Difference between sequencing Coverage and depth. Depth vs Coverage. Why they are important?

[@gittestokvadbrix8846]

Concise and understandable video. It's great!

[@abumohammed9659]

Hi
You are amazing !!! I really appreciate your video and it helps me a lot to understand and remember these two concepts
Thank you very much mate !!!!

[@XploreBio]

Thanks.

[@muhammadmohsinarshad9023]

Simple but effective 👍

[@XploreBio]

Thanks

[@anishamoonat6741]

Ur videos are really very good

[@BryanJL]

thanks!

[@narbahadurkatwal7154]

Hi.can we know the mutation frequency from the WES data? For eg. If some sample contains wild type as well as mutated cells and we performed the wes. Is there possibility to know the mutaion frequency or non mutaion frequency?

[@muhammadakmal1414]

I subsampled my reads to get 100x coverage. I wonder if after assembly the coverage would be same? how to check it sequencing depth?

[@nirmalpatel5249]

Hello
Thenk you so much for the crystal clear method demonstration.
I have question regarding this. We can crushing in nitrogen then how much time we store these material??? Like one month or 15 days?
Any idea about this.

[@XploreBio]

You can crush in liquid nitrogen and then store at -80 for a month or even more.

[@nirmalpatel5249]

@@XploreBio can i try - 10° to -20° c

[@XploreBio]

I have not tested it at these temperatures but I am sure this is not advisable as the nucleic acid may degrade quicker.