into nosepiece ? Are you threading-in
the 60x objective completely all the way in ? Are you using the 10x EYEPIECES when using 60x objective ?
Do not use ANY eyepieces that are greater than 15x magnification. The 25x eyepieces that come with Swift and
AmScope microscopes are useless when using 40x, 60x, and 100x objecitves.
So, remember, ..........only use the 10x eyepieces while you are trying to figure out what is causing poor quality imaging with 60x objective.
Where did you buy the 60x objective ? What was the cost of 60x objective ?
If the 60x objective is SMALLER diameter than the other objectives, then it is not intended for STANDARD microscope and WILL NOT fit. It is for childrens TOY microscopes and cannot be used on your 350T microscope.
Also, like you, I do not like using 100x objective unless I need to view white blood cells (which is rare). Both of my
microscopes use: ____________4x, 10x, 20x, 40x, and 60x objectives. A few months ago, I purchased a new AmScope 60x objective for ( $57 USA + free shipping) and it is far better than I expected for ultra LOW cost. The remainder of my objectives are all Olympus.
If you buy a new 60x from AmScope, make sure you buy the 160mm type. Do not buy the "infinity corrected" type.
The 350T has standard 160mm tube length.
Gary S.
How can I make achromatic objective lens at home
Not possible unless you have the tools to grind and polish your own lenses. And this would be much more expensive than buying one. Check Aliexpress.com for microscope objectives. You can make yourself a single small lens by melting a glass rod over a gas flame.
At least 1000 x