+Neha Chandra i have done this experiment for 560nm OD & DNS is prepared by 1g of dinitrosalicyclic acid and 0.05g of sodium sulphite in 1% NaOH solution.... I guess
10gm of dns in200ml of 2N NaOH sol. Add this suspension carefully to 500ml of dist water containing 300g of sodium potassium tartarate ..make it 1000ml ..filter the sol. And store it in brown bottle
Explain estimation of glucose by Benedict method
how we can prepare the DNS reagent . please let me know the right protocol. and why we are taking od at 510 nm?
Neha Chandra because at 510 we can obtain more amount or correct amount of that how much carbohydrates is present in solution.
+Neha Chandra i have done this experiment for 560nm OD
& DNS is prepared by 1g of dinitrosalicyclic acid and 0.05g of sodium sulphite in 1% NaOH solution.... I guess
10gm of dns in200ml of 2N NaOH sol. Add this suspension carefully to 500ml of dist water containing 300g of sodium potassium tartarate ..make it 1000ml ..filter the sol. And store it in brown bottle
how can u make 300,400,500 microgram/ml solutions from 200 microgram/ml stock solution???
It's completely wrong!
And it is adviced to use micropipette...not this glass pipette...results are inaccurate with the use of this pipette
Pippette are more accurate than micropipette
It solves our purpose. Yes micro pipettes are alwas more accurate