Because of COVID my science course at college are unable to do certain experiments like PCR and gel electrophoresis, so this account is a life saver for my assignment on them. Thank you!!!
Electrophoresis is the separation of molecules with the use of an electrical field. Every substance, usually a nucleic acid or a protein, has a pI. That's the Isoelectric point and it's a feature that comes from the amount of positive and negative radicals it contains around it's perimeter (if it's a protein) or in it (if its a nucleic acid. Positive radicals move towards the negative pole of the field and negatives - to the postive. If you submerge a gel with the proper pH containing these molecules in a medium that allows an electrical current to pass through, the current will pull the molecules along with it. Heavier molecules stay behind, lighter move faster, thus, a mixture is seperated. Now, the molecular weight (how heavy they are) of a molecule is also its signature. You identify the molecules you need by their molecular weight.
Thank you for showing the entire procedure , now I understood electrophoresis properly after seeing all the steps... reading it from the book doesn't clear the entire concept of it.
Not sure is the comments are still answered, but... Why do we need a PLATINUM wire electrode for the electrophoresis? What exactly will happen if one replaces this to an ordinary Copper wire electrode?
this method i trust because you can watch it develop and prove it visually. i don't trust people saying you can hand carry a sequencer and plug it into a laptop. that doesn;t make any sense.
The rubber seals? Bro...they come with seals? We've always had to do the blue tape walls. (when you don't know if the tray actually came with one and they were lost or if you never had them to begin with.)
Very intersting and helpful video! Quick misiterpretation question : In 5:45 you say to add 10 micro-L of control sample (DNA ladder) and then 20 μL of each of the other DNA samples, am I correct?
Because of COVID my science course at college are unable to do certain experiments like PCR and gel electrophoresis, so this account is a life saver for my assignment on them. Thank you!!!
Are your labs being used?
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@@godislove8740 nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnñnnnnnnnnnnñ9n
absolute lifesavers, i had this experiment as my lab report and this video helped me like no other ❤
Never understood how electrophoresis worked by just being told about it, nice to see it for real. Thank you, very thorough video! :)
Do u see Newton before studying his law?🙄🤔😄
Electrophoresis is the separation of molecules with the use of an electrical field. Every substance, usually a nucleic acid or a protein, has a pI. That's the Isoelectric point and it's a feature that comes from the amount of positive and negative radicals it contains around it's perimeter (if it's a protein) or in it (if its a nucleic acid. Positive radicals move towards the negative pole of the field and negatives - to the postive. If you submerge a gel with the proper pH containing these molecules in a medium that allows an electrical current to pass through, the current will pull the molecules along with it. Heavier molecules stay behind, lighter move faster, thus, a mixture is seperated. Now, the molecular weight (how heavy they are) of a molecule is also its signature. You identify the molecules you need by their molecular weight.
Thank you so much, i did it with lab mates and then come here watching it, completely clear understand it, be blessed much!!
Are you in college or school
Now I understood better reading book is useless after watching this animation it's clear my all concept Thanks for making this Video
which books are you learning for bsc zoology 1st year
Thank you for showing the entire procedure , now I understood electrophoresis properly after seeing all the steps... reading it from the book doesn't clear the entire concept of it.
Great video.
It helped me to better understand the concept of Gel Electroforesis.
Thanks ❤️
GEL ELECTROPHORESIS is really easy to understand after seeing this video
Thanks
it is nice.I got enough understand about laboratory session as biotechnology student and thank you!
R u BSC student
you forget to add Ethidium Bromide to the conical flask after heating the agarose solution.
Protips. Use thin wells for sharp bands. Use black paper to ease loading. You need only 1 tip if you wash ur tip in the buffer
Great, I have used same method in my research , it's very sample and very beneficial video, Thanks ♥️
Not sure is the comments are still answered, but... Why do we need a PLATINUM wire electrode for the electrophoresis? What exactly will happen if one replaces this to an ordinary Copper wire electrode?
this method i trust because you can watch it develop and prove it visually. i don't trust people saying you can hand carry a sequencer and plug it into a laptop. that doesn;t make any sense.
The rubber seals?
Bro...they come with seals?
We've always had to do the blue tape walls.
(when you don't know if the tray actually came with one and they were lost or if you never had them to begin with.)
Very intersting and helpful video! Quick misiterpretation question : In 5:45 you say to add 10 micro-L of control sample (DNA ladder) and then 20 μL of each of the other DNA samples, am I correct?
The addition of Ethidium Bromide before the solidification of agarose gel was missing.
Great video I think I will pass my lab test
Amazing video
Thank you☺I hope that can also do the same in future days✌
So u was a neet aspirant
why do you need to separate dna by charge after pcr? my boss says pcr can tell us everything that is what they use in forensic files.
Thank you so much❤... Plz continue to do more videos like this
Thank you! it is a helpful video for my experiment.
A bundle of thanks
May I use QG buffer in agarose gel instead of TBE buffer?
Why don't you mix agarose with etbr
hello，I want to ask that have you miss the EB or Gel Red?
I also want to know is it ok to skip EB?
شكراً جزيلاً thank you very much
I`m.. biotechnology student bsc.. i do'nt know please tell any project topic..what kind of project i.. do?
How can we see the DNA without exposing it to UV radiation???
for that your going to need a microscope
U can't see DNA in visible light
Do they add amplified DNA sample from PCR or only DNA sample?
But longer molecules of DNA will have more -ve charge then why it is behind shorter ones
In DNA fingerprinting! DNA from gel is transferred to......... For hybridization?
why etbr or any other visualization dye is not added?
Sir upload also video on elisa pcr chromatography centrifugation and dna fingerprinting...
its gud video but you did n't tell about the loading dye and tracking dye.Try to give complete concept about experiment...
Can we use 1X TBE buffer in to the tank
You didnt show elution.
I did the same but after cutting gel to extract the DNA, the concentration was very low, dose anyone knows how to increase it?
Amazing. Thank you 🙏
Won't the DNA molecules leave the walls created in the gel to swim in the TAE buffer ?
No, because they are being pulled in one direction by the current.
How about addition of ethidium bromide???
Yeah, I think they forgot to mention about it in the video..
How can we see the DNA without the EtBr i.e intercalating agent which was not being added in the Agarose gel ?
Indeed . Thatswhat i was thinking too . We used Gelred instead of EtBr.
Nivedita Mitra he did say that the gel is ready to be stained. Though he did not showed the process.
Loading dye is also called ETBR
yess loading dye is also called Etbr
Loading dye ****?????
Best way❣️
Great video
Superb👍👍
if can disply the procedure by words while play the video good
Where is the gelred or gelgreen?
Muchas gracias i understand very good thank You for help me.
Can I ask how many liters the microwave is ?
Thanks a lot 😊
tips on not piercing the gel when inducing the dna?
there are master mix reagents where we dont need to add tracking dye , but making gel without etbr i hv nvr seen
This is helping in reading
Can we use 2 micro- L dna ladder with 5 Micro- L of pcr product/sample?
Which loading dye did you use?
Ethidium Bromide
@@preethathilakan5402 😲 it's too toxic... It's not suggested for use, better SYBR Green
Thank you so much..
Thank you
Hey all! If anyone's interested, check out our channel to see Electro-Sep - a product that can capture DNA bands in an agarose gel!
Can we use TBE instead of TAE?
Yup
You forget put ethidiom bromaid in liqued gel befor rins it
Best to gain better knowledge about........
Awesome...
😊😊😊😉
Thanks alot.nice explanation with video...
Everywhere I go I found loads of Indians in the comment section
That is according to ur location set in the yt settings
These basterds are almost Avery where
best vedeo sir
Thanks
thank you kevane laayak nathi tu
yes u miss etbr
Wow great
uh did not added etbr
I think she broke the walls in the gell
its very intresting
Thnks..
What happens when we eat a DNA
Good
Thanks.
Nice👍👍
7:23 can we see it just by naked eyes ?
No, u need UV light
@@arnavgandhi2942 is dna Fluorescence ?
Why do I always say Methyl A instead of Poly A tail? Sheesh
شكرا
كنت اظن نفسي الوحيد هنا الذي يتكلم اللغة العربية
@@الهاكرالجزائري-ح5ح نو لست لوحدك
Thanks a lot
you are so cute and pcr goes firmly
Thanks.. helpful
Etbr?😶
ua-cam.com/video/Fv-vayq5MCA/v-deo.html (Watch this video for precautions)
Any class 12 Bois
اريد باللغه العربيه نفس التجربه
Plz explain it in Urdu
Try muting the video ☕
Pls add Turkish language
I'm from mit
In silico: ua-cam.com/video/BkTRYMjyatA/v-deo.html
Barkai wayy
Rmddu
average
it is nice.I got enough understand about laboratory session as biotechnology student and thank you!
thank you
Thanks
THANK YOU
Thanks so much
Thanks