Germinated Mushroom Spores on Agar - Day 5 plate read since breeding mushrooms from spores
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- Опубліковано 28 гру 2021
- Day 5 into our 2022 breeding project we have our first germinated spores! Stay tuned as we take new phenotypes for the next season and walk through cleaning up contamination, selecting different mycelium and choosing the best substrates for our mushroom farm!
Original inoculation video here: Immaculate Inoculation FIXED: kicking off the 2022 mushroom breeding project (germinating mushrooms) • Immaculate Inoculation...
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Once figured out and done right mushrooms are very easy to grow and actually fun to grow! I keep keeping spores and cultures and its a never ending cycle of growing. Love growing on agar!!!!!!!!!
It's pretty obvious you are elevating the game for all of us, thank you!!!
Good video buddy, keep up the great work, and MushLuv to my mushroom family!
Thanks!
Very clarifying video, thanks!
Props for writing backward and in reverse on the back of that dish.
It's pretty obvious to me now that we can all learn from you're organization skills just as much as we can from you're myco genius. Are all of your plates stored at the same temperature?
72F incubation and 37F for long term storage - I usually pull them out and let them sit a few hours at room temp before doing culture work to help reduce condensation
hi thanks for the video. im looking forward to the peroxide treatment. im always questioning myself about how long things should take so getting these updates is really good info for me!
Very helpful, thank you.
Thanks Gary...exactly the video I needed. I inoculated my 1st agar plates 3 days ago and did not know what I should be looking for. Your video made it clear that seeing little or nothing at this stage is to be expected. Have you ever considered doing daily timelapse of plate development with the cultures you sell?
Thank you
Nice one! your colorado oyster plate made us loads of big juicy mushrooms btw! xL
I only use H2O2 as a Sporicide. Example: If I have a wild mushroom I want to clone I’ll wash it with peroxide before tearing it in half. In a contaminated dish I can understand wetting the dish with peroxide to deactivate existing mold spores and moving the wanted mycelium to a fresh plate.
I doubt you’ll have luck killing mold and leaving the mycelium on the contaminated plate.
Thanks for sharing Your knowledge! Learning a lot with your videos! I have a question please: how do you sterelize your plastic petri dishes?
Gary you are our teacher, your way of teaching us mycology is excellent, it is a science that I like very much, I am looking forward to your videos, because I know that at the end of the video I see myself progressing in knowledge of cultivation techniques.
One question please, can we have one or more contaminants masked in a petri dish?
What you do is instead of train saving a infected plate take a pic of each colony that's actually mycelium that you want and put it on a new plate all it takes is a spec and then you get a very rapidly growing new plate so you take a plate like that one with the contamination intake 10 needle dips and stab them into new plates and you got ten rapidly growing plates as a result rather than messing around trying to kill off aspergillus or some other nasty fungus
What’s tween? I must have missed that part.
Could you use one of those uv bacteria lights to kill bacteria on Petri dishes?
Hi Gary, you mentioned in another comment that you'd provide a follow-up video on peroxide application. Has that been complete, and if so, can you provide a link to the video? Thanks for all you do!
yes it didn’t really do much - I believe there are some uses but it didn’t help with plates
Quite interesting, I took some tissue pieces from shiitake caps and my agar soon started to go brown from where the pieces where put , now the shiitake pieces are growing mycelium and is growing slowly over the dark brown shadow looking colour in the agar dishes , interesting to see what will happen , I think it might take over so far growing every day stronger with mycelium each day , wish I knew what the was going on , definitely learn something from this as it’s only shiitake that is doing this , very interesting.
shiitake mycelium can brown out when it’s stressed - it’s normal and also a critical stage when making fruiting blocks 👍 it creates a “bark” so the mycelium is protected when exposed to the air
Hey, Gary. Have you tried to squirt a little wood glue or gel to incapacitate a Trichoderma colony?
no I usually seal it and bring it out back to the compost pile
Great vide man! question, are you using a specific type of marker to write on your plates? cause i have to put tape over mine so they don't wash of when spaying with alcohol.
I use lab grade sharpies for plates, “wet wipe” sharpies for jars and lids and regular sharpies on bags ( I think I just grabbed whatever was closest for this video hah)
amzn.to/3pEsoek these work well too if your having trouble with the alcohol
@@FreshfromtheFarmFungi So lab grade sharpies don't wash off when sprayed with alcohol?
@@FreshfromtheFarmFungi Ok thanks!
China markers work well.
hi Gary, Are there any books that are about culture medium recipes that you can recommend? Thank you
there is a whole section in this book “Radical Mycology” - I use this as a reference regularly amzn.to/3EHDH9R
thanks Gary, by the way! happy New Year
One question 🤔 does real vanilla negatively affect the growth of mycelium in agar and or liquid culture?
I Love your vids!!!
not sure - worth testing! Thanks for following along 🍄❤️🙂
@@FreshfromtheFarmFungi
So far 12 days in liquid culture and nothing positive...I think I'll skip trying the agar lol.
Thanks for your response! And vids!
My pre-poured aliexpress plates don't germinate :( the samples get dark and bacteria starts spreading within days
maybe try different media like PDA or MEA
😉
When you’re diluting… is the bacteria ‘heavier’ / ‘lighter’ than the mycelium, thus assisting in separation?
Im not sure if weight matters - It’s more about separating everything more
so less bacteria is in the same volume. There may be other ways to filter/separate but this method just relies on reducing numbers overall
Can you please tell me how to isolate single spore culture on agar from a spore print.
Waiting for reply 🙂
yes watch this video - ua-cam.com/video/vsJpjQhsDIM/v-deo.html the whole series breeding mushrooms from spores is an in depth look at this
Thank you so much ,it's been a great help.i am in my masters and doing project on hybridization ,if i have doubts in future where can I contact you?☺️
I poured some agar two days ago and have left them to sit to be sure that they were not contaminated before I dropped some spores on them. How long should I wait to be certain that they are good?
most organisms show up within 72 hours some molds can take an extra few days so 5 days would be very conservative- 3 days is typically what I wait
@@FreshfromtheFarmFungi thank you sir
Can you try making a Golden Oyster/King Oyster hybrid ?
yes I will try
@@FreshfromtheFarmFungi Nice, feel free to make video of the result would love to see it. Btw have you done a video on the results on your other hybridisation experiment couldnt find anything
It's there a way to determine whether you have a haploid or diploid colony?
Do you use peptone in your agar?
only ways are genetic testing or growing it until fruiting 👍 just have to go off of speculation especially with such concentrated amounts on one plate
there is peptone in the MEA plates
@@FreshfromtheFarmFungi do you mean that diploid gives fruits but haploid not?
@@vitaly5209 Yes.
I want to work with you.
Why do you have to do so many plates?
it’s a numbers game - more plates means more crosses means more chances to get a good phenotype
@@FreshfromtheFarmFungi so after you put to grain & then substrate you grow it out? If the mushrooms look similar how do you know it worked?
Completely off subject, how do you keep condensation from forming on your plates?
temperature stability - it happens when the air inside the dish is warmer then the air around it
@@FreshfromtheFarmFungi Thanks Gary! I simply increased the temp with a small heater and problem solved. Was it this video that you mentioned where to go to buy your LC direct or is ETSY the only way?
Oh my God use parafilm tape don't ever open up your damn tray! I don't care if you're sitting in front of a filter blower or not. By the way I've gone probably three years without one single infection and I've never used a laminar flow hood or one of those filters in my life outside of a biological lab where I used to have to. I built a great big still there box out of polycarbonate then that's all you need people spend way too much money on this hobby I never get infections and I don't spend anything
Too much meaningless talk