How to process a z-stack image into a 2D image in ImageJ | How to process confocal microscope images

[@mahsamodareszadeh519]

Thank you for the video, would you please explain in comments how to make a 3d image (not max intensity) which we can turn like the window you showed in 1:43 (projection of organelles)?

[@AliceritaE]

I'll make a video to show you

[@johnalexis8819]

Madam, thank you a lot a lot for this video! It's so useful for my image selection and merging, thumbs up👍🏻

[@AliceritaE]

You're welcome 😊

[@joaopauloferreirarodrigues5576]

Fantastic!!!! Thank you Alicerita

[@AliceritaE]

You're welcome 😊

[@eduardoduarte1968]

Very nice! Thanks!

[@AliceritaE]

You're welcome!

[@917xyz]

very informative and useful. how to do the reverse, i.e. converting single 2D image into 3D?

[@AliceritaE]

I don't think a single 2D image can be project led into 3D. You will need more images at different depth to construct a 3D.

[@917xyz]

@@AliceritaE is it possible to assess depth on the basis of pixel contrast ?

[@AliceritaE]

Depth here is about capturing the image at different focus point. Just like different slices of a bread makes a loaf.