Uma coisa que eu acho diferente é que vcs fazem PCR fora do fluxo. Aqui eu faço as minhas todas dentro de fluxo laminar. E tem 3 salas separadas. Uma para montagem da PCR ( reagentes) uma pra colocar o DNA na reação de PCR e uma pra correr. Usamos a estante prós tubos congelada assim mantemos melhor os reagentes durante as montagens de PCR
Yes that's true, I think many labs would do it like you, but we only have one communal one shared between multiple labs and since I do lots of PCRs, it's easier to do it on the bench! Ofc I make sure to clean the surface thoroughly before starting.
I did a placement this summer and really struggles with calculations like the master mix I worked under a postdoc in a base editing project which consisted of many cloning & it involved so much calculations and conversions does this get easier the more you work in the lab
Oh it definitely gets easier with time and practice! My first few times doing all sorts of calculations whether it's serial dilutions, master mixes, calculating reagent volumes for cloning/Gibson assembly, were all confusing. Once I started to get the hang of it and understand the role of each reagent in the process, it starts to make so much more logical sense!
This really motivates me to keep making videos, thank you 😊 I graduated with a 1st class (~70%) in undergrad and Distinction (~70%) for my Master's. One major piece of advice I'd tell myself is to be less of a perfectionist when it comes to taking notes and revising for exams. University exams do have knowledge based questions but I'd say it's more important to understand the principles and how things tie together. I would've focused more time on linking information tgt using mind maps and also actively recalling topics I was worse at rather than going through everything in sequential order - it's pointless to revise something I already know 5 times 😂 These are great questions and I'll try to make a more detailed video on this.
YES WOMEN IN STEM 🤩love to see it!
yaaass ❤❤
Uma coisa que eu acho diferente é que vcs fazem PCR fora do fluxo. Aqui eu faço as minhas todas dentro de fluxo laminar. E tem 3 salas separadas. Uma para montagem da PCR ( reagentes) uma pra colocar o DNA na reação de PCR e uma pra correr. Usamos a estante prós tubos congelada assim mantemos melhor os reagentes durante as montagens de PCR
Yes that's true, I think many labs would do it like you, but we only have one communal one shared between multiple labs and since I do lots of PCRs, it's easier to do it on the bench! Ofc I make sure to clean the surface thoroughly before starting.
I did a placement this summer and really struggles with calculations like the master mix I worked under a postdoc in a base editing project which consisted of many cloning & it involved so much calculations and conversions does this get easier the more you work in the lab
Oh it definitely gets easier with time and practice! My first few times doing all sorts of calculations whether it's serial dilutions, master mixes, calculating reagent volumes for cloning/Gibson assembly, were all confusing. Once I started to get the hang of it and understand the role of each reagent in the process, it starts to make so much more logical sense!
i love this !!
This really motivates me to keep making videos, thank you 😊 I graduated with a 1st class (~70%) in undergrad and Distinction (~70%) for my Master's.
One major piece of advice I'd tell myself is to be less of a perfectionist when it comes to taking notes and revising for exams. University exams do have knowledge based questions but I'd say it's more important to understand the principles and how things tie together. I would've focused more time on linking information tgt using mind maps and also actively recalling topics I was worse at rather than going through everything in sequential order - it's pointless to revise something I already know 5 times 😂
These are great questions and I'll try to make a more detailed video on this.
@@biomedwithvy thank u so much! i look forward to watching any vids if u do decide to post
Hi is it necessary to take hcpc registration to work as microbiologist?keep upload videos, very nice lab videos 👏👏
No I do not think it's necessary, or at least I didn't need to to do microbiology work in the lab. And thank you!! 😊