In some cases the spike sample is about 50 to 100 times the known concentration. I analyze Cocoa samples I was at first having a problem with spike but following the 50 to 100 times I got my results
Thank you so much for very helpful video! I have one question. If I got 90% recovery for my protocol. Then, I got 10 ug/kg for my sample. Do I have to calculate back to 100% because of my protocol can detect only 90%.
Matrix effect is the concept that analytes will respond differently in different systems. Spike recovery is the most common way to measure whether a matrix effect is present. If the spike recovery is close to 100%, then you can conclude that the analyte behaves the same in your sample (which is spiked) as it did in the set of simpler solutions used for calibration. I hope that helps.
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Your chemistry videos help so much. Thank you! You explain the concepts in a way that is so easy to understand. You clearly have an important skill.
Well explained. Thank you so much
Thank you very much. Now I can go ahead and do my test.
Thank you for this video! Really helped a lot!
In some cases the spike sample is about 50 to 100 times the known concentration. I analyze Cocoa samples I was at first having a problem with spike but following the 50 to 100 times I got my results
Very helpful video !
Thanks!
Thank you so muchhhhh. it s help me a lot
Please make more video for us
Thank you so much for very helpful video! I have one question. If I got 90% recovery for my protocol. Then, I got 10 ug/kg for my sample. Do I have to calculate back to 100% because of my protocol can detect only 90%.
I have not understood how to determine the concentration of the analyte in the unspiked sample??
You must plug the reading for the unspiked sample into the calibration curve to obtain its concentration for the method of spike recovery.
Is there a difference between matrix effect and recovery percent, or are they one in the same?
Matrix effect is the concept that analytes will respond differently in different systems. Spike recovery is the most common way to measure whether a matrix effect is present. If the spike recovery is close to 100%, then you can conclude that the analyte behaves the same in your sample (which is spiked) as it did in the set of simpler solutions used for calibration. I hope that helps.
I liked your video a lot but since you talk too fast it was difficult to understand you in the first hearing
i thought this was a smash bros tutorial 😐
You are so cute!!
Too fast
Hello. If you find a video on UA-cam to be too fast, you can click on the settings icon at the lower part of the video and select the playback speed, setting it to slower playback.