There is an update to this video here: ua-cam.com/video/B0DOYpfdsyg/v-deo.html To learn about Köhler illumination in detail see my first video: ua-cam.com/video/XEE-el7vC5k/v-deo.html and my video about conjugate planes: ua-cam.com/video/Ai86SMBJqr8/v-deo.html
Ive been an amateur microscopist for 40 years , the use of the microscope has always amazed me and its huge diversity optical concoctions. phase contrast, epi, UV. I recently used an optical microscope capable of x6500 I thought oil x100 was the limit. . I didnt try that setting but I wonder if it used reverse convolution to compute the surface. It was camera based. This 3D print looks amazing. You have clearly dedicated serious thought and have the results worthy. Regards.
Thanks. I intend to make some videos on deconvolution in microscopy at some point. However, even with deconvolution it is hard to push the resolution beyond what you can get with a high quality x100 oil objective (without using special fluorescence techniques). These super-high mag scopes just make small things easier to see without straining your eyes - which is good - but they don't really push the resolution 'limit'. You get essentially 'empty mag' as this video demonstrates: ua-cam.com/video/2sWF_NHPzn8/v-deo.html
I have been trying to assemble Koehler optics for microscopes that didn't have it originally. I was confused about the fourier plane in the illuminator. This plus your prior Koehler video made it clear. Thanks! I have not seen anyone else discussing Koehler as a 'special case' of modulation in this plane. I imagine a future in which every microscope has structured illumination, plus a camera and something like Pardus. And a few of us will say, "I remember the dark ages, before Fourierfeldblende."
Thank you - and thanks for your support! I hope to be doing quite a bit more on Fourier optics and Abbe diffraction theory in future videos so hopefully you will enjoy those also - but first I need to do a few computer programming videos so I can bring viewers along with me on the journey.
There is an update to this video here: ua-cam.com/video/B0DOYpfdsyg/v-deo.html
To learn about Köhler illumination in detail see my first video: ua-cam.com/video/XEE-el7vC5k/v-deo.html and my video about conjugate planes: ua-cam.com/video/Ai86SMBJqr8/v-deo.html
A very good video of Köhler lighting! Also, the opening sound has changed.👀
Thanks.
Ive been an amateur microscopist for 40 years , the use of the microscope has always amazed me and its huge diversity optical concoctions. phase contrast, epi, UV. I recently used an optical microscope capable of x6500 I thought oil x100 was the limit. . I didnt try that setting but I wonder if it used reverse convolution to compute the surface. It was camera based. This 3D print looks amazing. You have clearly dedicated serious thought and have the results worthy. Regards.
Thanks. I intend to make some videos on deconvolution in microscopy at some point. However, even with deconvolution it is hard to push the resolution beyond what you can get with a high quality x100 oil objective (without using special fluorescence techniques). These super-high mag scopes just make small things easier to see without straining your eyes - which is good - but they don't really push the resolution 'limit'. You get essentially 'empty mag' as this video demonstrates: ua-cam.com/video/2sWF_NHPzn8/v-deo.html
I have been trying to assemble Koehler optics for microscopes that didn't have it originally. I was confused about the fourier plane in the illuminator. This plus your prior Koehler video made it clear. Thanks! I have not seen anyone else discussing Koehler as a 'special case' of modulation in this plane. I imagine a future in which every microscope has structured illumination, plus a camera and something like Pardus. And a few of us will say, "I remember the dark ages, before Fourierfeldblende."
Thank you - and thanks for your support!
I hope to be doing quite a bit more on Fourier optics and Abbe diffraction theory in future videos so hopefully you will enjoy those also - but first I need to do a few computer programming videos so I can bring viewers along with me on the journey.