Microscope Slide Prep: Observing Spores and Finding Clamp Connections
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- Опубліковано 28 вер 2024
- **Disclaimer** Because of the nature of microscopy and this video, any claims are pure speculation. Multiple slides were used and no confirmatory testing was performed. This highlights the procedures used.***
This video breaks down a few different procedures for preparing slides for microscopy in mycology. Three common methods: Wet Prep, Heat Fixing and Scotch Tape Prep are used to observe spores and mycelium under the microscope. Enjoy and MUSHLOVE!
Amscope link
AmScope - 40X-2500X LED Digital Binocular Compound Microscope
amzn.to/3j93sLw
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Thank you so much for this series. I took microbiology 20yrs ago and your microscopy series has been so helpful in refreshing and expanding my knowledge! Your work and content is much appreciated ! 🍄❤️
thanks for watching and following along!
Hey Gary, Thank you so much! Again, it is such a pleasure watching a real pro demonstrating their expertise and proper techniques. Time to dig out the old microscope! Thanks again!
Thank you for sharing this video. I got a microscope a few months ago and it's been sitting idle. Now I'll feel more confident using it.
This was great! Thank you for this video and the many more you have uploaded regarding breeding, genetics, and the scientific explanations you upload. Love all the videos and THANK YOU!
Thanks man just getting in to 🔬 just ordered one today
What are you running on your scope to see through computer??? I have the same scope, I also have the camera but idk how to use it with the 10x ocular
Awesome content as always.
This topic screams for more 😁
Haw can I prepare bromothymol blue please? I have bromothymol blue powder
Mush love!
Where do I find the video on how to isolate a single spore using a Pipette tip?
It’s going to drop this weekend 🙏🏻
You've got an awesome variety of stuff on Etsy. I got the camo hat for my husband for Christmas. I had a random question. If I want mushrooms ready by the 1st of May when should I start it. I grow from spore or L.C. not kits..also can you suggest what ones do well in the tent together. What name some that grow under the same conditions plz. Thx Gary
it usually takes about 2 weeks at each phase (2 weeks on agar, 2 weeks on grain, 2 weeks on bulk and 2 weeks to fruit) That being said, there can be setbacks with contamination or some will take much longer (shiitake for instance). Most mushrooms will grow well in the same tent around 65F
can I borrow your compound microscope ?
I'll bring it right back when I'm done.......
Thank you for this video. Could you also please make a video regarding the Basidiospore
size, expressed as (ℓext.min)ℓmin-ℓmax(ℓext.max) × (wext.min)wmin-wmax(wext.max), and the
average size as ℓM × wM, where “ext.min” .
How about to get proper microscope more suitable for mycology ? :) Like old inverted Olympus CK with proper lenses, then u will be able to observe and work with everything on agar, event single spore isolation as u got plenty of space from above... Proper lenses can focus trough agar with no problem... we use them daily here and it's like light years away from classic ones
ah yes for the next series I can upgrade. These Amacopes are less than 200$ the Olympus start around 5k. I agree that they are better, but for an at-home mycologist that capital could go towards many other things while this scope works well enough (the “Honda civic” of microscopes as I have stated earlier) Olympia is like a Cadillac - thanks for the feedback and next time you bust that scope out hopefully you will thank the mushroom gods and have some gratitude 🙏🏻 MUSHLOVE 🍄❤️
@@FreshfromtheFarmFungi Maybe some used one from ebay ? :) My was around 500 $ Basic series of new ones are around 1700. but yes for sure they are much more expensive, but also much more suitable for the job :) And if u will get one with micro manipulators u can forget on serial dilution as is really easy to pick spores or anything else :)
15:00 and 17:00 examples of clamp connections (when 2 hyphae mate and exchange DNA)
What is the Name of the stain??
bromothymol blue you can also use lactophenol cotton blue
Haw can i prepare bromothymol blue please? I have the powder
Too cool 😎
Gary, you da man! Thanks for the clamp connect and slide prep demo. Looking forward to those new cultures I picked up on your Etsy shop!
thanks! yes hope they do well for you! HAPPY GROWING AND MUSHLOVE
Let me know if you should start offering classes. I'm especially interested in breeding cordyceps
Much needed information. Thanks for sharing!!
Great info as always! Mushlove!
Nice.
I don't know if you noticed but it looked like there was movement to the left of the last mycelium clamp connection displayed. Cool beans
that looks incredible looking back on it thanks!
@@FreshfromtheFarmFungi I thought so too! You're most welcome. I hope you have fun exploring what it is.
Thanks for the video Funguy. Quick question - what would be the benefit of looking at spores or mycelium through the microscope? Is there any information I could gather from that process? I've got a microscope and was wondering if I could improve my mycology by doing this exercise somehow.
for breeding it helps find mated pairings by looking for clamp connections- it also helps to identify unknown mushrooms
or unique strains because of spore characteristics
@@FreshfromtheFarmFungi Please do a video on spore characteristics.I want to start using my microscope for this. Thx.