Microscope Slide Prep: Observing Spores and Finding Clamp Connections

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  • Опубліковано 20 гру 2024

КОМЕНТАРІ • 40

  • @mycomomjeans
    @mycomomjeans Рік тому +5

    Thank you so much for this series. I took microbiology 20yrs ago and your microscopy series has been so helpful in refreshing and expanding my knowledge! Your work and content is much appreciated ! 🍄❤️

  • @johnharrison6009
    @johnharrison6009 Рік тому +3

    Hey Gary, Thank you so much! Again, it is such a pleasure watching a real pro demonstrating their expertise and proper techniques. Time to dig out the old microscope! Thanks again!

  • @tonystewart7624
    @tonystewart7624 Рік тому +2

    Thank you for sharing this video. I got a microscope a few months ago and it's been sitting idle. Now I'll feel more confident using it.

  • @sporgymushrooms9656
    @sporgymushrooms9656 Рік тому +1

    Gary, you da man! Thanks for the clamp connect and slide prep demo. Looking forward to those new cultures I picked up on your Etsy shop!

  • @MissBlackMetal
    @MissBlackMetal 7 місяців тому

    15:00 and 17:00 examples of clamp connections (when 2 hyphae mate and exchange DNA)

  • @momsmushroomsjodyfoster5786

    Let me know if you should start offering classes. I'm especially interested in breeding cordyceps

  • @RustyColonsCPA
    @RustyColonsCPA Рік тому

    Great info as always! Mushlove!

  • @avk-pg5rb
    @avk-pg5rb Рік тому

    This was great! Thank you for this video and the many more you have uploaded regarding breeding, genetics, and the scientific explanations you upload. Love all the videos and THANK YOU!

  • @anniinthewoods8287
    @anniinthewoods8287 Місяць тому

    Exactly what I am looking for! Thanks

  • @JimmyRogers-tl1iw
    @JimmyRogers-tl1iw 3 місяці тому

    What are you running on your scope to see through computer??? I have the same scope, I also have the camera but idk how to use it with the 10x ocular

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  2 місяці тому

      there is software on the amscope website you need to connect with the USB camera and “swap” one of the oculars with the lens of the camera

  • @momsmushroomsjodyfoster5786

    You've got an awesome variety of stuff on Etsy. I got the camo hat for my husband for Christmas. I had a random question. If I want mushrooms ready by the 1st of May when should I start it. I grow from spore or L.C. not kits..also can you suggest what ones do well in the tent together. What name some that grow under the same conditions plz. Thx Gary

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  Рік тому

      it usually takes about 2 weeks at each phase (2 weeks on agar, 2 weeks on grain, 2 weeks on bulk and 2 weeks to fruit) That being said, there can be setbacks with contamination or some will take much longer (shiitake for instance). Most mushrooms will grow well in the same tent around 65F

  • @stacibryant3353
    @stacibryant3353 Рік тому

    Much needed information. Thanks for sharing!!

  • @marvin216
    @marvin216 Рік тому

    Where do I find the video on how to isolate a single spore using a Pipette tip?

  • @م.مهاابراهيم
    @م.مهاابراهيم Рік тому

    Haw can I prepare bromothymol blue please? I have bromothymol blue powder

  • @themyceliumnetwork
    @themyceliumnetwork Рік тому

    can I borrow your compound microscope ?
    I'll bring it right back when I'm done.......

  • @mycologylids
    @mycologylids Рік тому

    Awesome content as always.

  • @saabitrishrestha2811
    @saabitrishrestha2811 6 місяців тому

    Thank you for this video. Could you also please make a video regarding the Basidiospore
    size, expressed as (ℓext.min)ℓmin-ℓmax(ℓext.max) × (wext.min)wmin-wmax(wext.max), and the
    average size as ℓM × wM, where “ext.min” .

  • @phillymontana
    @phillymontana Рік тому

    Thanks for the video Funguy. Quick question - what would be the benefit of looking at spores or mycelium through the microscope? Is there any information I could gather from that process? I've got a microscope and was wondering if I could improve my mycology by doing this exercise somehow.

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  Рік тому

      for breeding it helps find mated pairings by looking for clamp connections- it also helps to identify unknown mushrooms
      or unique strains because of spore characteristics

    • @PhilScottCanada
      @PhilScottCanada Рік тому +1

      @@FreshfromtheFarmFungi Please do a video on spore characteristics.I want to start using my microscope for this. Thx.

  • @davidcollett1847
    @davidcollett1847 Рік тому

    Thanks man just getting in to 🔬 just ordered one today

  • @tonystewart7624
    @tonystewart7624 Рік тому +2

    I don't know if you noticed but it looked like there was movement to the left of the last mycelium clamp connection displayed. Cool beans

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  Рік тому +1

      that looks incredible looking back on it thanks!

    • @tonystewart7624
      @tonystewart7624 Рік тому

      @@FreshfromtheFarmFungi I thought so too! You're most welcome. I hope you have fun exploring what it is.

  • @tims-pt1yy
    @tims-pt1yy Рік тому

    This topic screams for more 😁

  • @م.مهاابراهيم
    @م.مهاابراهيم Рік тому +1

    What is the Name of the stain??

  • @kidorhymes
    @kidorhymes Рік тому

    How about to get proper microscope more suitable for mycology ? :) Like old inverted Olympus CK with proper lenses, then u will be able to observe and work with everything on agar, event single spore isolation as u got plenty of space from above... Proper lenses can focus trough agar with no problem... we use them daily here and it's like light years away from classic ones

    • @FreshfromtheFarmFungi
      @FreshfromtheFarmFungi  Рік тому

      ah yes for the next series I can upgrade. These Amacopes are less than 200$ the Olympus start around 5k. I agree that they are better, but for an at-home mycologist that capital could go towards many other things while this scope works well enough (the “Honda civic” of microscopes as I have stated earlier) Olympia is like a Cadillac - thanks for the feedback and next time you bust that scope out hopefully you will thank the mushroom gods and have some gratitude 🙏🏻 MUSHLOVE 🍄❤️

    • @kidorhymes
      @kidorhymes Рік тому +1

      @@FreshfromtheFarmFungi Maybe some used one from ebay ? :) My was around 500 $ Basic series of new ones are around 1700. but yes for sure they are much more expensive, but also much more suitable for the job :) And if u will get one with micro manipulators u can forget on serial dilution as is really easy to pick spores or anything else :)

  • @davedivesdeeper
    @davedivesdeeper Рік тому

    Mush love!

  • @InconspicuousOwl
    @InconspicuousOwl Рік тому

    Nice.

  • @coloradodirtbike5930
    @coloradodirtbike5930 Рік тому

    Too cool 😎