Numerical Aperture

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  • Опубліковано 22 гру 2024

КОМЕНТАРІ •

  • @PunmasterSTP
    @PunmasterSTP 8 місяців тому +4

    I came here after hearing about the high-NA EUV lithography, and this was a great explanation of the core concept. Thank you so much!

  • @SJMNin
    @SJMNin 6 років тому +11

    Clear explanation, simple and scientifically rigorous. Looking forward to the next video

  • @tymothylim6550
    @tymothylim6550 4 роки тому +5

    Wow that is a great video :) Thanks for the great effort put in.

  • @abinayaaaaaaaa
    @abinayaaaaaaaa 6 років тому +4

    Please make more such videos on basics of microscopy

  • @livviibb
    @livviibb 5 років тому +2

    these are such a good series of videos!

  • @chriforrer
    @chriforrer Рік тому

    I kindly suggest you to do a video about the entrance and exit pupil, and the aperture and field stop as well, of an optical system. Might be presented in a more linear and clearer way than how I was taught those concepts in the first place.

    • @Microcourses
      @Microcourses  Рік тому

      Check out my video on microscope alignment. It may be what you’re looking for!

  • @dcamron46
    @dcamron46 2 роки тому

    It’s also a property optical fiber, how does the definition vary in this as compared to lens? Why doesn’t the diameter matter

  • @allenshaw9996
    @allenshaw9996 Рік тому

    Are there any downsides to using higher numerical apertures?

  • @mp3lwgm
    @mp3lwgm Рік тому

    Your equation for d makes it larger than the Abbe diffraction equation. According to Abbe the correct number in the numerator os 0.5 and not 0.61.

    • @Microcourses
      @Microcourses  Рік тому

      The equation I’ve given is not mine. It’s the Rayleigh equation, which uses a different criterion than Abbe for defining resolution. Rayleigh criterion is the most commonly used and accepted.

  • @serdaraliandrnloglu3220
    @serdaraliandrnloglu3220 2 роки тому +1

    For those who want to know what what is the reyleigh criterion and why is it defined like that, I suggest you to read fraunhofer diffraction chapter from the book Introduction to Optics by Frank L Pedrotti, section 11-3 Rectengular and Circular Apperatures.

  • @niteshgupta4356
    @niteshgupta4356 5 років тому

    what are photo Bleaching and Photo Toxicity that you have mentioned in your video

    • @Microcourses
      @Microcourses  5 років тому

      Check out Laissue, P. Philippe, Rana A. Alghamdi, Pavel Tomancak, Emmanuel G. Reynaud, and Hari Shroff. 2017. “Assessing Phototoxicity in Live Fluorescence Imaging.” Nature Methods 14 (7): 657-61.

    • @alexandrevaliquette3883
      @alexandrevaliquette3883 5 місяців тому

      If you are not autistic, the proper answer would be: too much light will damage your specimen. In fluorescence, if the sample get too much light, it won't emit as much. So you are 'killing' your sample and you cannot see the light it emit.

  • @randydaumar4667
    @randydaumar4667 4 роки тому

    I have a probably dumb question:
    Does the ocular lens have numerical aperture?

    • @Microcourses
      @Microcourses  3 роки тому

      Yes, every lens has a limited capacity to collect light and therefore has a numerical aperture.

    • @randydaumar4667
      @randydaumar4667 3 роки тому

      @@Microcourses however, it is never specified, right?

  • @salmansiddiquekhan3388
    @salmansiddiquekhan3388 4 роки тому

    How does it affect resolution?

    • @Microcourses
      @Microcourses  4 роки тому +2

      Resolution improves as NA increases. NA is in the denominator of the resolution equations. www.microscopyu.com/microscopy-basics/resolution

  • @aarohimehendale8333
    @aarohimehendale8333 2 роки тому

    Great lecture. Thank you! -Aarohi, Mack, and Mohammed

  • @achamess
    @achamess 6 років тому

    Excellent video. Very clear and practical.

  • @dancedancebroccoli6485
    @dancedancebroccoli6485 3 роки тому

    Great video! Thank you.

  • @yizhou866
    @yizhou866 4 роки тому

    Thanks for your helpful courses

  • @gangadharsatyanaga
    @gangadharsatyanaga 5 років тому

    awesome, plzz make more videos

  • @pioneercolonel
    @pioneercolonel 3 роки тому

    Thank you!

  • @sandeepsah4937
    @sandeepsah4937 4 роки тому

    Very helpful video

  • @GeorgeC1andonly
    @GeorgeC1andonly 5 років тому +3

    Don't oil an air objective.
    Every month I clean at least one!

  • @aatt3209
    @aatt3209 3 роки тому

    excellent!

  • @mehakrai6840
    @mehakrai6840 4 роки тому

    Thanks ❤️

  • @lokeshranjanjha1796
    @lokeshranjanjha1796 5 років тому

    thanks

  • @goombaboomba593
    @goombaboomba593 Рік тому

    Shiiiiiii tally with the clutch

  • @vijaybenz9741
    @vijaybenz9741 2 роки тому

    Jenifer Walters attorney at law