Cryosectioning of Fixed and Frozen Tissue

This video describes the methods for cryosectioning fixed and frozen tissue in Optimal Cutting Temperature (OCT) using a motorized cryostat.
Visit this link (zenodo.org/rec... ) to read the written protocol which is open and freely available for download from Zenodo.
For more information about the work being done at The Neuro’s EDDU, please visit us at:
Website - www.mcgill.ca/...
Data portal - www.neuro-eddu...
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LinkedIn - / the-neuro-eddu

[@ijustrealllylikecats]

I have no reason to be watching this, I don't know why it was recommended. But I watched the whole thing bc it was super interesting. I had no idea how these things were done

[@bangminliu3262]

This is a good training video, especially for those beginners like me. Thanks.

[@paulchang8743]

Is it good to section the tissue without anti-roll glass? as what you did in the video?

[@MontrealNeuro]

Yes, this is called the brush technique. You can find an in-depth description of the brush technique on the website IHC World: A Method for Preparation of Frozen Sections (www.ihcworld.com/_protocols/histology/frozen_section_technique_1.htm).
When using this technique, the brush should be held like a pen and the hand can be stabilized by resting one finger on the stage. The pressure applied on the section when sliding it across the stage should be minimal as to avoid damage or the section adhering onto the stage. The brush should move at the same speed as the section sliding on the stage.

[@veterinarystudent9450]

Whether keeping tissue in paraformaldehyde won't lead to cross-linking