Clean HPLC column by reverse flush method

In this video, I have demonstrated how to clean HPLC column. This is especially important to do if you add buffers or additives to your mobile phase. For instance most people use ammonium acetate, formic acid, etc. Over time, if the column isn’t flushed out regularly, precipitates can form and can plug your column up.
I am going to show you reverse flush method to extend your column life as much as possible.

[@venkataapparaokoyyana6691]

Nice Usha…..💐💐💐

[@syamalanakkella5831]

Good explanation...

[@ashrithavvs1227]

Very informative mam...thank you

[@tapani7590]

Nice presentation

[@prathushakorla7545]

Nice explanation mam

[@rohanplayes4689]

Good information 👍

[@33gireesha5]

Very clear experience.thank u mam

[@yashaswiniyadav6227]

Informative mam👍

[@sundeepsandy8640]

nice explantaion madam thank you very much and very useful for new one like me. i raise one doubt after complteation of this process column placed be same postion or reverse position while again use hplc .

[@jaithuammu3590]

Same position sir

[@vasanthreddy3720]

Very nice mam

[@akashkolhe1205]

Thanks mam 🙏

[@karthicks7495]

Thank you madam

[@44imranbasha67]

Nice 👏 mam

[@shahidabanu6222]

Can we use hot water for column.

[@user-zx6bx2nq3q]

Hello, I also degas the solvent in a water bath. At this time, I set the temperature to 25-28 degrees and then degas it. Does the temperature matter when degassing? thank you so much :)

[@madhupatnala]

Nice mam

[@preethisenapathi7284]

Very nice mam

[@sujitmore675]

Ppm solution prepration