Karolin Luger (CU Boulder, HHMI): Discovery of the Structure of the Nucleosome
Вставка
- Опубліковано 22 лип 2024
- www.ibiology.org/genetics-and...
Dr. Karolin Luger shares her discovery of the 3D structure of the nucleosome.
The nucleosome is the central organizing structure of the eukaryotic genome. It consists of DNA wrapped around histone proteins. Dr. Karolin Luger shares her discovery of the three-dimensional structure of the nucleosome using X-ray crystallography, which provided a deeper understanding of chromatin organization.
Speaker Biography:
Karolin Luger earned her bachelor’s degree in microbiology and her master’s degree in biochemistry from the University of Innsbruck. She completed her PhD in biochemistry and biophysics at the University of Basel. In 1997, she published the first high-resolution 3D structure of the nucleosome, determined using X-ray crystallography. The Luger Lab uses structural biology to understand the impact of chromatin architecture on fundamental genome-related processes.
lugerlab.org
0:00 Intro
0:34 Complex organisms call for complex genomes
1:10 Large genomes pose many challenges
1:52 Early views of genome packaging: the nucleosome
2:41 X-ray crystallography: the method of choice for structure determination in the 20th century
4:17 ‘High resolution’ is needed to understand function
5:52 Resolution is determined by order
6:47 ‘Natural’ nucleosomes are heterogenous
7:49 1984: 7 Ångstrom structure of the nucleosome
8:55 The path toward higher resolution
9:55 Recombinant nucleosomes
11:28 Two steps forward, three steps back
14:03 What is going on? DNA position on the histones is not uniform
16:20 Structural biology is an endurance sport
17:22 Data collection at 3rd-generation synchrotrons
17:55 The Results
Credits:
Brittany Anderton (iBiology): Producer
Eric Kornblum (iBiology): Editor, Videographer - Наука та технологія
How cool is it to have the actual research scientist explain their journey! Just amazing and inspirational.
The lecture is fantastic, clear, easy to follow, intriguing, vivid, and informative.
!!!!! iBiology, thank you so much for all the TOP vídeos you deliver to all of us !!!!!
Yeah they are just amazing ❤️❤️
🤝
Amazing presenter, superb content, crazy good!!!!!!!!!!!!!
Studying epigenetics and transgenerstional inhertance this semester. Fell in love with the structure the moment i saw it.
I think Dr.Luger deserves a nobel prize for such a painstakingly tough and seminal work.
Bro you are fit and smart how do you manage it
Thank you Prof. Karolin Luger for this amazing and interesting lecture !
💯
I can also feel the hard work they put it into just listening.so cannot imagine how hard it must have been.
thank you Dr. Karolin Luger !
Brilliant technique..i feel wowed!
Hey there great content and very informative 👍
Thank u very much for the knowledge.who would have thought to use synchrotron to map dna.great job scientist.
Thanks that was helpful
Nice
That heating experiment was geneius
Just 👍 great
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