Making Luminol
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- Опубліковано 7 лют 2025
- Hey guys, this is the last part of the luminol synthesis. It has been a long time coming and I am sorry for the mega delay!
Luminol Recipe from: www.chm.bris.ac...
3-nitrphthalic acid synthesis video: • Making 3-nitrophthalic...
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Nile talks about lab safety: • Chemistry is dangerous.
Great! Now I can make luminol and test if I cleaned the blood enough.
Riddim Dubstep 😂
Muriatic acid is pretty good at cleaning blood from semi porous surfaces like concrete, but it will also attack the concrete. After that sodium hydroxide can be used to destroy trace proteins.
@@nunyabisnass1141 thanks! Exactly what I needed ;-D
Jimmy Tremblay yeah just so we're clear, you're a hunter, and you hang the carcass in your garage...right? Lol
@@nunyabisnass1141 call me a... contractor... of sorts...
I do hang the carcasses in my garage, thanks for asking :-D
excellent work! way better and cleaner than my approach.
+NurdRage Damn, that means a lot coming from you. I also think this is the first time that you've commented on a video of mine :)
+NurdRage you guys should do a collaborative video together! That'd be amazing to see!
+Nile Red It's funny thought that NurdRage was you and you were saying that your approach was better than... your approach. It's the NR initials. ;)
+Bane? NR means No Reaction
+NurdRage I totally read this in your voice!
I don't know how anyone can dislike Nile's videos they're great, interesting, educating and entertaining
Someone who doesn't understand the chemistry and can't appreciate the effort he puts in.
@@Hezarou or they don’t like the editing style, lack of closed captioning etc. not everything is black and white and not everyone has to like everything you do
@@cadenbrown7407 what? No, I'm not talking about appreciating the work he puts in; the effort. You can dislike the style, the captions, hell you can dislike Nile himself. But you can't deny he does what he loves and he puts effort in, I always appreciate people who put effort in what they like. So don't think everyone you meet online is as shallow as you believe they are.
It could also be possible, that people accidently klick dislike. I also had it a few times, that I opened a video and I suddenly saw, that I disliked it before accidently without noticing🤔
I can't imagine, that anyone would dislike this!
@@Hezarou You totally missed the point and reiterated a prior opinion. Excellent comprehension.
I really hope you do a video of luminol reacting with blood. I am currently working towards a Forensic Science major at Wichita State University, and am highly interested in chemistry as well, so I'd love to see a video that applies to both :D
You still in Wichita
@@HooyahPeacock I sure am.
Me too
@@HooyahPeacock That's cool. I'm actually still at WSU for forensic science. I'm getting my bachelor's at the end of the year.
@@AmusedToast COME TO LOS ANGELES, WE HAVE PLENTY OF COLD CASE FILES TO SOLVE, INCLUDING Y FRIEND LUCIA, WHO SUPPOSEDLY SHOT HER SELF IN THE HEAD WITH HER SHERIFF BOY FRIEND, HE WAS ALWAYS A TOTAL JERK OF A GUY, AND I NEVER TRUSTED, OR RESPECTED HIM AS A PERSON. LUCIA HAD A TWO YEAR OLD, THAT SHE LOVED TO DEATH, I KNEW LUCIA WELL, LEA REMEDY JUST MOVED TO HOLLYWOOD SCIENTOLOGY, TO PURSUE HER ACTING CAREER, AND STARTED TO HANG OUT WITH US AT LUCIA’S HOUSE, AND GOT TO KNOW WHAT A JERK LUCIA’S SHERIFF BIY FRIEND WAS. I BELIEVE IT WENT DOWN AT HIS HOUSE IN STUDIO CITY. RIP LUCIA BENTTONCOURT.
If you ever make t-shirts, one idea is to have the words, "We'll just have to pretend that it's there."
i think an "anyway" shirt would be awesome too lol
"ice old ethanol" tshirt would be the best lol
"Wash it with distilled water"
"In hindsight, I should have used a larger container"
@@krissp8712 "the yield is extremely low, and I don't know why"
I like seeing your mistakes much more than successes. Thanks for keeping mistakes and sharing them with us.
Luminol produces a nice blue glow.
Your second channel is "creatively called" Nile Blue.
I think you should create a NB logo with luminol just like you did in this video with NR.
+Melody Williams That is actually a pretty good idea!
is this truly the comment that gave birth to the nile blue idea?
Thanks!
Have a coffee
Clearly opaque... I see what you did there...
I am a wordsmith
krap101 i was about to comment this!
😭
Why is there a random Australian dude that cuts in at 15:47?
lol
Idk!
LMAO
I think it was just a voice taken from another video as he said 25 grams of what he was adding and then Nile repeated that.
Don't Even Bother
IT’S EXTRACTIONS&IRE
ecstasy.apostasy ITS ALSO EXPLOSIONS & FIRE
I did this synthesis last week. From 200g of phtalic anhydride (pure p.a., Acros) i got 40 grams of 3-nitrophtalic acid. I took 10g of acid which yielded about 8g (6g after drying in oven) of luminol. All steps were identical, except I used pure sodium dithionite, cause it can be bought over the counter in Poland. Only last step was a bit different - I dumped the sodium dithionite to the solution, brought it to a short boil, and left it to cool. Great video.
It is 2:30 am December 2021. I've finished work, made the long drive home and I have chosen this video to watch. It's a 5 year old video at this point with 19 minutes of a pair of hands making a forensic liquid because I remembered how cool it looked on CSI.
I can’t tell you how refreshing it is to see a video presented by someone that knows what they’re talking about, is clear and concise without the unnecessary theatrics you see when people are trying to turn themselves into a social media personality and/or sell you something. I definitely would be open to more detail/instruction on the reaction mechanisms.
How much nilered has grown in the years is crazy, great job, keep it up
Tasty mango smoothie
Edit: Lemonade looks good too
glowing gatorade
why couldnt my chemistry teacher make it this interesting i would've actually paid attention in class
Cause they don't know what they talking bout
I follwed this procedure and was able to get 1.33 grams of luminol in my chem class, thanks nile red. But the oxidation didn't work out for me cause of lab misinterpreting ferricyanide with ferrocyanide so had to use bleach for that. I loved this experiment.
Thank you so much! I had to find an original experiment for college o-chem and this is so helpful!
I'm still waiting on the future video of you exploring luminol. Its been long enough Nigel.
I actually first learned about this chemical from playing ace attorney! So cool to see how it is made
same 😩
The color it has when it first glows is my favorite color ever. I absolutely love that color.
I've just discovered your channel and I'm completely blown away. This is proper hands on chemistry, a clear demonstration of various chemical reactions resulting in a variety of products using simple language that is accessible to anyone. In chemistry classes these days it feels like so much time is spent trying to get students to think in abstract terms about theory that they rarely if ever get to experience genuine practice. In my opinion this approach to learning is backwards; you shouldn't try to teach someone to do something before you do it, you should walk them through and SHOW them the process in action, and then seek to explain it in detail!
Mrjmaxted0291 yeah i completely agree
Isn't Hydrazine a fuel used in spacecraft reaction motors?
Yes
I want you as my chemistry teacher!
Followed this synth, one of the coolest things I've done.
Just want to let you know this is my favorite UA-cam channel.
That voice at 15:56 reminds me of "2 shots of vodka".
AND THERE YOU HAVE IT
I love that you're using pieces of old papers you printed, been there.
Luminol is so cool and interesting! Thank you for making these videos!
I'm no chemist, but what I do know from other endeavors is that having your thermometer directly in contact with your beaker during heating might not be the best idea.
It has to introduce some variance due to the thermal nature of the glass. I'd think you would want the business end of the thermometer in contact with nothing but the solution you're monitoring.
I guess since there is a temp. range in this reaction it isn't that critical... But I've found that using best practices habitually ends up being better in the long run.
11:01 looks like slightly off orange juce
I'd drink it
Pete Lorenzo i wouldn't
sunnyD
The deadly orange juice...
Mmmmm refreshing
this is my favorite video of yours nile! i love forensics :)
Ah, the old heating the test-tube with a heat gun trick.
Had to do a diphenylacetylene and then hexaphenylbenzene synthesis recently which involved exactly that, except with little to no solvent.
I had to synthesize luminol for my final practical assignment in my oragnic chemistry lab last semester! The chemiluminescence lasted quite a while and was super bright! I got a good grade too :)
It's been 4 years. Did you forget about the luminol and blood experiment? I can't find it on Nile Blue either.
Got interested in the synthesis playing Trauma Team and thought to myself "NileRed should synthesize and test luminol" then it turns out he already did! What a treat
Fantastic results man!
Vacuum filtration looks so satisfying.
Awesome video as always nile
it was pleasure to hear your real voice !!!
+Rajas Godbole but I always use my real voice?
+Nile Red He was referring to the voice that said "25 grams of sodium bicarbonate". Sounded Australian
Ludo Kressh haha it was my friend (who decided to put on an accent)
lol
Next up: Synthesis of fluoroantimonic acid! Please.
Ain't that the most corrosive acid in the world?
+MrMatnor2403 yup.
You're going to need a lot of teflon. Good luck.
Teflon: I'm ready baby!
Sodium dithionite is only metastable and rapidly decomposes in acid. My guess is the household version has the bicarb as a stabilizer. That's also why you get it as a possible range. Even the chem suppliers sell it as such and you usually use an excess. Again, nice hands NileRed. Mechanism is off, consider the first step going through the anhydride of the diacid (made by dehydration under the high heat). Second addition would also go through an anhydride or ester amide intermediate (from your glycerol or a dimer of the acids). Excellent demonstration.
I can't understand shit about chemestry but I still enjoy watching Nile's videos
Some quick questions about this synthesis:
1) Why did you use acetate to free the hydrazine? Wouldn't hydrazine (bp 114 C) boil off before the acid (bp 119 C) does? Chloride seems like it would accomplish the same goal, except it is even easier to obtain and has a lower boiling point. It also seems like the acetic acid and hydrazine might have potentially reacted to acetylhydrazide, hurting your yield.
2) Why was the nitro reduction done after the hydrazide was formed? The basic conditions are capable of hydrolysing hydrazides.
These videos get me through my anxiety attacks
Buy a cheap ceramic coffee mug, break a few pieces off and crush until the right size, the porous ceramic makes for a good replacement for boiling stones.
+jdflyback That is very true. I am just lazy :p
I guess iron ion in Hemoglobin acts as catalyst when it comes into contact with hydrogen peroxide and Luminol mixture.
Doing a lab report without doing the exp so I’m glad I get to see what I could’ve done
@NileRed great interesting video, maybe i'll try it myself..... something else i wanted. You're using mainly magnetic stirer that means you don't have to have boiling stones but from my experience it's better to have boiling stones. Mainly for destilations and methods where is required lot of heat. My magnetic stir hotplate is not efficent as yours because of that i'm using old ETA spiral hotplate (slovakian thing) , it's capable of much higher temperatures (sometimes really crazy) as my magnetic stirer. Well in that case i need boiling stones. At school we're using broken porcelain plates but i personaly prefer ceramic filtration rolls from aquarium filter because they don't react and have much more surface than porcelain fragments. Thanks that i can use much higher temperatures without bobbling out.
Wait a minute. If the max amount of the other chemicals is 66,5% how can the sodium dithionite be present in only 15% concentration?
Brovo! Some of your best work. :) keep it up!
That orange colour looks delicious
love it once again
Whenever you put it in the vacuum filter it looks like a tasty mango smoothie!
Probably shouldn’t drink it though..
I make my boiling stones out of broken glassware. I just take a broken beaker, or flask and smash it into 1-2 inch pieces; then I heat it, in a torch flame, until it is amorphous and then crimp it with pliers.
I repeat this procedure until it looks like a lumpy sphere and then heat it a bit more to polish any edges. It is a pretty cost effective use of damaged glassware, which we all have more of than we would like =/
Using this for my lab practical in 2 hours. 🤩🙏
All I watch is forensic files and this is on every episode!!!!
6:50 Why doesn't the free base Hydrazine boil off along with the Acetic acid? Both compounds have a boiling point around 114 C :/ If we increase the temperature to 200-220 C shouldn't we lose all of the Hydrazine?
Hydrazine acetate is soluble in glycerin. At the heat of about 220°C it decomposes into hydrazine and acetic acid. The hydrazine is soluble in the glycerin, thus keeping it from evaporating off, the acetic acid isn't(or very hardly soluble), so it just boils away.
Oh i didn't realize that. Thank you! :)
Not that palladium on carbon is easy to come by, but in theory, you could have used hydrazine sulfate to reduce the nitro group as well, but as I've never run such reactions in glycerol, I can't say for certain if this could be converted to a 1 pot process.
15:53 what? Random australian?
*new zealander
Way too nasal to be a kiwi!
I have read the masks which drop down for emergencies on a jet are a chemical reaction to produce oxygen. I would love to see a reaction which produces "potable" oxygen.
I always call heat guns industrial hairdryers. It make all the wood shop jockeys angry.
Woo! You finally made it!
I've been wanting to make Zinc Sulfide to play around with phosphorescence. I tried it the other day and didn't have a black light handy to test it out, but I don't think it worked because it didn't pop like fireworks when it burned (and the product was black).
+Jesse Downing hmm, where did you get your reagents?
Nile Red I'll have to look and see where they originally came from. They've been sitting in our supply closet for years.
***** Yeah they might not be the most pure
My brain - "Mango juice? Drink it."
My brain - “original commenter breedable? Breed him”
Who said "25 grams of sodium bicarbonate" at 15:47? Sounded Australian or maybe South African. Was that you NileRed; do try to speak with a N.American accent on your vids?
+E2qNX8btraQ3zRD6J7fc haha its my friend. I thought i was funny so i kept it
I was half asleep when I heard that, and it scared the shit out of me.
E2qNX8btraQ3zRD6J7fc I think he's Canadian. On his ethanol from the fermentation of sugar video, the sugar he used had both English and French text, but I wouldn't be sure about that.
Edit: I didn't notice at first, but there's also some bilingual text in the rust stain remover at 2:15
Felix the Sloth also his site is a .ca domain
Im australian.. and that was definately an australian accent 100%..
please make a video on stilbene derived optical brightening agents
hey Nile, I've been wondering- since glowsticks are a chemical reaction can you reverse it or I should say extract the luminal for reuse(if so how difficult would that be). thanks.
glowsticks dont use luminol or else why do they glow for more than an hour. we saw that it only lasts for about a minute so that's a stupid question
That heat gun seems super useful.
The introduction of the heat gun
TFW you go to watch a video making luminol, then suddenly realize you've stumbled upon the Chemical Avengers entry in some weird chemistry extended universe and now have to watch a dozen other videos to see what you missed.
Back in the early 1970's when I was in junior high school there was an (I'm guessing 1950's vintage?) chemistry experiments book in the school library that had a whole chapter on Chemiluminescence. It gave multiple formulas including this same one, and several others such as a deep red light made using Pyrogallol. As sources for the chemicals it listed Kodak, and also a Varniton Company located in Burbank California as I recall. Some other experiments shown were making pyrophoric lead, and dissolving cellulose then extruding Rayon. I'm just giving a couple examples from memory to see if maybe this rings a bell with anyone that may have the book or know the title and publisher info? I've always wanted to find a copy of this book, or to at least get a photocopy of it. Does anyone know of it? Thanks in advance.
i never get tired of seeing things change color and get vacuum filtered 0-o
Did you even add the ammonium carbonate?
I did not see you to add it. I hope you to reply me soon, please!
I’m kinda late but he did at 7:50 :)
It's at 16:00. The 7:50 annotation is wrong, it's just water.
If luminol reacts with the iron in blood, does someone's blood who has anemia react less?
no likely not. there are a lot of false positives with luminol though like bleach and horse radish
Even someone with anaemia still has easily enough iron in her blood to be detectable so while the answer to your question is yes, it wouldn't be noticeable and wouldn't affect detection rates.
@@meiyuan2134 just about any oxidizer will work
I don't care about the science. Im just here to see Wizard Nile mix stuff around and brew potions
The best boiling stones on the planet that work perfectly every single time are small the regular chunks of PTFE. Do it I did simply go down to a metal supplier as most sell Plastics to and buy a scrap junk of Teflon and cut it up. I use the bandsaw cuz I have one but you could use a little hacksaw or anything it just chop it up clean them in hot sulfuric acid and you're done. On the bandsaw I made about 2 cups worth of tiny 5 mm Square boiling stones in probably less than 10 minutes. I've have never had a bump since
NileRed working with hydrazine: "We got some splashing, but that's okay."
15:00 heh I made a video with those exact ingredients yesterday. I didn't know you made one :P
What other reducing agents do you think would work in the final reduction to make Luminol? Do you think sodium borohydride could reduce the nitro group?
Excellent videos.
Either you're part Australian... Or you were watching an E&I video while editing this and didn't realize you included his voice.. lol
Old video, but... i'm binge watching all your videos... how do people come up with reactions like that ? is it just luck ? do you spend hours in the lab just mixing stuff together and see what you get ? or is it possible to really know in advance what you want to create and just do it ? so many complex reactions (not only in this video) sometimes I wonder if you guys just mix stuff up, then try to come up with a use for the product after the fact ! hehe
Most everything in chemistry has been around for a long time and was discovered long ago by chemists who didn't know what would happen, and sometimes they didn't even know the elements that made up their products because they hadn't been discovered yet. They just knew that the reactions worked. Take for example the discovery of nitrocellulose (gun cotton). That was discovered by accident when a chemist spilled nitric and sulfuric acids and cleaned them up with a cloth and hung it out to dry, eventually bursting into flames and upon further investigation the product was discovered and named. A lot of stuff was discovered by accident like that. These days, we can predict, for the most part, what the reaction products and side products will be as we now clearly understand the periodic table of elements and how they react with one another. Simply typing the precursors into google and hitting enter will give the reaction and its products, so now a days we don't even have to work to figure it out. So yes, we can know in advance what (for the most part) our reactions will create these days. That, and chemists like to tinker and play with reactions to see what happens. Which often leads to discoveries. Curiosity is the driver of innovation and discovery.
I propose a NileRed drinking game: every time he mentions that the he had to transfer the mixture to a larger beaker, drink one shot of tequila; when he mentions he could have done a gravity filtration but chose to do ir faster by using vacuum, two shots; when he mentions the whole thing started to become a nightmare/a disaster at some point, three shots!
7:49 why does the caption of this step say "Added 0.2g ammonium carbonate" if he is mixing distilled water into the beaker?
Um.... What was that at 15:47 ? Is this someone else's video? I heard an aussie voice and could hear the stir bar clicking, which leads me to suspect that this is not actually you preforming this reaction. Do you have an explanation?
+Nakor Isilani haha, it's my friend. He is British though, imitating an Aussie accent
Ahh.. I see. Is he the one adding the sodium bicarbonate?
Basic solutions: the tears of a person who's Pumpkin Spice Latte order was wrong
Or
The blood of a person who cut themselves with their exact-o knife when opening their slime package
Both of these have a pH of around 14
Enjoy your basic life
Is there any other way of reducing NO2 to NH2 by something else except of sodium dithionite? In my contry is not widely available :/
12:51 bit it’s color is neutral color for your ph indicator (you made a ph indicator
Thanks you for the information. Now what's the best solution to dissolve meat and bones? I have about 200 lbs of extra turkey left over from tha thanksgiving
"Turkey" 😂
God.. Beautiful craft. This one lost me atm.. Keep em comin Dude
Ema Skye: WRITE THAT DOWN WRIGHT THAT DOWN!!!
10:47
>and the solution becomes clearly opaque
+Name Namerson Oxymoron :)
why didn't I get a notification for this one?
I could have missed it!
(phew)
hi 5 years later
"Clearly opaque"
I like what you did there... 😁
If you start at n2h to nh2 before the vacuum pump would you be able to fallow the rest of the step as is or would it turn out a bit thicker or unusable
Oh no, my brain says 8:10 looks like the best smoothie
"it becomes clearly opaque"
Who’s voice was that at 15:47 (25g of sodium bicarbonate)? It sounded like an Aussie dialect.
+fpm1979 haha I forgot to put in an annotation. It is my friend doing an accent.
Cool glow in dark acid
So I’m deciding to try to make luminol for my science project. *Boy, I don’t know what I’ve just done.*
It's that explosions and fire @15:47
No