Hi, everyone! There's a typo in question four regarding the "S743L" mutation. Please read "S473" as "S743" for each mention in the question 4 stem and the answer choices. - Shemmassian MCAT Team
Exactly right - you're looking at what's shown in wild type to non-wild type (S743L). Based on the quantification of the Western blot (shown to the right of the Western blot in Figure 1), you can see that there's a significant decrease in protein levels for both the eye and scalp mutant OCA2 protein compared to WT. The asterisks indicate significance, which you likely wouldn't be able to tell just by looking at the Western blot (left panel of Figure 1) itself. Hope this helps!
You're absolutely right, Chris! Thank you for bringing that to our attention. We'll make a note here in the comment section so that other students know as well :)
For me, I am wondering why we don't consider there to be a significant difference. There is a difference, I'm sure you see, but how do you determine that it's insignificant if they don't give you the margin of error or some sort if que? Are you just eyeballing it?
Generally they'll note statistical significance with an asterisk on the graph and a footnote. The AAMC is pretty consistent about asking to you stick within the realm of the passage, so unless it's a very large, very obvious difference on the chart it's probably safe to assume it's not. This is just my experience and I'm not a pro though, so I could be wrong! This is definitely something I struggle the most with - I tend to make assumptions that aren't stated in the passage and the AAMC doesn't like that lol
Hi, everyone! There's a typo in question four regarding the "S743L" mutation. Please read "S473" as "S743" for each mention in the question 4 stem and the answer choices. - Shemmassian MCAT Team
Amazing video. Thank u for this, not many channels are making strategy videos like u guys!
Our pleasure!
Dude looks straight off "The Good Doctor", tell me that isn't a straight fact
Dr. Sean Murphy
So you recommend reading the entire passage before addressing the questions?
For question 2 where are you seeing decreasing for eye and scalp? Are you looking at a decrease from what is shown in wild type to non wild type?
Exactly right - you're looking at what's shown in wild type to non-wild type (S743L). Based on the quantification of the Western blot (shown to the right of the Western blot in Figure 1), you can see that there's a significant decrease in protein levels for both the eye and scalp mutant OCA2 protein compared to WT. The asterisks indicate significance, which you likely wouldn't be able to tell just by looking at the Western blot (left panel of Figure 1) itself. Hope this helps!
Pretty sure Question 4 has a typo. The gene in the passage being discussed is "S743L" and the question asks about "S473". Completely different.
You're absolutely right, Chris! Thank you for bringing that to our attention. We'll make a note here in the comment section so that other students know as well :)
For me, I am wondering why we don't consider there to be a significant difference. There is a difference, I'm sure you see, but how do you determine that it's insignificant if they don't give you the margin of error or some sort if que? Are you just eyeballing it?
Generally they'll note statistical significance with an asterisk on the graph and a footnote. The AAMC is pretty consistent about asking to you stick within the realm of the passage, so unless it's a very large, very obvious difference on the chart it's probably safe to assume it's not. This is just my experience and I'm not a pro though, so I could be wrong! This is definitely something I struggle the most with - I tend to make assumptions that aren't stated in the passage and the AAMC doesn't like that lol
Woah!!! I thought you were Shaun Murphy!
Stop codon mnemonic : I Are Annoying, U Go Away, U Are Gone.