Hello, SNPs in Sanger sequencing? Sometimes you can search for a SNP or Genotype by finding nested base calls. The base caller highlights the bases with higher intensities; therefore, it is crucial double check the chromatogram by eye.
hello, thanks for this nice and helpful presentation. Can you suggest how to create the consensus sequence of sanger sequencing (forward + reverse), after manual editing of the cromatograms? Is there a way to do it on finchTV?
Hello Simonazzi; Thank you for watching my video. Yes, there is a way. In this case you can use BioEdit to generate a consensus sequence out of the contigs (Forward and Reverse). You need to import froward and reverse sequence one-by-one, and align one to another (nucleic acid and reverse compliment) with pairwise alignment. In the newly generated window select both sequences and go to accessory application and create consensus sequence. I hope it works for you.
@@al1r3z49 ehi thanks, I was wondering if I can export the sequences edited on finchTV and then generate the consensus on bioedit, without have to edite them directly on bioedit (I don't like so much the editing features of bioedit). I've tried but apparently it doesn't work, so I guess I'll just bioedit :)
Hello, thank you for demonstrating it precisely. How can we identify SNPs in Flinch TV by observing the chromatogram?
Hello,
SNPs in Sanger sequencing? Sometimes you can search for a SNP or Genotype by finding nested base calls. The base caller highlights the bases with higher intensities; therefore, it is crucial double check the chromatogram by eye.
@@al1r3z49 Thank you
hello, thanks for this nice and helpful presentation. Can you suggest how to create the consensus sequence of sanger sequencing (forward + reverse), after manual editing of the cromatograms? Is there a way to do it on finchTV?
Hello Simonazzi;
Thank you for watching my video. Yes, there is a way. In this case you can use BioEdit to generate a consensus sequence out of the contigs (Forward and Reverse). You need to import froward and reverse sequence one-by-one, and align one to another (nucleic acid and reverse compliment) with pairwise alignment. In the newly generated window select both sequences and go to accessory application and create consensus sequence. I hope it works for you.
@@al1r3z49 ehi thanks, I was wondering if I can export the sequences edited on finchTV and then generate the consensus on bioedit, without have to edite them directly on bioedit (I don't like so much the editing features of bioedit). I've tried but apparently it doesn't work, so I guess I'll just bioedit :)
@@marameosimonazzi1180 I think you actually can import trimmed fasta seq from FinchTV to Bioedit. You just have to import sequences.