Centrifugation
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- Опубліковано 21 сер 2024
- This lecture explains about centrifugation technique principle.
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Centrifugation is a process that involves the use of the centrifugal force for the sedimentation of mixtures with a centrifuge, used in industry and in laboratory settings.This process is used to separate two immiscible liquids. More-dense components of the mixture migrate away from the axis of the centrifuge, while less-dense components of the mixture migrate towards the axis. Chemists and biologists may increase the effective gravitational force on a test tube so as to more rapidly and completely cause the precipitate ("pellet") to gather on the bottom of the tube. The remaining solution is properly called the "supernate" or "supernatant liquid". The supernatant liquid is then either quickly decanted from the tube without disturbing the precipitate, or withdrawn with a Pasteur pipette.[citation needed]
The rate of centrifugation is specified by the angular velocity measured in revolutions per minute (RPM), or acceleration expressed as g. The conversion factor between RPM and g depends on the radius of the sample in the centrifuge rotor. The particles' settling velocity in centrifugation is a function of their size and shape, centrifugal acceleration, the volume fraction of solids present, the density difference between the particle and the liquid, and the viscosity.
In the chemical and food industries, special centrifuges can process a continuous stream of particle-laden liquid.
Centrifugation is the most common method used for uranium enrichment, relying on the slight mass difference between atoms of U238 and U235 in uranium hexafluoride gas.
Source of the article published in description is Wikipedia. I am sharing their material. © by original content developers of Wikipedia.
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Wait. 8 years ago. 😱. Shomu sir has really worked hard. More power to you sir. Thankyou.
Thank you
Who can dislike such an amazing video and explanation...really shocking ....this guy helped me a lot in understanding so many topics including Bioinformatics, analytical techniques etc....really awesome....
Thank you so much for appreciating my efforts
I think I have been graduating from you 😅🤗🤗(thanku Sir for explaining in such a way. )although I have got admission in s.t Xavier's college ranchi. But we have to study from own and then we turn out to UA-cam where i got teachers like you. Who make us informative. It is commendable 💯💯💯💯you don't know that you are securing a lot of student's and their carrier 🤗🤗🤗🤗
Thank you so much for appreciating my efforts. All the best for future career
Watch it in 2x
Sir can u pls explain centrifugation without ppt...
Pls explain in white board..
I was in 8th class when this video was uploaded.. Hatts off to u
You're welcome
The text to this video states: "Centrifugation is a process that involves the use of the centrifugal force for the sedimentation of mixtures with a centrifuge" this is not true for the simple reason a rotating mass "EXERTS" a centrifugal force, it is not subject to such a force.
Watched this on 2x speed. Much better
Auspicious👏👏👍👍.It's really a fabulous presentation.Gr8👍👌
Thank you. Glad you liked my lectures
Am the only one wondering who the "hilarious king rapjal" is ?
Yes you are, he is an Indian comedian😅
@@ja3een_joshi As the whole world doesn't resume to India i don't think i am the only one wondering xD
@@joaoborges8920 xD xD
u r explaination is good sir...made me easy 2 learn..thank u sir
Very nice found helpful in my analytical exam
so good exapalnation
Thanku you sir❤
You're welcome. Glad to hear that you're getting benefit from my lectures
very good video and explanations as usual. Thank you so much Guylaine (Canada)
I missed your old class..
Amazing Explanation Sir 😎😎😘😘
Thank you
Thanks for sharing.
1 svedberg is equal to 10 to the power minus 13 not 10 to 13..
We are constantly taught that only centripetal forces like Ff and Fb act UPON an orbiting body like your orbiting particle which you show but there are no centrifugal forces ON the orbiting body itself. But you say repeatedly that there is also a centrifugal force Fc acting ON the particle? If so what could be exerting this Fc on the orbiting particle or molecule in the centrifuge?
Is it not more correct to say that the centripetal forces acting ON the particle are generated or donated by the liquid beyond the particle and that due to momentum conservation law a reactive centrifugal force Fc acts ON the liquid which donated the centripetal force and that there is NO centrifugal Fc ON the orbiting particle itself as you keep saying. The reactive Fc is ONLY ON the liquid beyond the particle which donated the centripetal force to the particle and equals negative Ff. The particle itself ONLY experiences the 2 centripetal forces.The 2 centripetal forces acting ON the particle namely Fb + Ff = Mass particle x W^2.R or Mp x W^2.R
Again, there is NO THIRD or centrifugal force acting ON the orbiting particle only ON the liquid beyond the particle equaling the negative of the centripetal force Ff donated by liquid to the particle. This need not appear in the equation given above.
The reason why large dense molecules travel outwards is that in the earlier stages the centripetal forces are not large enough to keep denser particles in a CIRCULAR orbit and there is a Velocity called terminal but is a form of PARTIAL ESCAPE VELOCITY. Its NOT due to a centrifugal force Fc ON the particle as you say.
Love you sir and thank you
You're welcome. Glad to hear that you are getting benefit from the videos
very helpful! thank you
Aap hindi language me v video bnaiye aapki videos bhut achchi h agar hindi me ho to kuch logo ke liye kafi beneficial hoga
Stay tuned. New videos are being uploaded on these topics
thumbs up 👍👍👍👍👌💪
+elpwopoyoisroor KID thank you
I am having a doubt in this video- '' Why the process of centrifugation fails when we put tea leaves in the mixture we are about to centrifugate?
Very helpful
Thank you
Really explanatory vedio
Thank you. Glad you liked my lectures
Sir....What is/are the reasons of generation of heat during centrifugation..
Friction may be..!!
@@biswajitchakraborty909 thank you ji..I thought but not sure about that..
Just excellent
Thank you
valo explain korechen dada .. thanks .
Sir which u repeating again and again that is viscosity not velocity both r very much differ
SO mainly how many types of preparative centrifugation?i am confused
When you see the other tab.. 😂
lol
Sir plzz can u explain centrifugation on board
Will do that video soon.
sir plzzzz we need it on board...plsss sir can u do it as soon as possible
Is it right to say 60s means=60★10^-13second
Sir I'm reading pathfinder publication book and watching your videos for preparation of CSIR NET Exam and I want to clear this exam from self study but now I'm doing Bsc in last year student .sir want to confirm from you that these books is right for me ......????
Read pathfinder publication books also
Sir, please explain these on your board... it's a request
How does a centrifuge motor stop? what is the force that stops the motor spinning?
It’s a brake
Thank you !!
Is it viscosity and not velocity?
Thnk u so much
Sir can u send the link of this web page... Plzz if u don't mind 😥
Please upload it in urdu.... plzzzzzzzzxxx
Some time when a lecturer goes with flow they made mistakes cause they are also a human beings not God. Stop pointing a finger on him. Atleast appreciate his intentions.
the svedberg unit is not 10-13sec its 10 raised to minus 13 sec
Not satisfied with this vedio ....plz explain it on board ..😔😔
👌👌👌👌👌👌👌👌👌👍👍👍👍👍 good
Your explanation regarding rate zonal centrifugation is wrong..
Particles will not stop at any point they just separate according to their velocity.If u keep on centrifuging it,ultimately all particles settled at the bottom that's why we have to stop rate zonal centrifuge within short period of time
myself jyoti subhash patil ,student of Msc biotechnology JVWU,scholar id - 202294
Clarity ledu sir
😢
Bhai hindi language m video daalo
sir if you are having a problem with your English language it doesn't matter, we understand hindi as well, please sir it becomes really difficult to understand your English
Your videos are unnecessarily long and boring .
+Gargi Bhardwaj I want you to watch some more before judging. Thank you though
I've already watched some of your videos n have also downloaded a few of them sir . That's vy I am saying that you should keep your content precise.
ohh have u any better videos????
Gargi Bhardwaj, why don't you try teaching if you are so confident!
chutiya hai kya be tu?
Voice is too low to hear clearly..this is the only drawback of your videos
U don't have any idea what u r talking about..
Mixed rate zonal centrifugation with isopycnic centrifugation
Why don't you try teaching then!