External calibration

This video explains how to determine the concentration of an unknown solution using external calibration.

[@TheJoshtheboss]

Excellent job! Any video on Internal Standards?

[@lindsayharding8930]

Not yet but I’ll work on it, thanks for the suggestion!

[@TheJoshtheboss]

@@lindsayharding8930 Thank you.

[@ebrahimmoto]

I like this thanks allot

[@josenjogu8896]

Thankyou

[@oliseemeke1459]

What if the GC areas are observed at different retention times, do we still take the average of the areas?

[@AnalyticalScienceTutor]

If the peaks are at significantly different retention times, under the same experimental conditions, then they may be different compounds, so can't be averaged.

[@adhixxsanju]

Mam for calibration also do we need to take the sample in triplicate runs??

[@lindsayharding8930]

Hi, yes, ideally you should run everything at least three times.

[@adhixxsanju]

Can you please provide me your mail id I have some queries??