How to calculate protein concentration from SDS PAGE Gel using Image J free image analysis tool

[@helmytamrela5085]

Hi.. Thanks for the explaination. For the volume loaded, is it the sample only or it's included with the dye? thanks

[@job506]

Many thanks. However, the subsequent rectangles are returning back and stalking on the first rectangle. What can I do, please?

[@bikidas5029]

How did you copy the rectangles

[@fooballers7883]

Thanks... I am at present trying to work with Apo A / Apo B .. would you at anytime going to present a worked example on this topic ? Thanks again for this concise tutorial.

[@OlimHayot]

thank so much !