Concentration of Glucose in Fake Urine. Colorimetric Techniques (A level but not AS)

AQA A level Biology Required Practical can be carried out to identify concentration of glucose in fake urine or any other liquid. Fake urine can be produced using cold tea with added glucose in the urine from a patient with diabetes.

[@tulasivaibhavi4246]

16 year old from singapore here. I did this exact test for my preliminary examinations and i aced them. Thank you so much!

[@KingslinaOboh]

That's nice
Congratulation on your success

[@reecesaxton8115]

could you please complete all of the AQA AS/A2 practicals ...your videos are so useful especially for paper 3 revison! thankyou!

[@hanaz4954]

could you try to update your youtube channel with the new syllabus

[@kesheekabhookhun2081]

Could you please videos on how to use microscopes and different a level experiment using it?

[@katherineleong6905]

I'd like to ask why you join the points with a line point to point instead of a line of best fit?
Also, why does it level off near the bottom?
Can you actually measure the intensity of red light instead (so without filtering the red ppt)?

[@plantinpot5712]

Thank you.x

[@finndooley7019]

What would the dependent and independent variable be?

[@Syiepherze]

Would you get more accurate results if you used yellow light instead of red light?

[@ThayefK]

What’s your name
Need it for bibliography?

[@muhammadvohra5187]

Instead of using absorbance of light in the colorimeter, can you use transmission? Also if it does work then what kind of changes will it have on the calibration curve?

[@biologypracticalsandrevisi8066]

Muhammad Vohra yes it will work fine. The curve will just be reversed.

[@Yo-pm7zo]

At the end of the video you said that the fizzy drink(sprite) is 1.75 what if it was 2.5 , how would you find out its level of concentration of glucose ?

[@biologypracticalsandrevisi8066]

Beautiful Potato You could dilute your fizzy drink by 50% and carry out a new Benedicts test on it and read off the concentration from the calibration curve then double it.

[@MsShubhammalik]

i dont understand how you calculated the amount in ml of glucose and water to be added for each dilution?

[@FlyingPotatoPie]

I normally just use the equation (concentration 1 x volume 1) = (concentration 2 x volume 2).
For example for a 2% solution: the concentration you want is 2/100= 0.02 and you want a 10cm3 volume of it.
(0.02 x 10) = 0.2
You want to find the other volume but you know the concentration, so if you rearrange the equation all you have to do is divide 0.2 by the concentration of the actual glucose solution which is (10/100) 0.1.
0.2/0.1 = 2 so you need 2cm3 of the glucose solution but the total volume has to equal 10cm3 so 10-2=8 leaving 8cm3 of water.
Hope this helps, took me a while to understand it too. xxx

[@MsShubhammalik]

Thank you so much!! :)

[@shreychandran5208]

I wanted to ask, what is it what they test on paper 3 for aqa a level as in what type of questions would they ask ? Would it be to questions about why you use certain equipment and control variables etc?

[@biologypracticalsandrevisi8066]

Shrey Chandran you need to look on the aqua website where past papers and specimen papers are available. Paper 3 has the essay and questions could cover any topic but some relate to practical skills plus there is always a data question where you need to be able to use the results of statistical analysis to draw a conclusion about significance of results. It is early days for the new aqua spec so your best bet is to look on their website.

[@DanielaRodriguez-qt3pv]

What is the concentration of the glucose solution?

[@tarrystyles]

Is it a quantitative test or qualitative?

[@biologypracticalsandrevisi8066]

tanya shah Quantitative as it gives a numerical value. It tells you the vile contraction of the solution.

[@AboveAgeing]

Good content of the video but she sounds angry and it's a little off-putting haha

[@urmomsah0e476]

i love you THANK YOU