How to estimate the size of nanoparticles from UV-Vis absorbance in Origin
Вставка
- Опубліковано 22 лип 2024
- #nanoparticles #originpro #sayphysics
00:00 How to measure particle size using UV- Vis spectroscopy?
1:20 How do you determine the size of nanoparticles?
3:10 How can absorption spectroscopy be used to determine the size of nanoparticles?
4:05 Why UV visible spectroscopy is used for nanoparticles?
5:00 How do you calculate UV concentration from absorbance?
5:20 Size of nanoparticles calculations in Origin
How to estimate the size of nanoparticles from UV-Vis absorbance in Origin
Exercise File (Origin File):
drive.google.com/file/d/1Vm6d...
In this UA-cam video, viewers will learn how to estimate the size of nanoparticles using UV-Vis absorbance data in Origin software. The video will provide a step-by-step guide on how to prepare and analyze the data, starting with the creation of a calibration curve using known nanoparticle sizes. Once the calibration curve is generated, the absorbance data of the unknown nanoparticle sample can be compared to the curve to determine its size. The video will also cover the importance of proper sample preparation, selection of appropriate instrument settings, and potential sources of error in the analysis. By the end of the video, viewers will have gained a solid understanding of the principles and techniques involved in estimating nanoparticle size from UV-Vis absorbance data, and be equipped with the skills to perform this analysis in Origin.
"Nanoparticle size estimation" "UV-Vis absorbance analysis" "OriginLab data analysis" "Nanoparticle characterization" "Spectrophotometry techniques" "Particle size measurement" "Nanostructure analysis" "Spectral analysis methods" "Data interpretation in OriginLab" "Nanomaterial characterization" "Size determination from UV-Vis spectra" "Particle size distribution analysis" "Nanoparticle synthesis and characterization" "Nanoparticle optical properties" "Nanoparticle size determination techniques" "Spectroscopic data analysis" "UV-Vis spectroscopy for nanoparticle analysis" "Nanoparticle size measurement methods" "Nanoparticle absorbance spectra analysis" "Data plotting and analysis for nanoparticles in OriginLab"
All UV-Vis Analysis Video Tutorials:
1. How to calculate band gap energy from UV-Vis absorption
• How to calculate band ...
2. Calculate band gap energy from UV-Vis absorbance
• Calculate band gap ene...
3. How to calculate band gap energy from UV-Vis reflection (DRS) data
• How to calculate band ...
4. How to calculate band gap energy from photoluminescence (PL) data
• How to calculate band ...
5. Band gap energy with baseline correction using Tauc plot from UV-Vis absorbance data
• Band gap energy with b...
6. How to estimate the size of nanoparticles from UV-Vis absorbance data
• How to estimate the si...
7. Nanoparticle size, morphology, concentration and distribution from UV-Vis absorbance data
• Nanoparticle size, mor...
8. Calculate concentration from UV-Vis absorbance using Beer-Lambert's law
• Calculate concentratio...
9. How to calculate Urbach energy from UV-Vis absorbance data
• How to calculate Urbac...
10. How to plot and normalize FTIR and UV-VIS data
• How to plot and normal...
11. Absorption coefficient α calculation from UV-Vis absorbance data
• Absorption coefficient...
Reference article:
Determination of Size and Concentration of Gold Nanoparticles from UV−Vis Spectra
Wolfgang Haiss, Nguyen T. K. Thanh, Jenny Aveyard, and David G. Fernig
pubs.acs.org/doi/10.1021/ac07...
Supplementary information:
pubs.acs.org/doi/suppl/10.102...
Dear sir,
What are the values of L1 and L2 for silver nanoparticles?
As I have mentioned that the values of L1 and L2 are obtained from the fitting of UV-Vis and TEM data of the metal NPs. For other types of NPs, these values are better to be obtained from such fitting. Thanks
@@SAYPhysics Can i use these values for L1 and L2 for my own UV-vis measurements as long as they are also colloidal gold NP in range 25-100, or do i need to find my own L1 and L2?
You can use these values. Thanks
@@SAYPhysics we need to Tem data for calculation?
You can use the L1 and L2 values, if your NPs are of the same nature i.e metal or metal oxide NPs. Thanks
ډير مننه سرجي
Welcome dear
Really good information for us ..
Thanku sir
Thanks for the appreciation dear
I am searching this type videos finally I gotten.. just because I am working on nonparametric
Great. Thanks
Could you please give me the reference article where lambda note mentioned as minimum at the start of spr? In Haiss’s article it is mentioned as 512nm.
In the video description, you will find some references. Thanks
Dear sir, are there any values for L1 and L2 for silver nanoparticles? since these values were fitted using Gold NPs. if I can use the L1 and L2 values directly, how can I justify it, since the values were not deduced from silver?
You may use these L values which will result in an approximate solution as these values differ slightly for metal NPs. However, it's better to use the values of Ls specifically for Ag NPs, to be accurate. Thanks
Sir can you tell me how to calculate data fitting of TEM and I'm getting 0peak and spr peak are with difference of large distance...means many peaks are obtained from my sample...
It seems your data is having noise, as SPR is a single peak phenomenon in the case of NPs. Thanks
Respected Sir,
Thanks for such a helpful video with convenient explanation.
Kindly tell me can I use the same values of L1 and L2 for Magnetic gold nanoparticles with magnetic core and gold shell?
Thanks for the appreciation dear.
Yes, you may use for an approximated result. You may see the following video as well for more accurate calculations and comparison.
ua-cam.com/video/fQTU-5gtCSQ/v-deo.html
Dear Sir, How to calculate/find Wyckoff positions of a catalyst like Wyckoff positions of Cd and S in CdS photocatalyst? Thanks
I'm sorry, I don't know about it how to do such calculations. Thanks
@@SAYPhysics Sir, Wyckoff positions mean atomic coordinates (fractional/cartesian coordinates). If you understand about it, then kindly respond to my question. Thanks
Can we calculate the particle size for non-spherical particles (nanorods)?
We can measure upto spheroid NPs only. For NRs, different equations are to be incorporated and this method doesn't work for it. You may see the following video to get more insight. Thanks
ua-cam.com/video/fQTU-5gtCSQ/v-deo.html
I dont have TEM machine then what are the values of L1 and L2 for silver nanoparticles
You may utilize these values of L1 and L2 as Ag and Au are not that much different. In order to be accurate, you may follow my next video in this playlist, whose link is provided as follows. Thanks
ua-cam.com/video/fQTU-5gtCSQ/v-deo.html
Is it possible to use this equation to measure particle size for solid gold particles deposited on glass?
This equation is exactly true for the colloidal metal NPs, however, can be approximated for deposited or diffused metal NPs. Thanks
I have a question that L1 and L2 values will be same for all nanoparticles
Yes, all metal NPs as well as metal oxide NPs. Thanks
Sir can you tell me my sample contain zbo and Ag2O particle can i calculate with same TEM fitting values
Ag, Au and Zn have slight differences, but you can approximate. Thanks
Sir, if TEM data is available for any sample, what is the requirement of estimating the size with UV-Vis spectra, as TEM would reveal the size ?
The values of L1 an L2 are taken from a general data of TEM. In such cases, UV-Vis data can provide more information than TEM. TEM is then not required. Thanks
@@SAYPhysics Thank You Sir.
sir plz can you explain why the peaks 5, 10 and 20 nm are not in order as 20nm is highest one and than 5nm is on second instead of 10nm sir plz I have defence tommorow
Both intensity and wavelength changes with the size of NPs. With an increase in the size, the x-axis experiences a red-shift. While the intensity is NPs nature dependent. Wishing you good luck for your defense. Thanks
how to find the value of L1 and L2 for Zno nps text please
Some statistical data of TEM is to be fitted for ZnO to find the values of L1 and L2 or some literature may help. However, the same L1 and L2 values can be used for ZnO too as both are oxide NPs. Thanks
Sir kindly upload lectures of Wkb approximation
Sure, I'll after perturbation theory and variation principle. Thanks
sir what if i need L1 and L2 values of carbon nanoparticles
In such a situation you'll have to correlate these values with the TEM data of the same NPs. thanks
Thank you sir😊
Honestly I don't get the usefulness of this method since we always in need of the TEM to calculate the particle size. Since I can measure the particle size of 100 nm with SEM, what's the purpose of using the UV-vis !!
Basically when we are dealing with the size of nanoparticles and we are trying to reduce the size of nanoparticles, then it is very hard to do the TEM again and again and you know the TEM is not always available and is of course very costly. On the other hand, UV-Vis technique is a very simple technique and with the help of this we can estimate that whether a size reduction is occurring or not. Thanks
Sir, mera 10 absorbance mai 500 nm cuo nanoparticles aya hai its right or wrong
I'm not understanding your message. Thanks
Aoa. Sir how you. Sir I need Nanotechnology slides which you prepared for us...
I'm fine dear Which slides? Do you mean the old lecture slides? Thanks
Yes sir old lectures..... you delivered to us at Kust.
Ok. I'll check. Thanks
Can we calculate the size of nanorods using this equation?
This treatment is good for spherical nanoparticles. For other morphologies, this tutorial will be helpful. Thanks
ua-cam.com/video/fQTU-5gtCSQ/v-deo.html
@@SAYPhysics Thank you sir
You're welcome dear
10 absorbance : 345 nm cuo nanoparticles its right or wrong
Your message is not clear to me. Rewrite complete message so that I can respond properly. Thanks
Anyway we need TEM to know the values of L1 and Lo
No. For metal NPs, these the optimized values. For other NPs, one will have to fit their data to get these values. Thanks
@@SAYPhysics ok thanks. What about metal oxides and metal sulphide? And where we can get optimized values
We'll have to search some literature or do the fitting ourselves. Thanks
Dear Sir,
How to find the value of L1 and L2?
Can you please describe it?
As I have mentioned that the values of L1 and L2 are obtained from the fitting of UV-Vis and TEM data of the metal NPs. For other types of NPs, these values are better to be obtained from such fitting. Thanks
@@SAYPhysics Okay but how to fit the TEM data to get the values for the same?
We'll have to search some literature or do the fitting ourselves. Thanks
@@SAYPhysics fitting for what?
Particle size?
For the specific category of NPs, their TEM data to be plotted with UV-VIS to get values of L1 and L2 from fitting. Please see the reference I have mentioned in the video description. Thanks
can I calculate the size manually?
Yes, but that will be a very rough estimate though. Thanks
@@SAYPhysics Thanks but I need to know it for my assignment hahaha
Better you go with the two methods I explained in my video tutorials. Thanks
most of the responses you have been giving to the people are just plain wrong.
If you could tell me what's wrong with my response, I'll be grateful to rectify myself. Thanks in advance ☺️
Hello sir can u tell me how to calculate oscillator strength from uv visible plot??
It's a good suggestion to make a tutorial on it.
From Researchgate
The concept of oscillator strength comes from the classical viewpoint that when an electron is bound to a nuclear framework and possesses perfect oscillating properties that the excitation probability of the electron would have an oscillator strength of unity.
If we consider the molecule as a classical oscillating dipole, the oscillator strength (f) is defined by the integral over the absorption band
f = 4.3 x 10^-9 *integral (ε) dν
Here ε is the extinction coefficient and ν is in wave numbers.
The oscillator strength is also related to the quantum mechanical quantity referred to as the transition dipole moment (er).
f = (8πmeν / 3he^2) |er|^2
Thank you so much sir for your support actually I hv explored so much tutorials but nothing got related to this .it would be helpful if you make tutorial on oscillator strength calculation from absorption spectra ,if u need data I can share with you that part I couldn't get how to find band width and divided by 2 ..
Thanks for the appreciation dear. Yes, you share the data and details about plot on sayphysics@gmail.com. Thanks